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Fusion Protein
Abstract: The present invention provides a prospective means for use in a novel drug delivery system (DDS), an electronic device production, etc. More particularly, the present invention provides: a fusion protein containing (a) a ferritin monomer, and (b) a functional peptide inserted in a flexible linker region between a-helices of B- and C-regions in the ferritin monomer; and a polymer which has a lumen and is composed of a fusion protein containing (a) a ferritin monomer, and (b) a functional peptide inserted in a flexible linker region between a-helices of B- and C-regions in the ferritin monomer.
Notices, Deadlines & Correspondence
Patent Information
Applicants
AJINOMOTO CO., INC.
Inventors
1. INOUE, Ippei
Claims
1. A fusion protein comprising (a) a ferritin monomer, and (b) a functional peptide inserted at a flexible linker 5 region between α-helices in a B region and a C region of the ferritin monomer.
2. The fusion protein according to claim 1, wherein the ferritin monomer is a human ferritin monomer. 10
3. The fusion protein according to claim 1 or 2, wherein the human ferritin monomer is a human ferritin H chain.
4. The fusion protein according to claim 1 or 2, wherein 15 the human ferritin monomer is a human ferritin L chain.
5. The fusion protein according to claim 1, wherein the ferritin monomer is a Dps monomer. 20 6. The fusion protein according to any one of claims 1 to 5, wherein the functional peptide is a peptide capable of binding to a target material.
7. The fusion protein according to claim 6, wherein the 25 target material is an inorganic substance.
8. The fusion protein according to claim 7, wherein the inorganic substance is a metal material. 30 9. The fusion protein according to claim 6, wherein the target material is an organic substance.
10. The fusion protein according to claim 9, wherein the organic substance is a biological organic molecule.
11. The fusion protein according to claim 10, wherein the biological organic molecule is a protein. 5
12. The fusion protein according to any one of claims 1 to 11, wherein a cysteine residue or a peptide containing the cysteine residue is added to a C-terminus of the fusion protein. 10
13. A multimer comprising a fusion protein and has an internal cavity, the fusion protein comprising (a) a ferritin monomer, and (b) a functional peptide inserted at a flexible linker region between α-helices in a B region 15 and a C region of the ferritin monomer.
14. A complex comprising (1) the multimer according to claim 13, and (2) a target material, wherein the target material is bound to the functional 20 peptide in the fusion protein.
15. A polynucleotide encoding the fusion protein according to any one of claims 1 to 12. 25 16. An expression vector comprising the polynucleotide according to claim 15.
17. A host cell comprising the polynucleotide according to claim 15. 30
Specification
WE CLAIM:
1. A fusion protein comprising (a) a ferritin monomer,
and (b) a functional peptide inserted at a flexible linker
5 region between α-helices in a B region and a C region of the ferritin monomer.
2. The fusion protein according to claim 1, wherein the
ferritin monomer is a human ferritin monomer.
10
3. The fusion protein according to claim 1 or 2, wherein
the human ferritin monomer is a human ferritin H chain.
4. The fusion protein according to claim 1 or 2, wherein
15 the human ferritin monomer is a human ferritin L chain.
5. The fusion protein according to claim 1, wherein the
ferritin monomer is a Dps monomer.
20 6. The fusion protein according to any one of claims 1 to 5, wherein the functional peptide is a peptide capable of binding to a target material.
7. The fusion protein according to claim 6, wherein the
25 target material is an inorganic substance.
8. The fusion protein according to claim 7, wherein the
inorganic substance is a metal material.
30 9. The fusion protein according to claim 6, wherein the target material is an organic substance.
10. The fusion protein according to claim 9, wherein the
organic substance is a biological organic molecule.
11. The fusion protein according to claim 10, wherein the
biological organic molecule is a protein.
5
12. The fusion protein according to any one of claims 1 to
11, wherein a cysteine residue or a peptide containing the
cysteine residue is added to a C-terminus of the fusion
protein.
10
13. A multimer comprising a fusion protein and has an
internal cavity, the fusion protein comprising (a) a
ferritin monomer, and (b) a functional peptide inserted at
a flexible linker region between α-helices in a B region
15 and a C region of the ferritin monomer.
14. A complex comprising (1) the multimer according to
claim 13, and (2) a target material,
wherein the target material is bound to the functional 20 peptide in the fusion protein.
15. A polynucleotide encoding the fusion protein according
to any one of claims 1 to 12.
25 16. An expression vector comprising the polynucleotide according to claim 15.
17. A host cell comprising the polynucleotide according to claim 15. 30
Documents
Application Documents
| # | Name | Date |
|---|---|---|
| 1 | 202047035492-STATEMENT OF UNDERTAKING (FORM 3) [18-08-2020(online)].pdf | 2020-08-18 |
| 2 | 202047035492-PROOF OF RIGHT [18-08-2020(online)].pdf | 2020-08-18 |
| 3 | 202047035492-PRIORITY DOCUMENTS [18-08-2020(online)].pdf | 2020-08-18 |
| 4 | 202047035492-POWER OF AUTHORITY [18-08-2020(online)].pdf | 2020-08-18 |
| 5 | 202047035492-FORM 1 [18-08-2020(online)].pdf | 2020-08-18 |
| 6 | 202047035492-DRAWINGS [18-08-2020(online)].pdf | 2020-08-18 |
| 7 | 202047035492-DECLARATION OF INVENTORSHIP (FORM 5) [18-08-2020(online)].pdf | 2020-08-18 |
| 8 | 202047035492-COMPLETE SPECIFICATION [18-08-2020(online)].pdf | 2020-08-18 |
| 9 | 202047035492-FORM 3 [19-09-2020(online)].pdf | 2020-09-19 |
| 10 | 202047035492.pdf | 2021-10-18 |
| 11 | 202047035492-FORM 3 [27-11-2021(online)].pdf | 2021-11-27 |
| 12 | 202047035492-FORM 18 [21-01-2022(online)].pdf | 2022-01-21 |
| 13 | 202047035492-Correspondence_Form1_23-02-2022.pdf | 2022-02-23 |