Hepes Containing Medium

Abstract: The purpose of the present invention is to provide a medium for cell culture that permits cell culture without exhibiting toxicity when a high-concentration HEPES buffer is used, a method for producing said medium, and a cell culture method in which the medium is used. A medium for cell culture containing 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) in a concentration of 30 mM or higher and having an osmotic pressure of 220-340 mOsm/kg, and a cell culture method in which the medium is used, make it possible to improve the cell proliferation rate.

Patent Information

Application #

Filing Date

19 October 2022

Publication Number

43/2022

Publication Type

INA

Invention Field

BIOTECHNOLOGY

Status

Email

patent@depenning.com

Parent Application

Applicants

AJINOMOTO CO., INC.

15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1048315

Inventors

1. SUZUKI, Yu

c/o AJINOMOTO CO., INC., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 2108681

2. KITAZAWA, Manabu

c/o AJINOMOTO CO., INC., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 2108681

3. OGAWA, Shimpei

c/o AJINOMOTO CO., INC., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 2108681

Specification

Documents

Application Documents

#	Name	Date
1	202247059694.pdf	2022-10-19
2	202247059694-STATEMENT OF UNDERTAKING (FORM 3) [19-10-2022(online)].pdf	2022-10-19
3	202247059694-PROOF OF RIGHT [19-10-2022(online)].pdf	2022-10-19
4	202247059694-PRIORITY DOCUMENTS [19-10-2022(online)].pdf	2022-10-19
5	202247059694-FORM 1 [19-10-2022(online)].pdf	2022-10-19
6	202247059694-DRAWINGS [19-10-2022(online)].pdf	2022-10-19
7	202247059694-DECLARATION OF INVENTORSHIP (FORM 5) [19-10-2022(online)].pdf	2022-10-19
8	202247059694-COMPLETE SPECIFICATION [19-10-2022(online)].pdf	2022-10-19
9	202247059694-FORM-26 [15-11-2022(online)].pdf	2022-11-15
10	202247059694-Correspondence_Form 1 And Form 26_30-01-2023.pdf	2023-01-30
11	202247059694-FORM 3 [12-04-2023(online)].pdf	2023-04-12
12	202247059694-FORM 18 [20-02-2024(online)].pdf	2024-02-20