Description:FIELD OF THE INVENTION

The present invention is specifically applicable in the field of instrumentation for real-time and non-real time monitoring of target analyte concentration in samples at filed level. The invention focuses on a use and throw module developed for the colorimetric system for users to help in determining the concentration of target analyte.

5 The presented system is supported by seamless Internet of Things (IoT) technologies integrated with colorimetric unit to detect and transfer the data related to the concentration of analyte. The proposed system, specifically has the use and throw module which increases the accuracy and precision of estimating the concentration of analyte at field level rapidly with significantly reduced cost and time in simple user-friendly method.
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BACKGROUND DESCRIPTION AND PRIOR ART DISCUSSION

Colorimetry is one of the standard analytical technology used for qualitative and quantitative estimation of analytes in samples. Although there are miniature versions of the colorimetric devices in the market, still the user faces problems in determining the concentration of their target analyte. Some problems include non-applicability of the device at the field level, taking more time in analysis which in turn is contributed due to the laborious procedure and methodology resulting in low throughout and costly process.

25 Therefore it is important to have a rapid methodology which can be used at the field level at significantly reduced cost, methodology and time. In this context, many modern techniques are adopted few of them includes test-strips based products. Although test-strip based technology overcomes all the mentioned disadvantages, however, the range and precision are not as reliable as the one seen in the colorimetric based systems. There is no much research made in this direction to identify a technology that has the features of test-strip, specifically the use and throw feature, but it can be adapted in colorimetry so that the precision and accuracy part shall also be ensured.

OBJECTIVES OF THIS INVENTION
The objective of present invention is a developed user and throw module that simplifies the field based analytical test. Our innovation setup aims at a use and throw module with the colorimetric unit resulting in improved accuracy and precision in quantitative estimation of analyte. The innovation setup also includes an IoT module that is capable of transferring the data to the user in real time.

SUMMARY OF THE INVENTION
The present system contains use and throw module along with colorimetric unit for detection and quantification of analytes and transmission of same via IoT module to any centralized system. The use and throw module simplifies the entire colorimetric analysis down to few steps in low cost for estimation of any analyte at the field level.
Another important embodiment of our proposed system is a use and throw module with prefilled reagents with composition intended for quantification of analyte. These reagents, when reacts with analyte, develops a color whose intensity is proportional to concentration of analyte.
Another important embodiment of our proposed system is a use and throw module that has component(s) to facilitate injection of sample solution without any leakage during the analysis and injection process.
Another important embodiment of our proposed system is to transfer the data to user using IoT module or display the same in the device.
The last embodiment of the present invention is the methodology of operation:
- Wherein the sample is injected into the module
- The module is inserted into the colorimetric unit.
- The colorimetric unit then transfer the data to the user.
- Additionally, the calibration, if required shall be performed before inserting the module containing sample. The calibration process involves use of two additional modules one filled with blank solution and another with target analyte of known concentration.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to illustrate the proposed system, the attached diagrams clearly depict the realization of the methods and technologies incorporated in achieving the desired objectives in the present invention.

FIG. 1 set forth a block diagram of the device for quantitative estimation of analyte concentration.
FIG. 2 sets forth a diagram for use and throw module.
FIG. 3 sets forth a diagram for colorimetric unit.
DETAILED DESCRIPTION OF INVENTION

The present system has a use and throw module that has the opening or the facility wherein the samples are injected or flows in. The facility for opening is one way as shown in the figure 2 [046] which allows the only if suitably pieced through. Example 1, the opening shall contain inert material preferably vulcanized rubber or of similar material or composition that has similar physical features that shall be non-reactive with the chemicals present inside the module. This opening shall change its porous structure when it is subjected to sharp objects, preferably a syringe needle. As evident to the person skilled in the art, the nature and composition of opening shall be variable and the purpose of the example is only explanatory to convey the description of subcomponent. Example 2, the opening shall be suitable for micropipette to inject the samples directly into the module.

The present system has a use and throw module that is made of glass, preferably borosilicate glasses that has less effects on the absorbance and transmittance of light and related dissipation effects. The glass module as shown in figure 2 [873] is preferable to have the surfaces projected towards the light source and detector either plane or have a circular cross-section. For example, a cylindrical or cuboidal in shape.

The present system has a use and throw module size is variable but preferably comes with more than 0.5 cm in height or height more than sufficient enough for the light rays to pass through the module without significant deviation of light arising due to limitation in height size. For example, the glass module shown in figure 2 has the dimension of 2 cm in length and 0.5 cm in diameter with thickness of 0.5 mm.

The present system has a use and throw module with prefilled reagents which reacts with the target analyte and develops color proportional to its concentration. The glass module as shown in figure 2 [453] contains the reagents in dried form which reacts with the sample and develops color. These prefilled reagents will be in solid, liquid or in gaseous state.

The present system has a colorimetric unit used for sensing the concentration of analyte inside the use and throw module. The methodology used in determining concentration of sample in no way limits the scope of invention rather is described only for explanatory purpose. The calibration of system is as follows- the blank solution module is first filled without sample and the absorbance is taken whose value is recorded and fixed as the baseline reading. That is, the value of absorbance assigned to the variable X1 as 0 and the value of concentration assigned toY1 as 0. The next module containing known concentration of analyte is injected and its absorbance value is estimated and correlated to its percentage (For example, 15%). That is the value of absorbance X2 is say, 0.75 and Y2 =15%. The calibration process stops here and the analysis process begins with the third module. Then a third module is taken and injected with unknown sample is read in colorimetric device to retrieve its absorbance value X, whose Y value (% of analyte) is calculated by using the line equation
Y = mX,
Where m = (Y2 –Y1/ X2 –X1)
The calibration process is not required to be performed every time although recommended but not necessary except during the first time of operation after switching-on the module in a given round.

The present system has been explained with colorimetric sub-system as shown in the figure 3 [731] comprising of components as disclosed here. The opening [923] facilitates to house the module as described in the figure 2. The sub-system has at least one light source [798] and detector [381] preferably able to emit light of various wavelength or suitable systems are in place to selectively transmit particular wavelength. For example, one way to select the wave length is by using wavelength filter after light source. The light passes through the use and throw module and reaches detector which records the intensity. The IC ESP8266 transfers the data via WIFI to the user.

The present system contains chemicals inside the use and throw module which may contain chromogenic or color developing agent such as oxides and hydroxides of metal and its complexes, preferably potassium dichromate in acidified medium with catalyzers.

The present system has been explained with colorimetric system [731] as the unit for detection of concertation based on the absorbance and transmittance of use and throw module. As evident to the person skilled in the art, there are several other methods of detecting the absorbance with these use and throw modules disclose here. Some of those others includes RGB color code based smart-phone technology and direct USB transfer of the data to the smart-phone application. This in no way limits the applicability of module in quantitative estimation of analyte concentration to colorimetry as colorimetric method was used for explanatory purpose.

, Claims:We claim:

1. Disposable Module Based Ethanol Colorimetric Field Device for Gasoline-Blend consisting of disposable glass module (453) with enclosure (046) and a transparent glass tube (873) able to fit within the subsystem (731) containing the opening [923] in sub-system with at least one light source [798] and detector [381].
2. The device as claimed in claim 1, wherein said disposable glass module (453) contains pre-filled reagents capable of reacting with target analytes to generate a color indicative of analyte concentration.
3. The device as claimed in claim 1, wherein the opening (046) of said disposable glass module (453) comprises an inert material that changes its structure to allow injection of samples, facilitating a one-way entry for sample injection via a suitable instrument, such as a syringe needle or micropipette.
4. A disposable glass module (453) for colorimetric analysis, characterized by: a. Glass composition preferably of borosilicate material, b. A shape optimized for efficient transmission of light from at least one light source (798) to a detector (381), c. A dimension exceeding 0.5 cm in height to ensure unimpeded passage of light through the module.
5. The disposable glass module as claimed in claim 4, wherein said module contains prefilled reagents in dried or liquid form for reacting with target analytes, producing a color proportional to analyte concentration for estimation of ethanol in gasoline blends wherein the prefilled reagent may include chromogenic agent such as oxides and hydroxide of metal containing molecule.
6. A colorimetric unit (731) for detecting analyte concentrations within said disposable glass module (453), comprising:
a. At least one opening (923) to accommodate the disposable glass module,
b. At least one light source (798) and a detector (381) to measure the absorbance and transmittance of the sample within the module,
c. An IoT module for real-time data transmission to external systems or user devices.
7. The colorimetric unit as claimed in claim 6, wherein said unit includes a calibration process using blank solution and known analyte concentrations to establish a correlation between absorbance and analyte concentration for subsequent sample analysis.
8. A methodology for quantitative estimation of analyte concentration using the disposable module-based colorimetric field device, comprising steps:
a. Injection of a sample into the disposable glass module,
b. Insertion of the module into the colorimetric unit,
c. Measurement of absorbance and transmittance values, d. Data transfer to users via IoT or display on the device,
e. Optional calibration using blank and known analyte-containing modules.
9.A disposable module-based colorimetric field device, as claimed in any of the preceding claims, employed for the rapid and accurate estimation of analyte concentrations utilizes IC ESP8266 transfers the data via WIFI to the user