Field of the Invention
This invention relates to a Zebra fish egg collection device and in particular, this invention relates to a Zebra fish egg collection device wherein Controlled space is provided for mating of zebra fish using the simple glass device. Furthermore, this invention also relates to a fish egg collection device which has the beneficial effects of having simple structure, convenient to use, saving manpower cost, reducing labor intensity and having safety and reliability.
Background of the invention and the related Prior Art
Several fish species such as the medaka, fugu and zebrafish have become popular as models organisms for developmental studies. Some of the reasons for their popularity are, they are vertebrates (hence more significant to human research than invertebrate models such as fruit flies), and they have short developmental periods. And since these fishes lay eggs, they allow studies on development of the embryos (inside the eggs) outside of the mother's body. Of these models, the zebrafish is increasingly becoming the model of choice for many researchers. The egg laying in these fishes, can be manipulated using artificial light and periods as females lay their transparent eggs within about an hour after the light comes on (i.e., "dawn") after a dark cycle. An electronic

timer usually controls the light cycles in 14 h light and 10 h dark cycles in the environment in which the fish are housed. Noting the popularity of fish models, several manufacturers have developed aquatic housing where these models can be raised.
Gonsar N et al (2012) describes Zebrafish in our laboratory are usually bred by removing the fish from the recirculating aquatic system and placing them into 1-2L spawning tanks. These spawning tanks consist of a bottom reservoir, a lid, and an insert that fits in closely into the bottom reservoir. When the fish breed, the eggs fall through holes of the insert and into the reservoir, thus preventing them from being cannibalized. Because fish in these spawning tanks are not fed and do not get fresh water, they are bred only once a week. During a period where we had high demand for embryos, we instead tried breeding the fish for multiple consecutive days on the recirculating system. Fish were placed into the spawning insert as usual, but the insert was placed into the home tank instead of into the bottom reservoir. We found that there was no significant difference in the number of fertilized eggs produced between the spawning tank and home tank breeding methods. Further, the fish in the home tanks regularly produced fertile embryos over a 28-day time course, with the highest number of eggs per pair produced by the tank with only one pair of adult fish. This method is time-saving as fish bred in home tanks only require to be set up once. It is also an effective way to collect embryos over long periods from the same pair

or group of fish and to more easily obtain embryos from stocks with low spawning frequency.
U.S. Pat. No. 8327803 describes provide a method for processing a very large number of fish eggs for gene injection and so on, which employs a simple system with high performance and a reasonable construction expense. It is another object to provide the method having an water tank apparatus capable of producing a large number of fish eggs continuously. A large number of water tank groups having an independent drainage passage each is lighted up in turn with a predetermined interval. An egg-collecting case with a net bottom is set in the drainage passage connecting to the water tank group to which the lighting is started. The water tank group consists of a plurality of tank unit accommodating fishes each. Each book-shaped tank unit has a rectangular-shaped upper portion and a cone-shaped lower portion. Rotating water stream is formed in each tank unit.
US 20110174230 describes a fertilized eggs collection apparatus for injecting genes,., which is superior in safety and productivity. It is realized by simple structure. Fertilized eggs are collected from drainage with the fertilized egg in a water tank. The collection is synchronized for starting of illumination to the water tank. A probability that that feces included in the water is mixed in the collected fertilized eggs can hereby be reduced. Water including the fertilized

eggs is accumulated on a fertilized-egg-holding-plate. The fertilized-egg-holding-plate has each concave portion holding one fertilized egg each. Water in the concave portions is drained from a through-hole. The fertilized-egg-holding-plate is accommodated in a case having a bottom plate portion. The case keeps water for the fertilized eggs. A shutter plate capable of sliding is disposed on the fertilized-egg-holding-plate. The shutter plate prevents that the fertilized eggs jump out from the concave portions of the fertilized-egg-holding-plate.
|US7255064J refers to ]the collection of aquatic animal eggs during hatchery production is a critical step in the successful rearing of these animals. Currently, the equipment and culture methods do not allow large numbers of animals to be spawned at the same time without several people handling the process. The disclosed invention solves the problem of collecting large quantities of released aquatic animal eggs after spawning many adults at the same time. The apparatus is simple to build, maintain, and repair, and the method of its use requires only one person to handle the spawning of many adults.

CN202211078 discloses the automatic collection device for semi-buoyant fish eggs, which comprises a right-angle elbow pipe, a suspension rope, a mesh bag and an air inflation pipe. The right-angle elbow pipe is hung in a spawning pond through the suspension rope. The suspension rope is tied to the bent part of the right-angle elbow pipe. The right-angle elbow pipe comprises a vertical pipe segment and a transverse pipe segment. The lower end of the vertical pipe segment is provided with a heavy object, so that the vertical pipe segment is ensured to be vertical during the suspension process. The port of the mesh bag is sleeved onto the rear end of the transverse pipe segment and is tied up through a tying rope. The air inflation pipe is inserted into the vertical pipe segment at a certain length through the bent part of the right-angle elbow pipe. The automatic collection device is simple in operation, easy to use and low in production cost, which can reduce the labor intensity and improve the work efficiency. Due to the adoption of the automatic collection device, the damage to fish eggs is reduced and the collecting rate of fish eggs is high. Meanwhile, the hatching rate of fish eggs is also high. The automatic collection device is simple in structure and does not occupy the space of a spawning pond.
According to the document CN204217672 describes an adhesive fish egg collection device, and aims at providing an adhesive fish egg collection device, which has the advantages that the adhesive fish egg collection rate is high, the

structure is simple, and the convenience in use is realized. In the use process, the adhesive fish egg collection device is arranged in a cylindrical egg laying pool, and is used for collecting the adhesive fish eggs. The adhesive fish egg collection device comprises a cylindrical net cage, wherein the net cage is formed by detachably connecting a bottom surface and side walls, the upper end of the net cage is provided with an opening, the net tank is not higher than the water level of the egg laying pool, and the bottom surface and the side walls are respectively formed by detachably connecting a plurality of plastic net sheets. The adhesive fish egg collection device has the advantages that in the artificial propagation process of the adhesive fish eggs, the adhesive egg collection is convenient, the damage to the fish eggs is little, the hatching rate is high, the operation is simple and convenient, and the repeated use is realized. The patent document CN204616741 states that a fish cutlet box with a net pelagic egg collection device, including pocket, floater, plummet and handheld handle, pocket front end open -ended upper end is equipped with the floater, and the tip is equipped with the plummet under the pocket front end open -ended, and handheld handle lies in pocket front end opening both sides, the pocket is all open -ended shapes of front end and tail end, when collecting pelagic egg, the tail end for the opening rope tie up. This device adds the floater, and pocket upper portion will not sinks when collecting the roe, avoids the roe to run off, is fixed with the plummet simultaneously, lower tip will not

come -up, the maximum height of collecting the pocket be 50cm, and impurity such as parent population bottom the fish cutlet box with a net and excrement and urine are salvaged to the will not, can obtain high -quality roe, the collection finishes, unties pocket tail end opening rope, wash the roe in other containers can, with this device roe 98% or more in 2 -3 can collect the box with a net back and forth, its reasonable in design, easy operation, practicality are strong, buoyancy embryonated egg in here device mainly used collection fish cutlet box with a net.
Obviously, this problem is not new, and various attempts have been made to provide for collection of Zebra fish egg. None of these devices, however, have solved the problem of providing a portable and hygienic device for Zebra fish egg that is easy to carry and use and inexpensive to manufacture.
Summary of the invention
This invention relates to a Zebra fish egg collection device and in particular, this invention relates to a Zebra fish egg collection device wherein Controlled space is provided for mating of zebra fish using the simple glass device. Furthermore, this invention also relates to. a fish egg collection device which

has the beneficial effects of having simple structure, convenient to use, saving manpower cost, reducing labor intensity and having safety and reliability.
Detailed description of the invention with accompanying drawings
For the purpose of facilitating an understanding of the invention, there is illustrated in the accompanying drawings a preferred embodiment thereof, from an inspection of which, when considered in connection with the following description, the invention, its construction and operation, and many of its advantages should be readily understood and appreciated. The drawing is in one sheet.
Egg collection can be done on anesthetized or un-anesthetized animals depending on whether or not eggs are being collected for fertilization or attempting to relieve females with blocked ducts. Gloves may be worn but may also reduce sensitivity to safe handling including sensitivity to the degree of hand pressure on the animal. If gloves are not used, the user should thoroughly wash their hands in warm soapy water and thoroughly rinse prior to handling the fish.
The principal object of the invention is to provide a Zebra fish egg collection device.

The other embodiment of the invention is to provide a Zebra fish egg collection device wherein Controlled space is provided for mating of zebra fish using the simple, glass device.
The other object of the invention is to provide a Zebra fish egg collection
device in which adult zebra fish will be unable to enter into the bottom of the
tank as the space between two bended glass device are too small therefore the
device' prevent the fish to scavenge eggs.
The other object of the invention is to provide a Zebra fish egg collection
device which consists off; a) water tank with a top lead , b) supporting glass ; c)
aeration tube.
The other object of the invention is to provide Zebra fish egg collection device
which has the beneficial effects of having simple structure, convenient to use,
saving manpower cost, reducing labor intensity and having safety and
reliability.
The other object of the invention is to provide a Zebra fish egg collection
device wherein through the aeration tube sufficient amount of air/O2 may be
provided without disturbance of fish mating or disturbance of flow of fluid in
the tank.

The other object of the invention is to provide a Zebra fish egg collection device wherein eggs easily enter into the bottom of the tank and prevent the upward movement of eggs for disturbance of flow of fluid. The other object of the invention is to provide a Zebra fish egg collection device wherein the collection of fish eggs and the air inflation can be performed at the same time, while no extra investment is needed.
The other embodiment of the invention is to provide a Zebra fish egg collection device that has the advantages of simple structure and the like and time-saving.
The other embodiment of the invention is to provide the Zebra fish egg collection device in which the cost is low, the labor intensity is low. Zebra fish eggs are widely used now days for embryonic toxicity and antiangiogenics studies. Egg production depends on proper aeration for air/O2 supply without disturbance of flow of fluid, age of fish, size of fish, interval at which fish are used, light cycle, diet, and fish health status. Therefore it is importance to provide a suitable environment for mating as well as to create a suitable device for maximum collection of eggs and to prevent the adult fish from scavenging the eggs. In addition, none of the systems still available conveniently to provide suitable condition for fish housing with harvesting as the previous system either disturb the mating or unable to prevent scavenging

of eggs from male zebra fish and or the aeration system promote the eggs to be distributed all over the tank.
The device consists of a water tank with a top lead (1). The top lead connected with two bend supporting glass device plates (2). The supporting glass provides a suitable space for settle down the eggs into the bottom of the tank (3). In addition to this an aeration tube consisting of two parts one upper part is made up with solid open glass tube and the lower part with number of holes to arete air/O2 to the tank (4). On the top of the two supporting bend glass device two hole present to eject air (5). Free space is available for mating and egg production by the fish on the top of the bended glass device (6).
The novel features that are considered characteristic of the present invention are set forth with particularity in the appended claims. The invention itself, however, both as to its organization and its method of operation, together with additional objects and advantages thereof, will best be understood from the following description of certain specific embodiments, when read in connection with the accompanying drawings, in which:
FIG. 1 illustrates diagrams of the Zebra fish egg collection device of the present
invention;
Wherein the reference numerals of the fig 1 describes as;

1- Top lead device,
2- Supporting device;
3- Space for egg deposition;
4- Aeration tube with hole;
5- Hole to eject air;
6- Aeration tube solid.
Without further elaboration, the foregoing will so fully illustrate my invention, that others may, by applying current of future knowledge, readily adapt the same for use under various conditions of service. It should also be realized by those skilled in the art that such equivalent constructions do not depart from the spirit and scope of the invention.
Advantages over the prior art
The Zebra fish egg collection device proposed by the present invention has the following advantages over the prior art:
a) It is cost effective as compared to others device available in the market.
b) This device user friendly and easy to carry.
c) It has the characteristics of simple structure, convenient use, high efficiency.
d) It is economical in maintenances.

In the preceding specification, the invention has been described with reference to specific exemplary embodiments thereof. It will be evident that various modifications and changes may be made thereunto without departing from the broader spirit and scope of the invention as set forth in the claims that follow. The specification and drawings are accordingly to be regarded in an illustrative rather than restrictive sense. Therefore, the aim in the appended claims is to cover all such changes and modifications as fall within the true spirit and scope of the invention. The matter set forth in the foregoing description and accompanying drawings is offered by way of illustration only and not as a limitation. The actual scope of the invention is intended to be defined in the following claims when viewed in their proper perspective based on the prior art.

We claim:
1) A Zebra fish egg collection device which consists off:
a) water tank with a top lead , b) supporting glass ; c) aeration tube.
2) The Zebra fish egg collection device as claimed in claim 1 wherein Controlled space is provided for mating of zebra fish using this simple glass device.
3) The Zebra fish egg collection device as claimed in claim 1 in which adult zebra fish will be unable to enter into the bottom of the tank as the space between two bended glass device are too small to scavenge the eggs.
4) The Zebra fish egg collection device as claimed in claim 1 wherein through the aeration tube sufficient amount of air/O2 may be provided without disturbance offish mating or disturbance of flow of the fluid in the tank.
5) The Zebra fish egg collection device as claimed in claim 1 wherein eggs easily enter into the bottom of the tank and prevent the upward movement of eggs for disturbance of flow of the fluid.
6) The1 Zebra fish egg collection device as claimed in claim 1 wherein the top lead connected with two bend supporting glass device plates (2).
7) The Zebra fish egg collection device as claimed in claim 1 in which the aeration tube having two parts.

8) The Zebra fish egg collection device as claimed in claim 1 in which one upper part of aeration tube is made up with solid open glass tube and the lower part of aeration tube is with number of holes to arete air/O2 to the tank (4).
9) The Zebra fish egg collection device as claimed in claim 1 in which on the top of the two supporting bend glass device has two holes to eject air (5).
10) The Zebra fish egg collection device as claimed in claim 1 wherein free
space is available for mating and egg production by the fish on the top of the
bended glass device (6).