ABSTRACT The present invention aims to provide a food containing, as a main ingredient, a foodstuff produced in developing 5  regions with many malnourished patients, specifically at least one kind of grain selected from the group consisting of soybean, maize and sorghum, which contains sufficient protein/amino acid n...

A method for preparing a polymeric protein which is composed of a monomeric protein produced by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure said method comprising: (A) a step of preparing the monomeric protein that has the form of granules insoluble in ...

There is provided a novel method for producing a dried instant soup, sauce or the like wherein at least one compound selected from the specified sulfur-containing compounds, which may be in the salt forms, such as glutathione and the like, is added to a feedstock composition in any stage during the production process ...

A bacterium belonging to the family Enterobacteriaceae, which has an ability to produce an amino acid such as L-cysteine, and has been modified to have a yeaS gene having a specific mutation is cultured in a medium, and the L-amino acid is collected from the medium to produce the L-amino acid.

ABSTRACT "Inosine derivatives and production methods therefor" The present invention relates to A method for producing an inosine derivative represented by general formula (1), wherein RI may be the same or different and are each benzyl group, benzhydryl group or trityl group, each of which may have a s...

In the process of producing a fish paste product, a difinite amount of a transglutaminase and ascorbic acid or a related compound thereof are added to material surimi. Thus, changes in the physical properties of kneaded fish paste with other ingridients nerimi can be suppressed during the process of producing a fish...

The present invention provides a novel sulfated polysaccharide having an anticoagulant activity. Specifically, the present invention provides the polysaccharide comprising a repetitive structure of a disaccharide unit composed of a hexuronic acid (HexA) residue and a a-D-glucosamine (GlcN) residue and having 13% or ...

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[Problem] The invention is to provide a practicable resin composition comprising constitutive elements of easily available materials and having well-balanced suitable curing capability and storage stability. [Means for Solution] The inventors have found that a compound having at least two epoxy and/or thiirane gr...

The present invention aims to provide an amino acid-containing granule preparation improved in the ease of taking medication than conventional products, which disintegrates rapidly. The amino acid-containing granule preparation of the present invention containing granules having a maximum particle size of substantiall...

The present invention provides an aromatic compound containing a sOecinc branch hi s aromatic compound containing a specific branch i s easily soluble in isopropyl acetate, which is excellent for separating liquids, and can be used for a method for producing peptides etc. that lead to the final product through extract...

This invention develops a novel technology that improves the secretion production of a heterologous protein using coryneform bacteria and provides a secretion production method for the heterologous protein. A coryneform bacteria is cultivated and a heterologous protein is secreted and produced. The coryneform bact...

Provided is a method for secreting and producing polymeric proteins which exploits novel techniques for improving the polymeric protein secretion and production capabilities of coryneform bacteria. Polymeric proteins are secreted and produced using as expression hosts coryneform bacteria which have polymeric protein...

The present invention provides a bacterium belonging to the family Enterobactriaceae, which is able to produce L-cysteine, and has been modified to decrease activity of the YdjN protein, or activities of the YdjN protein and the FliY protein.  This bacterium is cultured in a medium, and L-cysteine, L-cystine, a deri...

The present invention relates to a method for removing Fmoc groups the method comprising a step for mixing a compound represented by formula (I): HS L COOH (where L is an optionally substituted C alkylene group) a compound having amino groups protected by Fmoc groups and a base and obtaining a reaction mixture conta...

12121 221 221 221 221 22Provided is a highly safe cosmetic with an excellent whitening effect (which includes prevention and removal of spots). Further provided is a superior melanin production inhibitor. This cosmetic or melanin production inhibitor contains (A) a cysteine derivative represented by general formula ...

An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae. which has an L- amino acid-producing ability and inherently has an activity of a glucose dehydrogenase that uses pyrroloquinoline quinone as a coenzyme, but has been modified so that the activity of the glucose dehydroge...

Provided is a protected nucleotide for elongation that makes it possible to realize a method for producing oligonucleotides by the phosphoramidite process that allows purification to be conducted by liquid liquid extraction efficiently and at high yield. It was discovered that this can be achieved by using a specifi...

A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activgity such as (2S,3R,4S)- 4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate -dependent enzyme acti...

There are provided an L-cysteine-producing bacterium, and a method for producing L-cysteine etc. using the bacterium by developing a novel technique for improving L-cysteine-producing ability of a bacterium. By culturing a bacterium belonging to the family Enterobacteriaceae,  which has L-cysteine-producing ability an...

A method for manufacturing (2S,3R,4S)- 4- hydroxyl-L-isoleucine or a salt thereof using L-isoleucine producing bacterium transformed with a DNA fragment containing a gene conding for a protein having L-isoleucine dioxygenase activity and having a ability to produce (2S,3R,4S)-4-hydroxy-L-isoleucine. ...

Provided is a needle equipped connecting member (1), and a drug dissolution kit (35) using same comprising a member body (3) on which a hollow needle (2) is erected, and an elastic sheath (4) for covering the hollow needle (2), and having a drug solution container connection section (12) which is disposed on a side op...

The present invention provides for example: a chelated iron containing culture medium for neural stem cells and/or neural progenitor cells that promotes cell growth while maintaining the undifferentiation potency and multipotency of neural stem cells and/or neural progenitor cells; and a method for culturing neural st...

Disclosed is a method for imparting an aroma and/or a flavor to a food. Specifically disclosed is a composition for imparting an aroma and/or a flavor which contains one of (i) decanoic acid (ii) octanoic acid or (iii) one or more substances selected from among acetol furfural methyltetrahydrofuranone and 2 acetyl 5 m...

Disclosed is a flavor enriching agent comprising a substance discovered by searching for a number of variation compounds having a CaSR agonizing activity. Said substance exhibits an improved flavor enriching action particularly with respect to initial tastes exhibits an improved stability and can be produced by a simp...

A number of variation compounds having CaSR agonist activity were searched and a novel compound having superior action for imparting rich flavor was discovered. Provided are an agent for imparting rich flavor which comprises the abovementioned substance and a composite agent for imparting rich flavor which comprises t...

Provided is a protecting reagent which can undergo deprotection in high yield even under acidic conditions in organic syntheses e.g. peptide synthesis and which enables high purity products to be obtained. The protecting reagent is a benzyl compound represented by formula (I) which has only one hydroxy group substitut...

By developing a novel technique for improving the secretory production of a heterologous protein by a coryneform bacterium provided is a method for the secretory production of a heterologous protein. A heterologous protein is secreted and produced by culturing a coryneform bacterium which is capable of secreting and p...

The purpose of the present invention is to provide a medium for propagation of stem cells with good performance that reduces the use of albumin while maintaining medium performance stability. The present invention provides a medium for iPS cell propagation characterized by including a water soluble polymer such as pol...

The present invention aims to provide a compound superior in broad utility and stability, which is useful as a protecting reagent for amino acid and/or peptide in liquid phase synthesis and the like of peptide and an organic synthesis method using the compound, and provides a particular compound having a fluorene sk...

Provided is a method capable of manufacturing morpholino oligonucleotides efficiently and with high yields in a liquid phase synthesis method by using as starting material a morpholino nucleotide in which the 5 position hydroxyl group or a hydroxyl group present on a substituent of the position hydroxyl group has ...

The present invention relates to a dispersion-type feed additive composition for ruminants containing 40% by weight or more and less than 65% by weight of a basic amino acid which is a biologically active substance and a method of producing the same, and provides granules which have rumen bypass properties, can releas...

The present invention aims to provide a compound superior in broad utility and stability, which is useful as a protecting reagent (anchor) of amino acid and/or peptide in liquid phase synthesis and the like of a peptide having a C-terminal etc., which are of a carboxamide(-CONHR)-type, an organic synthesis reaction me...

The present invention provides a process for selectively-producing an enantiomer at position 4 of an optically active compound in the cross aldol reaction of pyruvic acid and indole-3-pyruvic acid. The process comprises the step of reacting pyruvic acid with indole-3-pyruvic acid in the coexistence of an optically ...

Disclosed are: a cysteine derivative which has a black melanin production inhibitory effect in addition to excellent stability and low odor; and a cosmetic preparation which contain the cysteine derivative. Specifically disclosed are: a cysteine derivative which is represented by general formula (I) or a salt thereof;...

According to the present invention, there are provided a pretreatment agent for a fish food which is able to inhibit lowering of the yield caused by heating and cooking the frozen food and which is able to keep a good palate feeling without affecting palate feeling and taste and accompanying unnatural feeling and tast...

The present invention provides crystals of phenylalanine derivatives of the formula (I), and particularly  y -type,  £ -type,  rj -type, and  9 -type crystals thereof.   These crystals are excellent in preservation stability or moisture resistance.  They can also be produced on the industrial scale. ...

ABSTRACT "An agent: for the prophylaxis or improvement of a functional gastrointestinal disorder" The present invention relates to an agent for the prophylaxis or improvement of a functional gastrointestinal disorder, which comprises, as an active ingredient, a compound selected from the group consisting of glutamic a...

A process for producing an inosine derivative represented by the following general formula (1), which comprises dithiocarbonylating an inosine derivative represented by the following general formula (3) and reducing the resultant compound with a radical. Thus, the target compound, which is useful as an antiviral agent...

The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an...

A comeyform bacterium which has an L-glutamic acid-producing ability and grows at least at the same growth rate as a non-mutated strain or a wild-type strain and has intracellular a-ketoglutarate dehydrogenase activity which is less than half that of the non-mutated or wild-type strain, and is obtained by introducin...

The present invention provides an agent and a food for the prophylaxis or improvement of a functional gastrointestinal disorder, which contains, as an active ingredient, at least one kind selected from glutamic acid, 5'-nucleotide and a salt thereof.

ABSTRACT "Inosine derivatives and production methods therefor" The present invention relates to a method for producing 2',3'-didehydro-2'.3'-dideoxyinosine represented by general formula (4), 0 (4) comprising the step of eliminating substituents RI, by using as an agent for eliminating the substituents selec...

The present invention provides a sweetener composition containing monatin or a salt thereof, which is undecomposable by-exposure to ultraviolet light or in an acidic condition, a preservation stabilizer of monatin or a salt thereof, and a method for preserving monatin or a salt thereof. According to the present inv...

ABSTRACT "GASTROINTESTINAL FUNCTION PROMOTER The present invention aims at providing a more effective gastrointestinal function promoter, specifically, an agent for the prophylaxis or improvement of functional gastrointestinal disorders, appetite regulator and the like and also a screening method for a substance...

A process for producing a phenylalanine derivative which has a quinazolinedione ring and is represented by the formula (5), which comprises reacting an acylphenylalanine derivative represented by the formula (1) with a reagent for carbonyl group introduction and an anthranilic acid derivative to form an asymmetric u...

Coryneform bacteria are described that have an ability to produce L-amino acids and are modified so that acetyl-CoA hydrolase activity is decreased. The bacteria are used to produce L-amino acids generated by biosynthetic pathway using pyruvic acid as an intermediate such as L-glutamic acid, L-arginine, L-glutamine,...

ABSTRACT A method for facilitating postoperative awakening of patients from anesthesia does not depend on the experience of anesthesiologists. Also provided are an anesthesia arousal composition for use in this method and a preparation containing a bicarbonate ion that facilitates awakening from anesthesia. Specifi...

A method for preparing a phenylalanine derivative having a quinazoline-dione ring represented by the following formula (l) or a pharmaceutically acceptable salt thereof, comprising the following steps (a), (b) and (c): with a carbonyl group-introducing reagent and a specific anthranilic acid derivative to thus form...

A pharmaceutical composition for preventing and/or treating diabetes, diabetic complication, hyperinsulinemia, disorders of glucose metabolism or obesity, comprising a combination of the following compound, analogues or pharmaceutically acceptable salts thereof with a hypoglycemic agent.

A solid dispersion or medicinal solid dispersion preparation which comprises a water-soluble polymer and either a phenylalanine compound represented by the formula (1) [wherein A represents the formula (2), etc.; B represents alkoxy, etc.; E represents hydrogen, etc.; D represents substituted phenyl, etc.; T, U, and...

ABSTRACT [Abstract] [Problem] To provide a gelling agent having a melting temperature of about 100°C, capable of solidifying a wide variety of oily base materials including silicone, and causing no "sweating" while retaining a practical level of gel strength. When applied to skin, it has good feeling, concretely th...

The present invention aims to provide a method of conveniently isolating and purifying as well as separating and removing (2S,3R,4S)-4-hydroxyisoleucine at a high purity and in a high yield. Specifically, the present invention discloses a purification method of (2S,3R,4S)-4-hydroxyisoleucine or a chemically acceptab...

The present invention relates to a method of producing L-glutamic acid comprising the steps of cultivating a coryneform L-glutamic acid-producing bacterium in a liquid medium, thereby producing and accumulating L-glutamic acid in a culture liquid, and collecting L-glutamic acid wherein said coryneform L-glutamic aci...

The body taste of a sweetener(forexample,aspartame,sucralose, asesulfame, etc.) is improved by blending a sweet substance with an amino acid or a peptide having an effect of activating a calcium receptor such as γ-Glu-X-Gly (wherein X represents an amino acid or an amino acid derivative), γ-Glu-Val-Y (wherein Y repr...

A low-fat food comprising an amino acid or a peptide having an effect of activating a calcium receptor such as γ-Glu-X-Gly (X represents an amino acid or an amino acid derivative), γ-Glu-Val-y (Y represents an amino acidor an amino acid derivative), γ-Glu-Ala, γ-Glu-Gly, γ-Glu-Cys, γ-Glu-Met, γ-Glu-Thr, γ-Glu-Val, y...

The present invention relates to a method for producing L-glutamic acid comprising culturing a coryneform bacterium having L-glutamic acid-producing ability in a medium so to cause accumulation of L-glutamic acid in the medium or the bacterium, and collecting L-glutamic acid from the medium or the bacterium, wherein...

A process is described for producing a catalyst for gas-phase oxidations, in which a suspension of TiO2 and V2O5 particles is applied to a fluidized inert support, wherein at least 90% by volume of the V2O5 particles have a diameter of 20µm or less and at least 95% by volume of the V2O5 particles have a diameter of ...

The present invention provides a method for producing L-arginine using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of one or several genes encoding an L-arginine transporter. ...

The present invention relates to a yeast extract is produced by using a strain of Saccharomyces cerevlslae, which can contain 1% by weight or more of y-Slutamylcysteine and contains 0.004 0.1% by weight of glutathione during its logarithmic growth phase, when the strain is cultured in a medium in which a glutathi...

A gel-state composition useful as, e.g., a composition for perfume cosmetics which is translucent to milk-white, is creamy to solid, has an excellent appearance, gives neither a tacky feeling nor a cooped-up feeling during use, and has excellent spreadability. The gel-state composition is characterized by containing...

By reacting inosinic add (IMP) with a bacterium which has been modified so that IMP dehydrogenase activity and 5-guanylic acid (GMP) synthetase activity are enhanced, GMP is produced.

Abstract The present invention discloses methods for producing a carboxylic acid chloride compound which comprises the step of reacting an alkyl-substituted cyclohexyl carboxylic acid with a chlorinating agent in the presence of a specific urea compound. According to this invention, it is possible to produce a carb...

The present invention discloses crystals of phenylalanine derivatives which have a specific structural formula, and particularly crystals which are easy to be used; crystals excellent in preservation stability, humidity resistance or solubility; and crystals which can be manufactured on the industrial scale. The pre...

The present invention provides a method for producing a protein which has restored a higher-order structure of a native state thereof: by bringing the protein which has lost a higher-order structure thereof into contact at pH 6.5 to 9.0 to a 1 to 3% aqueous solution of a specific surfactant, such as lauroylglutamic ...

The present invention relates to a cleansing composition, which comprises: (A) a sulfate anionic surfactant which is an alkyl sulfate selected from the group consisting of an ester salt of a straight chain saturated alcohol having 8 to 22 carbon atoms and sulfuric acid, an ester salt of a straight chain unsaturat...

The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modifi...

The inventors of this invention have conducted the search for a variety of compounds which may possess a desired CaSR agonist activity to thus find out a substance capable of imparting a kokumi, which shows a more excellent kokumi-imparting effect, in particular, a kokumi-imparting effect of the initial taste-impart...

Provided is a resin composition superior in the adhesiveness to a metal and having high organic solvent resistance, particularly, a resin composition preferable as a sealant for an organic electrolyte battery, which shows superior adhesiveness to a terminal or a collector made of a highly heat resistant metal such a...

The present invention relates to a multi-cell container having a partition wall capable of separately storing medicines, each of which is of powdered state and of mixing with each other under I simple and quick manner for providing a water solution. I [Means for Solving the Problem] I The multi-cell container i...

Solid compositions of sweetener with a high intense sweetness (including those in the form of solid food) having a sweetness quality with homogeneous dispersion in the whole, which comprise an aspartyl dipeptide ester derivatives such as N-[N-[3-(3-hydroxy-4-methoxyphenyl) propyl] -L-a-aspartyl]-L-phenylalanine 1-me...

A detergent composition which comprises (A) an N-(long-chain acyl) neutral amino acid or a salt thereof, (B) an N-(long-chain acyl) neutral amino acid dipeptide or a salt thereof, and (C) an N-(long-chain acyl) neutral amino acid tripeptide or a salt thereof at a specific weight ratio and which is excellent in the a...

A hydrolyzed protein which can be used as seasonings or the like is produced by heating and retaining an aqueous dispersion of a protein-containing starting material under an acidic condition with a plate-type heat exchanger using a liquid as a heat medium to make the aqueous dispersion substantially aseptic and the...

The present invention provides crystals of phenylalanine derivatives of the formula (I), and particularly alpha -type, gamma -type, epsilon -type, eta -type, and theta - type crystals thereof. These crystals are excellent in preservation stability or moisture resistance. They can also be produced on the industri...

The problem of the present invention is provision of a method of producing an n+p-mer oligonucleotide efficiently in 5 a high yield, which includes use of, as a starting material, an n-mer oligonucleotide wherein the 3"-terminal hydroxyl group is protected, and the 5"-terminal hydroxyl group is protected by a t...

The present invention provides a production method of a protected amino acid, protected peptide or peptide, including 5 precipitation and solid-liquid separation of C-protected amino acid or -C-protected peptide in a solvent containing watercontaining acetonitrile, after removing the N-terminal protecting group...

A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.

The present invention provides a production method of peptide, which includes the following step (1). 5 (1) removing N-terminal Fmoc group of N-Fmoc C-protected amino acid or N-Fmoc C-protected peptide wherein a C-terminal carboxy group is protected by an anchor group derived from an anchor soluble in halogenat...

A microorganism belonging to the family Enterobacteriaceae,  which has an L-amino acid-producing ability and has been modified so that the kdp system is enhanced, is cultured in a medium to produce and accumulate an L-amino acid in the medium or cells of the microorganism, and the L-amino acid is collected from the ...

Abstract A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY A method for producing L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, whe...

The present invention is characterized in that it provides: an additive for culturing stem cells, comprising a polysaccharide other than dextran sulfate having a molecular weight of 4,000 kDa-40,000 kDa; and a medium for culturing stem cells, comprising a polysaccharide other than dextran sulfate having a molecular ...

A method for enzymatic sulfurylation of a substrate is provided which includes the steps of reacting the substrate with 3"-phosphoadenosine-5"-phosphosulfate (PAPS) in a medium containing a bacterium belonging to the family Enterobacteriaceae to produce a sulfated derivative of the substrate, and collecting the sulf...

The present invention relates to: an additive and a medium which are for the suspension culturing of animal cells and contain a water-soluble polymer; and a method for the suspension culturing of animal cells, the method comprising suspension-culturing the animal cells in a medium that contains a water-soluble polym...

Provided is a protecting reagent which can undergo deprotection in high yield even under acidic conditions in organic syntheses, e.g., peptide synthesis, and which enables high-purity products to be obtained. The protecting reagent is a benzyl compound represented by formula (I), which has only one hydroxy group sub...

The purpose of the present invention is to provide a feed additive composition for ruminants the feed additive composition containing 30 wt% or more of a biologically active substance being a dispersed type which is highly protective in the lumens and exhibiting excellent dissolution in the gastrointestinal tract. T...

The present invention provides a method for purifying neural crest cells or corneal epithelial cells.

ABSTRACTIN/PCT/2 000/0045 4/CHE ANTITUMOR AGENTAn antitumor agent comprising a stilbene derivative and a platinum coordination compound as the active ingredients is provided. By employing these two types of active ingredients in combination, the antitumor activity is improved by a synergistic ef...

Specified phenylalanine derivatives and analogues thereof have an antagonistic activity to a 4 integrin. They are used as therapeutic 5 agents for various diseases concerning a 4 integrin.

The present invention relates to a morpholino oligonucleotide liquid phase synthesis method characterized in subjecting the reaction mixture of a condensation reaction to an extraction operation and isolating a product morpholino oligonucleotide in the organic layer. The present invention provides a method capable o...

The present invention addresses the problem of providing a means for achieving the previously difficult long term growth and cultivation of mesenchymal stem cells without using a special device equipment/materials etc. The abovementioned problem is solved by the provision of a culture medium for mesenchymal stem cel...

Provided are for example: a human serum albumin containing culture medium for the growth of neural stem cells and/or neural progenitor cells that promotes cell growth while maintaining the undifferentiation potency and multipotency of neural stem cells and/or neural progenitor cells; and a method for growing neural ...

The present invention provides an oligonucleotide production method that includes: (1) a step in which a reaction liquid comprising a phosphite triester body (c) is obtained by condensing, in a nonpolar solvent, a nucleoside, nucleotide, or oligonucleotide (a) in which the 5"-hydroxyl group is not protected and a nu...

A technique for efficiently culturing cells such as stem cells is provided.    Cells such as stem cells are cultured using a cell culture vessel coated with a fibroin-like protein inserted with a cell adhesion sequence containing RGD (Arg-Gly-Asp). [Figure 1]

The purpose of the present invention is to develop a new technique for improving secretory production of a heteroprotein by Corynebacterium and to provide a secretory production method for a heteroprotein. A Corynebacterium having the capacity to produce a heteroprotein by secretion and modified so as to reduce acti...

The present invention provides: a method for producing a heparosan compound having an isomerized hexulonic acid residue which has improved C5-epimerization efficiency; and a method for producing heparan sulfate. More specifically the present invention provides a method for producing a heparosan compound having an is...

The present invention provides a technique that enables modification of a soluble protein particularly regioselective modification of a soluble protein. More specifically the present invention provides a compound having a substance that has affinity for a soluble protein a cleavable moiety and a reactive group the c...

Provided is a medium for culturing pluripotent stem cells said medium containing L-tryptophan or an L-tryptophan derivative at a concentration of 176 µM or higher.

A method for producing RNA is provided. Objective RNA is produced by culturing a coryneform bacterium having an expression unit for the objective RNA which has been modified so that the activity of ribonuclease III is reduced in a medium to express the objective RNA and accumulate the objective RNA in cells of the b...

The present invention addresses the problem of providing a medium for growing and culturing pluripotent stem cells such as iPS cells and ES cells, in particular, a cell growth medium which is free from deterioration caused by the preservation of a complete medium, and a method for producing said medium. Provided are...

The present invention provides a prospective means for use in a novel drug delivery system (DDS), an electronic device production, etc. More particularly, the present invention provides: a fusion protein containing (a) a ferritin monomer, and (b) a functional peptide inserted in a flexible linker region between a-he...

The present invention provides a 2-OST mutant exhibiting a high activity. Specifically, the present invention provides a 2-O-sulfation enzyme mutant, having a substitution of a leucine residue at position 321 with a basic amino acid residue in any one amino acid sequence of: (a) the amino acid sequence of SEQ ID NO:...

Provided is a method for effectively inducing RNA silencing in an organism. RNA silencing is induced in the organism by treating microbial cells capable of producing an RNA for RNA silencing by using an organic solvent and then feeding the treated microbial cells to the organism.

The present invention provides an improver of the effect to increase the body weight of a farm animal or the efficiency of a feed, the improver being a coated-type improver which comprises a core that contains a polysaccharide having a property to coagulate a gram-negative bacterium and a coating agent for the core....

By developing a novel technique for enhancing the secretory production of a heterologous protein by a coryneform bacterium, provided is a method for the secretory production of a heterologous protein. A heterologous protein is secreted and produced by culturing a coryneform bacterium which is capable of secreting an...

The present invention provides a method for producing mesenchymal stem cells from a living body-derived cell sample containing mesenchymal stem cells, the method comprising: (1) a step for culturing a living body-derived cell sample containing mesenchymal stem cells in a serum-free medium in the presence of vitronec...

Provided is a method for producing a target protein using an animal cell as an expression host. A target protein is produced by culturing animal cells having the ability to produce the target protein under conditions of enhanced phosphoric acid concentration and/or potassium concentration in the medium. ...

Provided are: a method for producing a complicated peptide molecule, such as a dimeric peptide, in a simpler manner at lower cost compared with the conventional chemical synthesis methods; a dimeric peptide which comprises a specific short linker and has a satisfactory activity; and others.

The present invention provides a cell culture medium composition containing 150 ng/mL or more of basic fibroblast growth factor (bFGF).

The present invention provides a cell culture method that comprises: a first step for preparing a cell mass population having a long diameter of 400 µm or less; and a second step for suspension culturing the cell mass population obtained in the first step.

The present invention provides a medium having a reduced osmotic pressure, said medium comprising a basal medium and being characterized in that the osmotic pressure is adjusted to 150-250 mOsm/kg.

The present invention provides a cell culture method that comprises adding choline at a preset concentration to a medium.

The present invention provides a method for producing a target protein by using an animal cell as an expression host. According to the present invention, the target protein is produced by culturing animal cells having the ability to produce the target protein in the presence of a L-cysteine derivative such as (2RS,4...

The purpose of the present invention is to provide a medium for cell culture that permits cell culture without exhibiting toxicity when a high-concentration HEPES buffer is used, a method for producing said medium, and a cell culture method in which the medium is used. A medium for cell culture containing 4-(2-hydro...

Provided is a mutant transglutaminase (mutant TG) having high heat tolerance and/or high pH stability. This mutant TG has a mutation at an amino acid residue such as D1, Y24, R48, S101, G102, N139, D142, L147, K152, G157, R167, N176, K181, E182, H188, D189, R208, T245, S246, G250, G275, S284, H289, G301 or K327 and/...

The present invention provides a peptide delivery means capable of avoiding disruption of a cell membrane, immunogenicity, and toxicity. More specifically, the present invention provides the following (1) to (3). (1) A ferritin enclosing a peptide that comprises 3 to 19 amino acid residues and satisfies the followin...

ABSTRACTHerein is disclosed a process for producing a cysteine-rich food material, which comprises (ia) maintaining a food material containing y-glutamylcysteine at a ratio of at least 1 weight % to the solid content thereof (such as yeast cells or a yeast extract), at 50 to 120 °C and at pH 1 to 7 in ...

A medium for culturing stem cells containing albumin carrying a reduced amount of fatty acids due to fatty acid removal treatment or the like; a method for producing a medium for culturing stem cells characterized by preparing albumin carrying a reduced amount of fatty acids by subjecting albumin to fatty acid remov...

The invention provides a precipitation accelerator that is an organic compound having one or more straight chain aliphatic hydrocarbon groups having 10 or more carbon atoms the total number of carbon atoms in these aliphatic hydrocarbon groups being 20 or higher. This precipitation accelerator is used to cause organ...

A method for producing an alkylamine derivative represented by formula (I) and having a urea bond or a salt of the alkylamine derivative which comprises steps (a) and (b) described below. Step (a): (drawing) Step (b): a step for eliminating a protective group of the reaction product obtained in step (a) if necessary...

A feed for an aquatic organism and a method for culturing an aquatic organism are provided. An aquatic organism is cultured by feeding a feed for an aquatic organism to which an arginine derivative such as agmatine has been added to the aquatic organism.

The present invention provides a medium for neural stem cells and/or neural progenitor cells and substantially free of one or more amino acids selected from the group consisting of L-glutamine, L-asparagine and L-aspartic acid, and a method for culturing the neural stem cells and/or neural progenitor cells in the me...

The purpose of the present invention is to provide a feed additive composition for ruminants the feed additive composition being highly protective in the lumens and exhibiting excellent dissolution in the gastrointestinal tract. The present invention pertains to a feed additive composition for ruminants containing (...

The purpose of the present invention is to develop a new technique for improving secretory production of a heterologous protein using a coryneform bacterium and to provide a secretory production method for a heterologous protein. In the present invention a coryneform bacterium that has the ability to perform secreto...

The purpose of the present invention is to provide an additive composition for ruminant feeds, which contains a biologically active substance at a high concentration, is of a dispersed type, can be highly protected in a rumen, and has excellent elution properties in a digestive tract. The present invention relates t...

The present invention provides a coated livestock weight-gain or feed-efficiency-improving agent constituted by: a core that contains a physiologically active substance having gastrointestinal epithelial cell membrane reinforcing properties; and a core coating agent.

The present invention provides a technique that allows fabrication of antibodies, in particular, regioselective fabrication of antibodies. More specifically, the present invention provides a compound, or a salt thereof, comprising: a substance having affinity for an antibody; a cleavage site; and a reactive group. T...

The present invention provides art that enables modification of an antibody, particularly regioselective modification of an antibody. More specifically, the present invention provides a substance having affinity for an antibody, and a compound or salt thereof having a bioorthogonal functional group, which are repres...

Provided is a method capable of manufacturing morpholino oligonucleotides efficiently and with high yields in a liquid phase synthesis method by using, as starting material, a morpholino nucleotide in which the 5'-position hydroxyl group or a hydroxyl group present on a substituent of the 5'-position hydroxyl group ...

A method for the efficient secretory production of a heterologous protein by a coryneform bacterium utilizing the TorA signal peptide is provided through the development of a novel technique for reducing missed cleavages of the TorA signal peptide. The secretory production of a heterologous protein is carried out by...

The present invention provides an alternative production method for ferritin containing an organic compound enclosed therein. Specifically, the present invention provides a method for producing ferritin containing an organic compound enclosed therein, the method including (1) mixing the organic compound with ferrit...

The present invention pertains to: a culture additive for animal cells, said culture additive containing an amino acid and/or glucose; a culture medium for animal cells, said culture medium containing an amino acid and/or glucose in addition to medium component(s); and a culture method for animal cells, said method ...

The present invention pertains to a technique for controlling the bonding ratio of an antibody and a modified group to a desired range. More specifically, the present invention pertains to: a compound represented by formula (I) [in the formula, X represents a leaving group, Y represents an affinity peptide having a ...

Provided are a composition for culturing animal cells and a technology related thereto. The composition for culturing animal cells contains an amino acid source and an a–Ketoglutaric acid and has a property (A) and/or (B). (A): The a–Ketoglutaric acid is a salt of an a–Ketoglutaric acid. (B): The amino acid source i...

The present invention provides a conjugate of an antibody and a functional substance, said conjugate having excellent desired properties, or a salt thereof. More particularly, the present invention provides a conjugate of an antibody and a functional substance, said conjugate containing a structural unit represented...

A technique is provided for improving the flavor of food. The flavor of food is improved by admixing the following component (A). (A) the fraction containing cell walls of gram-positive bacteria.

The present invention provides: a conjugate of a functional substance and an antibody, in which the binding ratio between the antibody and the functional substance is controlled to within a specific range, said conjugate being exceptional with respect to a desired property; or a salt of said conjugate. More specific...

Provided is a method for the secretory production of an unnatural-amino-acid-containing protein. In the present invention, coryneform bacteria that have a genetic construct for secretory expression of an unnatural-amino-acid-containing protein and that have been modified to express an orthogonal pair of tRNA and ami...

The present invention provides a technique enabling modification of an antibody, particularly regioselective modification of an antibody. More specifically, the present invention provides a compound having an affinity substance to an antibody and a bioorthogonal functional group, represented by the following F...

The present invention provides: a conjugate of an antibody and a functional substance, in which the binding ratio between the antibody and the functional substance is adjusted so as to fall within a desired range and which is superior with respect to a desired property; or a salt of the conjugate. More specifically,...

The present invention provides a protected nucleotide for elongation, which can be purified efficiently and in a high yield by a liquid-liquid extraction operation, and can achieve s an oligonucleotide production method by a phosphoramidite method. It has been found that the above-mentioned problem can be solv...

The present invention relates to a technology for facile chemical modification of only one heavy chain in an antibody structural unit (immunoglobulin unit comprising two heavy chains and optionally two light chains). More specifically, the present invention relates to a compound that contains (A) an affinity substan...

Provided is a perfusion culture method, etc. enabling improvement of productivity in the manufacture of recombinant proteins. Specifically, provided is a method, etc. for enhancing production of recombinant proteins in a perfusion culture of animal cells that have a recombinant protein producing capability, the meth...

Provided are a method, an agent, etc. for enhancing the stability of a medium and enhancing the productivity of a recombinant protein. More specifically, provided are: a medium containing N-acetylcysteine and L-cysteine; a method for culturing cells using a medium containing N-acetylcysteine and L-cysteine; a method...

The present invention addresses the problem of developing a novel technology for improving the secretory production of heterologous proteins by coryneform bacteria and providing a method for the secretory production of heterologous proteins. Coryneform bacteria that have been modified such as to be capable of perfor...

The present invention provides a conjugate of an antibody and a functional substance or a salt of said conjugate, said conjugate or salt thereof having excellent desired properties. Specifically, the present invention provides a conjugate of an antibody and a functional substance or a salt of said conjugate, as well...

The present invention provides an antibody-functional substance conjugate, or salt thereof, having excellent desired properties. More specifically, the present invention provides an antibody-functional substance conjugate comprising a structural unit represented by formula (1): [In the formula, Ig represents an immu...

The present invention provides, for example: a culture medium composition for promoting the production of viruses each carrying a target gene in the culturing of cells capable of producing viruses each having the target gene introduced therein, the composition containing a pyruvate dehydrogenase kinase inhibitor; and ...

The present invention provides: a culture medium composition for promoting the production of a virus having a target gene during the culture of mammalian cells that produce the virus having the target gene introduced therein, said culture medium composition containing ß-alanine; and a culture medium composition for cu...