DESC:FIELD OF THE INVENTION
[001] The present invention relates to a composition to kill bacteria and viruses comprising of an antimicrobial bio-formulation. The present application further discloses a composition that is non-toxic, non-flammable, antimicrobial, sanitizing bio-formulation.

BACKGROUND OF THE INVENTION
[002] Antimicrobial components are used frequently to kill germs like bacteria thereby preventing various diseases. Alcohol-based sanitizers have various disadvantages such as being highly flammable, containing a large amount of ethyl or isopropyl alcohol thereby increasing the risk of alcohol toxicity, damaging the microbiome (healthy micro flora) of skin by killing off potentially beneficial bacteria and contributing to antibiotic resistance.
[003] WO02/102151 A1 discloses a sanitizer composition including (a) a sanitizing effective amount of an antimicrobial agent, (b) a water-soluble silicone, (c) a humectant selected from the group consisting of hydrogenated starch hydrolysates, glycerin, and mixtures thereof, and (d) water. This sanitizing composition is preferably substantially free of alcohol and more preferably contains less than about 0.5 or 1% by weight of alcohol, based upon 100% by weight of total composition.
[004] CN104287985A discloses a method for preparing a hand sanitizer. The method comprises the steps of sequentially dissolving a surfactant, propanediol phenyl ether, glycerin, benzyl alcohol, nipagin, cetanol and the like with deionized water at normal temperature, adding hydroxypropyl methyl cellulose after uniform dissolving, clarifying the solution, and adding methylchloroisothiazolinone, methylisothiazolinone and essence. The hand sanitizer is high in safety, low in foam and strong in washing capability, and particularly has double effects of washing and nursing hands with heavy oil stains.
[005] US20090082472A1 discloses a sanitizing composition or hand gel composition having a blend of SDA and Isopropyl Alcohol to kill germs and bacteria, a thickener, such as an acrylic polymer, a stabilizer or polymer acrylic acid neutralizer, such as polyoxyethylene coconut alkylamine, octyl isononanoate as an emollient, glycerin as an additional moisturizer, water, and optionally fragrance or other additives.
[006] WO2020070014A1 discloses a cleaning composition comprising at least one polypeptide having RNase activity, a method for cleaning an item and use of the cleaning composition. CN107197877B discloses a biological compound enzyme virus scavenger (disinfectant) with enhanced performance, which comprises proteinase K, trypsin and broad-spectrum nuclease, and the application is preferably textiles. US20190127665A1 discloses detergent compositions comprising polypeptide variants and methods of cleaning and/or treatment of surfaces using such compositions, and fabric treatment compositions comprising polypeptide variants. US20120219636A1 discloses a formulation and method for removing viruses from fresh produce, including at least one surfactant, and a solvent.
[007] Due to the various drawbacks in the prior arts as mentioned above, there is a need for a hand sanitizer which is non-flammable, non-toxic, healthy for skin, acts on various types of bacteria and virus, also keeps the healthy microflora on skin intact. The hand sanitizer disclosed by the present application is non-flammable, disinfects and/or sanitizes surfaces rapidly, and quickly dries after application.
[008] The primary object of the present disclosure is to provide a non-inflammable, antimicrobial sanitizing bio-formulation to kill bacteria and viruses. The said antimicrobial bio-formulation is alcohol free, non-toxic and quickly dries up upon application.
[009] Another object of the present disclosure is to provide a non-inflammable, antimicrobial sanitizing bio-formulation comprising of thermo stable protease, thermo stable lipase, micelle stabilizer, bio-surfactant, fatty acid, buffer components, RNase or DNase, stabilizer, moisturizer, iodophor and antimicrobial agent. The composition may optionally include thickening agent and skin healing agent.
SUMMARY OF THE INVENTION
[0010] In an aspect, the present disclosure relates to a non-inflammable, anti-microbial sanitizing composition comprising of thermo stable protease, thermo stable lipase, micelle enzyme stabilizer, bio-surfactant, fatty acid, buffer components, RNase or DNase based on virus type, stabilizer, moisturizer, iodophor and antimicrobial agent.
[0011] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing bioformulation, said bioformulation comprising of:
(i) protease 10-20 ppm;
(ii) lipase 10-20 ppm;
(iii) RNase or DNase 1-3 ppm;
(iv) iodophor 50-150 ppm;
(v) fatty acid 1-10 wt%;
(vi) antimicrobial agent 1-2 wt%, and
(vii) other additives and stabilizers.
[0012] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing bioformulation as described herein, wherein the other additives and stabilizers are selected from the group comprising of micelle stabilizer 20-30 ppm, bio-surfactant 500-700 ppm, stabilizer 2-4.3 ppm, buffer components 2-5 wt%, moisturizer 15-30 wt%, thickening agent 3-7 wt% and skin healing agent 1-10 wt%.
[0013] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing bioformulation as described herein, wherein the fatty acid is selected from the group consisting of glycerol fatty acid ester, or binary mixture of fatty acids including first fatty acid antimicrobial agent selected from C6 to C18 fatty acids; and wherein the anti-microbial agent is a quaternary ammonium antimicrobial agent and is selected from the group consisting of benzethonium chloride (BZT), benzalkonium chloride, methylbenzethonium chloride, benzoxonium chloride, PEG-12 dimethicon fluid, silicone polyether copolymer, dimethyl siloxanes and silicones and 3-Hydroxypropyl methyl.
[0014] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing bioformulation as described herein, wherein the micelle stabilizer is selected from the group consisting of Lauryldimethylamine-oxide, Cetyltrimethylammoniumbromide (CTAB), Sodium dodecylsulfate (SDS), Ammonium lauryl sulfate, or a mixture thereof; and wherein the bio-surfactant is selected from a group consisting of rhamnolipids, lecithin, sophorolipids, emulsion produced by Acinetobacter calcoaceticus or a combination thereof.
[0015] In an embodiment of the present disclosure, there is provided a non-inflammable anti-microbial sanitizing bioformulation as described herein, wherein the stabilizer is selected from the group consisting of D-glucuronic acid (6-carboxy-D-glucose), D-galacturonic acid (6-carboxy-D-galactose), N-acetyl-D-glucosamine (also N-acetylchitosamine), D-glucosamine (also chitosamine), N-acetyl-D-galactosamine (also N-acetylchondrosamine), and D- and L- fucose (6-deoxy-D- and L-galactose); water soluble polymer as stabilizer is selected from the group consisting of carbohydrate, polysaccharides, pullulan, chitosan, hyaluronic acid, chondroitin sulphate, dermatan sulphate, starch, dextran, carboxymethyl-dextran, polyalkylene oxide (PAO), polyalkylene glycol (PAG), polypropylene glycol (PPG), polyoxazoline, polyacryloylmorpholine, polyvinyl alcohol (PVA), polyethylene glycol (PEG), branched PEG, polysialic acid (PSA), starch, hydroxyalkyl starch (HAS) and hydroxylethyl starch (HES).
[0016] In an embodiment of the present disclosure, there is provided a non-inflammable anti-microbial sanitizing bioformulation as described herein, wherein the buffer components are selected from the group consisting of bicarbonate, buffer phosphate buffer, (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), N-(2-Acetamido)-aminoethanesulfonic acid acetate Salt of acetic acid, N-(2-Acetamido)-iminodiacetic acid, 2-aminoethanesulfonic acid, taurine ammonia, 3-(Cyclohexylamino)-propanesulfonic acid, 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid carbonate sodium carbonate, (Cyclohexylaminoethanesulfonic acid) Citrate Salt of citric acid, 3-[N- Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid Formate Salt of formic acid Glycine -Glycylglycine, N-(2-Hydroxyethyl)-piperazine-N’-3-propanesulfonic acid, 3-[N- Tris(hydroxymethyl)-methylamino]-2-hydroxypropanesulfonic acid Taurine 2-Aminoethanesulfonic acid, (Triethanolamine) and 2-[Tris(hydroxymethyl)- methylamino]-ethanesulfonic acid Tricine N-[Tris(hydroxymethyl)-methyl]-glycine Tris Tris(hydroxymethyl)-aminomethane.
[0017] In an embodiment of the present disclosure, there is provided a non-inflammable anti-microbial sanitizing bioformulation as described herein, wherein moisturizer is selected from the group consisting of mineral oil, propylene glycol, parabens, retinol, glycerol, squalane, hyaluronic acid, tea tree ethereal oil, deep-sea roe extracting solution, deep seawater, macadamia nut oil, spermaceti, and/or their hydrolyzed solutions and wherein the skin healing agents is selected from the group consisting of aloe vera, vitamin E, D-alpha tocopheryl acetate, DL-alpha tocopherol acetate, mixture of tocopheryls, alantoin, thiocitic acid, and apricot kernal.
[0018] In an embodiment of the present disclosure, there is provided a non-inflammable anti-microbial sanitizing bioformulation as described herein, wherein the thickening is selected from the group consisting of stearyl alcohol, cetyl alcohol, cetearyl alcohol, lauryl alcohol, biphenyl alcohol, oleyl alcohol, cetyl myristate, polyethylene glycol esters, polyethylene glycol mono stearate, polyethylene glycol distearate, carbomers and polyacrylic acid.
[0019] These and other features, aspects, and advantages of the present subject matter will be better understood with reference to the following description. This summary is provided to introduce a selection of concepts in a simplified form.
DETAILED DESCRIPTION OF THE INVENTION
[0020] Those skilled in the art will be aware that the present disclosure is subject to variations and modifications other than those specifically described. It is to be understood that the present disclosure includes all such variations and modifications. The disclosure also includes all such steps of the process, features of the product, referred to or indicated in this specification, individually or collectively, and any and all combinations of any or more of such steps or features.
Definitions
[0021] For convenience, before further description of the present disclosure, certain terms employed in the specification, and examples are collected here. These definitions should be read in the light of the remainder of the disclosure and understood as by a person of skill in the art. The terms used herein have the meanings recognized and known to those of skill in the art, however, for convenience and completeness, particular terms and their meanings are set forth below.
[0022] The articles “a”, “an” and “the” are used to refer to one or to more than one (i.e., to at least one) of the grammatical object of the article.
[0023] The terms “comprise” and “comprising” are used in the inclusive, open sense, meaning that additional elements may be included. It is not intended to be construed as “consists of only”.
[0024] Throughout this specification, unless the context requires otherwise the word “comprise”, and variations such as “comprises” and “comprising”, will be understood to imply the inclusion of a stated element or step or group of element or steps but not the exclusion of any other element or step or group of element or steps.
[0025] The term “including” is used to mean “including but not limited to”. “Including” and “including but not limited to” are used interchangeably.
[0026] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference.
[0027] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally equivalent products and methods are clearly within the scope of the disclosure, as described herein.
[0028] The present disclosure relates to a non-inflammable, anti-microbial sanitizing composition to kill germs like bacteria and viruses. The present invention further discloses a composition that is non-toxic, non-flammable, antimicrobial, sanitizing bio-formulation.
[0029] In an aspect, the present disclosure relates to a non-inflammable, anti-microbial sanitizing composition comprising of thermo stable protease, thermo stable lipase, micelle enzyme stabilizer, bio-surfactant, fatty acid, buffer components, RNase or DNase based on virus type, stabilizer, moisturizer, iodophor and antimicrobial agent.
[0030] By the term “Thermo stable” means retaining characteristic properties on being heated at 50 degree C.
[0031] RNase and DNAse are enzymes cutting RNA and DNA respectively.
[0032] Ribonuclease (RNase) is an enzyme that catalyzes the degradation of RNA into smaller components.
[0033] A deoxyribonuclease (DNase,) is an enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.
[0034] If intend is develop the formulation for RNA virus then RNase to be used.
[0035] If intend is develop the formulation for DNA virus then DNase to be used.
[0036] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein said anti-microbial sanitizing composition may optionally include thickening agent and skin healing agent.
[0037] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition comprising of:
(i) protease enzyme 10-20 ppm;
(ii) lipase enzymes 10-20 ppm;
(iii) RNase or DNase 1-3 ppm;
(iv) iodophor 50-150 ppm;
(v) fatty acid 1-10 wt%;
(vi) antimicrobial agent 1-2 wt%, and
(vii) other additives and stabilizers.
[0038] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition as shown in table 1 below, wherein the other additives and stabilizers are selected from the group comprising of micelle stabilizer 20-30 ppm, bio-surfactant 500-700 ppm, stabilizer 2-4.3 ppm, buffer components 2-5 wt%, moisturizer 15-30 wt%, thickening agent 3-7 wt% and skin healing agent 1-10 wt%.
[0039] Iodophor are any of a group of disinfectants containing iodine in combination with a surfactant. The inflammable, anti-microbial sanitizing composition comprises of at least one iodophor. Examples of iodophor includes but not limited to povidone, cadexomer iodine.
[0040] The fatty acids used in the present application have anti-microbial activity and are selected from glycerol fatty acid ester, or binary mixture of fatty acids including first fatty acid antimicrobial agent selected from C6 to C18 fatty acids.
[0041] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the fatty acid is selected from the group consisting of glycerol fatty acid ester, or binary mixture of fatty acids including first fatty acid antimicrobial agent selected from C6 to C18 fatty acids.
[0042] The antimicrobial agent is quaternary ammonium antimicrobial agent that provides activity against bacteria, germs and microorganisms. Quaternary ammonium antimicrobial agents are selected from benzethonium chloride (BZT), benzalkonium chloride, methylbenzethonium chloride, benzoxonium chloride, PEG-12 dimethicon fluid, Silicone Polyether Copolymer, Dimethyl Siloxanes and Silicones, 3-Hydroxypropyl Methyl.
[0043] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the the anti-microbial agent is a quaternary ammonium antimicrobial agent and is selected from the group consisting of benzethonium chloride (BZT), benzalkonium chloride, methylbenzethonium chloride, benzoxonium chloride, PEG-12 dimethicon fluid, silicone polyether copolymer, dimethyl siloxanes and silicones and 3-Hydroxypropyl methyl.
[0044] Micelle stabilizers in the composition keeps the protease and lipase enzymes in a neutral state until activation. The micelle stabilizers can be Lauryldimethylamine-oxide, or Cetyltrimethylammoniumbromide (CTAB), or Sodium dodecylsulfate (SDS), or Ammonium lauryl sulfate, or a mixture thereof. The bio-surfactants are selected from a group of rhamnolipids, Lecithin, Sophorolipids, Emulsion produced by Acinetobacter calcoaceticus or a combination thereof.
[0045] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the micelle stabilizer is selected from the group consisting of Lauryldimethylamine-oxide, Cetyltrimethylammoniumbromide (CTAB), Sodium dodecylsulfate (SDS), Ammonium lauryl sulfate, or a mixture thereof.
[0046] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the bio-surfactant is selected from a group consisting of rhamnolipids, lecithin, sophorolipids, emulsion produced by Acinetobacter calcoaceticus or a combination thereof.
[0047] The composition comprises of at least one stabilizer, selected from D-glucuronic acid (6-carboxy-D-glucose), D-galacturonic acid (6- carboxy-D-galactose), N-acetyl-D-glucosamine (also N-acetylchitosamine), D-glucosamine (also chitosamine), N-acetyl-D-galactosamine (also N-acetylchondrosamine), or D- and L- fucose (6-deoxy-D- and L-galactose) are monosaccharides. Water soluble polymer as stabilizer is selected from a carbohydrate, polysaccharides, pullulan, chitosan, hyaluronic acid, chondroitin sulfate, dermatan sulfate, starch, dextran, carboxymethyl-dextran, polyalkylene oxide (PAO), polyalkylene glycol (PAG), polypropylene glycol (PPG), polyoxazoline, polyacryloylmorpholine, polyvinyl alcohol (PVA), polyethylene glycol (PEG), branched PEG, polysialic acid (PSA), starch, hydroxyalkyl starch (HAS), hydroxylethyl starch (HES).
[0048] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the stabilizer is selected from the group consisting of D-glucuronic acid (6-carboxy-D-glucose), D-galacturonic acid (6-carboxy-D-galactose), N-acetyl-D-glucosamine (also N-acetylchitosamine), D-glucosamine (also chitosamine), N-acetyl-D-galactosamine (also N-acetylchondrosamine), and D- and L- fucose (6-deoxy-D- and L-galactose).
[0049] The buffer components are selected from Bicarbonate, Buffer Phosphate Buffer, HEPES, N-(2-Acetamido)-aminoethanesulfonic acid Acetate Salt of acetic acid, N-(2-Acetamido)-iminodiacetic acid, 2-Aminoethanesulfonic acid, Taurine Ammonia, 3-(Cyclohexylamino)-propanesulfonic acid, CAPSO 3- (Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid Carbonate Sodium carbonate, CHES Cyclohexylaminoethanesulfonic acid Citrate Salt of citric acid, DIPSO 3-[N- Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid Formate Salt of formic acid Glycine -Glycylglycine, HEPES N-(2-Hydroxyethyl)-piperazine-N’-ethanesulfonic acid HEPPS, EPPS N-(2-Hydroxyethyl)-piperazine-N’-3-propanesulfonic acid, TAPSO 3-[N-Tris(hydroxymethyl)-methylamino]-2-hydroxypropanesulfonic acid Taurine 2-Aminoethanesulfonic acid, AES TEA Triethanolamine, TES 2-[Tris(hydroxymethyl)- methylamino]-ethanesulfonic acid Tricine N-[Tris(hydroxymethyl)-methyl]-glycine Tris Tris(hydroxymethyl)-aminomethane.
[0050] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the the buffer component is selected from the group consisting of Bicarbonate, Buffer Phosphate Buffer, HEPES, N-(2-Acetamido)-aminoethanesulfonic acid Acetate Salt of acetic acid, N-(2-Acetamido)-iminodiacetic acid, 2-Aminoethanesulfonic acid, Taurine Ammonia, 3-(Cyclohexylamino)-propanesulfonic acid, CAPSO 3- (Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid Carbonate Sodium carbonate, CHES Cyclohexylaminoethanesulfonic acid Citrate Salt of citric acid, DIPSO 3-[N- Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid Formate Salt of formic acid Glycine -Glycylglycine, HEPES N-(2-Hydroxyethyl)-piperazine-N’-ethanesulfonic acid HEPPS, EPPS N-(2-Hydroxyethyl)-piperazine-N’-3-propanesulfonic acid, TAPSO 3-[N-Tris(hydroxymethyl)-methylamino]-2-hydroxypropanesulfonic acid Taurine 2-Aminoethanesulfonic acid, AES TEA Triethanolamine, TES 2-[Tris(hydroxymethyl)- methylamino]-ethanesulfonic acid Tricine N-[Tris(hydroxymethyl)-methyl]-glycine Tris Tris(hydroxymethyl)-aminomethane.
[0051] Optionally, the composition can include moisturizer selected from mineral oil, propylene glycol, parabens, retinol, glycerol, squalane, hyaluronic acid, tea tree ethereal oil, deep-sea roe extracting solution, deep seawater, macadamia nut oil, spermaceti, and/or their hydrolyzed solution.
[0052] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the moisturizer is selected from the group consisting of mineral oil, propylene glycol, parabens, retinol, glycerol, squalane, hyaluronic acid, tea tree ethereal oil, deep-sea roe extracting solution, deep seawater, macadamia nut oil, spermaceti, and/or their hydrolyzed solution.
[0053] Optionally, the composition can include thickening components comprising of higher fatty alcohols. Thickening components are selected from stearyl alcohol, cetyl alcohol, cetearyl alcohol, lauryl alcohol, biphenyl alcohol, oleyl alcohol, cetyl myristate; polyethylene glycol esters, such as polyethylene glycol mono stearate, and polyethylene glycol distearate. The thickening agents can optionally comprise of carbomers - the water immiscible components that can be used to form an emulsion. The carbomers can be polyacrylic acid.
[0054] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the thickening component is selected from the group consisting of stearyl alcohol, cetyl alcohol, cetearyl alcohol, lauryl alcohol, biphenyl alcohol, oleyl alcohol, cetyl myristate; polyethylene glycol esters, such as polyethylene glycol mono stearate, polyethylene glycol distearate and polyacrylic acid.
[0055] The skin healing agents can include aloe vera, Vitamin E, D-alpha tocopheryl acetate, DL-alpha tocopherol acetate, or mixture of tocopheryls, alantoin; thiocitic acid; or apricot kernal (Prunis Armeniaca).
[0056] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the skin healing agent is selected from the group consisting of aloe vera, Vitamin E, D-alpha tocopheryl acetate, DL-alpha tocopherol acetate, or mixture of tocopheryls, alantoin; thiocitic acid; or apricot kernal (Prunis Armeniaca).
[0057] Although the subject matter has been described in considerable detail with reference to certain preferred embodiments thereof, other embodiments are possible.
[0058] Having described the basic aspects of the present invention, the following non-limiting examples illustrate specific embodiment thereof.
[0059] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition as shown in table 1 below:
Components Range (Wt %)
Thermo stable protease 10-20 ppm
Thermo-stable lipase 10-20 ppm
Micelle stabilizers 20-30 ppm
Bio-surfactant 500-700 ppm
Fatty Acid 1-10 %
Buffer components 2-5 %
RNase or DNase 1-3 ppm
Stabilizer 2-4.3 ppm
Iodophor 50-150 ppm
PEG-12 dimethicone 1-2%
Optional components
Moisturizer 15-30 %
Thickening agent 3-7 %
Skin healing agent 10-50 ppm
Water To make 100%
Table.1: Composition of antimicrobial bio-formulation
[0060] In an embodiment of the present disclosure, there is provided a non-inflammable, anti-microbial sanitizing composition, wherein the role of components of the present composition are shown in table 2 below:
Components Role
Thermos table protease To hydrolyze peptide bonds of proteins
Thermo-stable lipase To hydrolyze triglycerides (fats) into their component fatty acid and glycerol
Micelle stabilizers Protects the protease and lipase form thermal degradation
Bio-surfactant Act as antibiofilm agents eradicating biofilms formed
Fatty Acid Highly effective antifungal reagents, ecofriendly
Buffer components To maintain pH of the formulation for better activity
RNase or DNase Digests single- and double-stranded DNA.
Hydrolyzes phosphodiester bonds of viruses in molecular level
Stabilizer Stabilizes the RNase or Dnase for effective function
Iodophor Act as antimicrobial agent and helps in easy penetration of RNase or Dnase to microbial body
PEG-12 dimethicone Helps for smooth feel, promote spreadability, and add lubrication
Optional components
Moisturizer Moisturizing, and lubricating the skin.
Thickening agent Thickens the formulation depending on use
Skin healing agent Vitamins for healthy skin
Table-2: Role of the components in the additive
EXAMPLES
[0061] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.

EXAMPLE-1
[0062] The antimicrobial bio-formulation was prepared as per the formulation described in Table 3 below.
Components Range
Thermo-stable bacteria protease 10 ppm
Thermo-stable bacterial lipase 10 ppm
CTAB 20 ppm
Rhamnolipids 500 ppm
Lauric Acid 2 %
Tris buffer 2 %
Commercial RNase 2 ppm
D-glucuronic acid 3 ppm
Povidone-Iodine 50 ppm
Benzalkonium chloride 2 wt%
Glycerol 15-30 %
PEG-12 dimethicone 1 %
Vitamin E 10 ppm
Water To make 100%
Table 3 Antimicrobial Formulation
[0063] The non-inflammable, anti-microbial sanitizing composition of the present disclosure, wherein the components of the present composition acts synergistically to give improved anti-microbial activity, as shown in table 4 below: The results of the below table shows that the present composition is effective in killing 99.9% of the microbes hence possess improved antimicrobial activity.
Code Components % Killing
A Thermos table bacterial protease 12
B Thermo-stable bacterial lipase 16
C CTAB 0
D Rhamnolipids 6
E Lauric Acid 7
F Tris buffer 0
G RNase 0
H D-Glucuronic acid 6
I Iodophor 11
J Benzalkonium chloride 16
K Glycerol 0
L PEG-12 dimethicone 3
A+B 17
A+D 8
A+E 18
A+H 15
A+I 12
A+J 21
A+L 8
B+D 21
B+I 12
D+J 28
E+I 15
H+J 26
H+L 8
A+B+C 28
A+B+I 13
A+B+J 23
B+C+I 15
B+C+J 22
A+B+C+D 26
A+B+H+I 18
D+H+I+J 41
A+B+D+H+I 43
H+I+J+K+L 41
A+B+D+H+I+J 52
A+B+E+D+H+I+J 52
A+B+D+E+H+I+J 59
D+E+D+H+I+J+K+L 44
A+B+C+D+E+F+H+I+J 68
A+B+C+D+E+F+G+H+I+J 74
A+B+C+D+E+F+G+H+I+J+K+L
(Optimized bio formulation) 99.9
Table.4: Synergistic effect of various components of bioformulation
[0064] The antibacterial activity of the present bio-formulation was calculated using control for 20 seconds expose time. The results are given in Table 5 below.
Microbe % Bacterial reduction control % bacterial reduction using antimicrobial bio-formulation as per table-2
E. coli 0 99.9
S. aureus 0 99.5
S. epidermidis 0 99.9
P. aeruginosa 0 99.9
S. typhi 0 99.9
C. tropicalis 0 99.9
Table 5. Anti-microbial activity of bio-formulation
[0065] The results of table 5 shows that the present bio-formulation is effective in killing microbes i.e., up to 99.9% bacterial reduction in a short exposure time of 20 seconds thus the present bio-formulation is having improved anti-microbial activity.
EXAMPLE-2: Antiviral activity of the bio-formulation
Plaque assay:
[0066] 150 µl of 1 X 106 pfu/ml SARS-CoV-2 was incubated with 150 µl of sanitizers for 10 sec at room temperature. As a control, 150 µl of culture medium (DMEM with 2% FBS) was incubated with sanitizers for 10 sec at room temperature. Mixtures were serially diluted to estimate SARS-CoV-2 titer by plaque assay on Vero E6 cell monolayer. Plaques were counted manually and reported.
Quality controls:
[0067] SARS-CoV-2 was incubated with culture medium (ratio1:1) was used as positive control. Uninfected cells were used as negative controls.
Assay validity criteria:
[0068] At least 10 plaques for counting in appropriate dilution in test samples and positive control. No plaques in negative control.
Sr. No Sample details Average titer (n=2) PFU/ml
1 Culture medium + SARS-CoV-2 (Control)
8.80E+05
2 9.60E+05
3 Bio-formulation + SARS-CoV-2
0.00E+05
4 0.00E+05
Table.6. Antiviral activity of bio-formulation

[0069] 10 second incubation of bio-formulation with SARS-CoV-2 completely inactivate virus.

EXAMPLE-3: Antibacterial effect of bioformulation on different surface
[0070] 2 x 2 cm size plastic, cloth, wood, glass, ceramic materials were washed with Millipore water and autoclaved at 121 degree C for 15 minutes. The anti-bacterial activity was calculated by standard method as described in ISO 22196:2011. The various bacterial cultured surfaces were sprayed with 0.1 ml of bioformulation (as given in Table-5, above) per 2 cm2. The controls were used to compare the % microbial reduction. The results obtained are shown in table 7 below.
Material % E. coli reduction % S. aureus reduction % S. epidermidis reduction % P. aeruginosa reduction % S. typhi reduction % C. tropicalis reduction
Control After spraying of present bioformulation (0.1 ml/2 cm2) Control After spraying of present bioformulation (0.1 ml/2 cm2) Control After spraying of present bioformulation (0.1 ml/2 cm2) Control After spraying of present bioformulation (0.1 ml/2 cm2) Control After spraying of present bioformulation (0.1 ml/2 cm2) Control After spraying of present bioformulation (0.1 ml/2 cm2)
Plastic 0 99.8 0 99.9 0 99.9 0 99.9 0 99.9 0 99.9
Ceramic 0 99.6 0 99.5 0 99.9 0 99.7 0 99.9 0 99.9
Wood 0 99.9 0 99.9 0 99.9 0 99.9 0 99.9 0 99.9
Cloth 0 99.9 0 99.9 0 99.6 0 99.9 0 99.9 0 99.9
Table. 7: Anti-microbial activity on various surfaces
[0071] The results of table 7 shows that the present bio-formulation is effective in killing microbes i.e., up to 99.9% thus the present bio-formulation is having improved anti-microbial activity.
,CLAIMS:WE CLAIM:
1. A non-inflammable anti-microbial sanitizing bioformulation comprising of:
(i) protease 10-20 ppm;
(ii) lipase 10-20 ppm;
(iii) RNase or DNase 1-3 ppm;
(iv) iodophor 50-150 ppm;
(v) fatty acid 1-10 wt%;
(vi) antimicrobial agent 1-2 wt%, and
(vii) other additives and stabilizers.

2. The anti-microbial bioformulation as claimed in claim 1, wherein the other additives and stabilizers are selected from the group comprising of micelle stabilizer 20-30 ppm, bio-surfactant 500-700 ppm, stabilizer 2-4.3 ppm, buffer components 2-5 wt%, moisturizer 15-30 wt%, thickening agent 3-7 wt% and skin healing agent 1-10 wt%.

3. The anti-microbial bioformulation as claimed in claim 1, wherein the fatty acid is selected from the group consisting of glycerol fatty acid ester, or binary mixture of fatty acids including first fatty acid antimicrobial agent selected from C6 to C18 fatty acids; and wherein the anti-microbial agent is a quaternary ammonium antimicrobial agent and is selected from the group consisting of benzethonium chloride (BZT), benzalkonium chloride, methylbenzethonium chloride, benzoxonium chloride, PEG-12 dimethicon fluid, silicone polyether copolymer, dimethyl siloxanes and silicones and 3-Hydroxypropyl methyl.

4. The anti-microbial bioformulation as claimed in claim 2, wherein the micelle stabilizer is selected from the group consisting of Lauryldimethylamine-oxide, Cetyltrimethylammoniumbromide (CTAB), Sodium dodecylsulfate (SDS), Ammonium lauryl sulfate, or a mixture thereof; and wherein the bio-surfactant is selected from a group consisting of rhamnolipids, lecithin, sophorolipids, emulsion produced by Acinetobacter calcoaceticus or a combination thereof.
5. The anti-microbial bioformulation as claimed in claim 2, wherein the stabilizer is selected from the group consisting of D-glucuronic acid (6-carboxy-D-glucose), D-galacturonic acid (6-carboxy-D-galactose), N-acetyl-D-glucosamine (also N-acetylchitosamine), D-glucosamine (also chitosamine), N-acetyl-D-galactosamine (also N-acetylchondrosamine), and D- and L-fucose (6-deoxy-D- and L-galactose); and wherein the water soluble polymer is selected from the group consisting of carbohydrate, polysaccharides, pullulan, chitosan, hyaluronic acid, chondroitin sulphate, dermatan sulphate, starch, dextran, carboxymethyl-dextran, polyalkylene oxide (PAO), polyalkylene glycol (PAG), polypropylene glycol (PPG), polyoxazoline, polyacryloylmorpholine, polyvinyl alcohol (PVA), polyethylene glycol (PEG), branched PEG, polysialic acid (PSA), starch, hydroxyalkyl starch (HAS) and hydroxylethyl starch (HES).

6. The anti-microbial bioformulation as claimed in claim 2, wherein the buffer components are selected from the group consisting of bicarbonate, buffer phosphate buffer, (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), N-(2-Acetamido)-aminoethanesulfonic acid acetate Salt of acetic acid, N-(2-Acetamido)-iminodiacetic acid, 2-aminoethanesulfonic acid, taurine ammonia, 3-(Cyclohexylamino)-propanesulfonic acid, 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid carbonate sodium carbonate, (Cyclohexylaminoethanesulfonic acid) Citrate Salt of citric acid, 3-[N- Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid Formate Salt of formic acid Glycine -Glycylglycine, , N-(2-Hydroxyethyl)-piperazine-N’-3-propanesulfonic acid, 3-[N- Tris(hydroxymethyl)-methylamino]-2-hydroxypropanesulfonic acid Taurine 2-Aminoethanesulfonic acid, (Triethanolamine) and 2-[Tris(hydroxymethyl)- methylamino]-ethanesulfonic acid Tricine N-[Tris(hydroxymethyl)-methyl]-glycine Tris Tris(hydroxymethyl)-aminomethane.

7. The anti-microbial bioformulation as claimed in claim 2, wherein moisturizer is selected from the group consisting of mineral oil, propylene glycol, parabens, retinol, glycerol, squalane, hyaluronic acid, tea tree ethereal oil, deep-sea roe extracting solution, deep seawater, macadamia nut oil, spermaceti, and/or their hydrolyzed solutions and wherein the skin healing agents is selected from the group consisting of aloe vera, vitamin E, D-alpha tocopheryl acetate, DL-alpha tocopherol acetate, mixture of tocopheryls, alantoin, thiocitic acid, and apricot kernal.

8. The anti-microbial bioformulation as claimed in claim 2, wherein the thickening is selected from the group consisting of stearyl alcohol, cetyl alcohol, cetearyl alcohol, lauryl alcohol, biphenyl alcohol, oleyl alcohol, cetyl myristate, polyethylene glycol esters, polyethylene glycol mono stearate, polyethylene glycol distearate, carbomers and polyacrylic acid.