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Medium For Culturing I Ps Cell
Abstract: The purpose of the present invention is to provide a medium for propagation of stem cells with good performance that reduces the use of albumin while maintaining medium performance stability. The present invention provides a medium for iPS cell propagation characterized by including a water soluble polymer such as polyvinyl alcohol with albumin.
Notices, Deadlines & Correspondence
Patent Information
Applicants
AJINOMOTO CO., INC.
Inventors
1. KURIYAMA, Yoko
2. OKAMOTO Satoru
3. KITAZAWA Manabu
4. SUGIMOTO Nao
5. MATSUMOTO Takuya
Specification
Claims
1. A medium for culturing an iPS cell, comprising a water-soluble polymer and an albumin.
2. The medium according to claim 1, wherein the water-soluble polymer is polyvinyl alcohol.
3. The medium according to claim 2, wherein the polyvinyl alcohol has a critical micelle concentration of 0.1 - 5 mg/ml.
4. The medium according to claim 2 or 3, wherein the polyvinyl alcohol has a hydrolysis ratio of 60 - 95%.
5. The medium according to any one of claims 1 to 4, wherein :he albumin carries a reduced amount of fatty acid.
5. A medium additive comprising a water-soluble polymer as an active ingredient.
/. The agent according to claim 6, wherein the water-soluble polymer is polyvinyl alcohol.
3. The agent according to claim 7, wherein the polyvinyl alcohol has a critical micelle concentration of 0.1 - 5 mg/ml.
}. The agent according to claim 7 or 8, wherein the polyvinyl alcohol has a hydrolysis ratio of 60 - 95%.
L0. The agent according to any one of claims 6 to 9, wherein ;he medium is an albumin-containing medium.
ul. The agent according to claim 10, wherein the albumin carries a reduced amount of fatty acid.
12. The agent according to any one of claims 6 to 11, wherein the medium is a medium for proliferation of stem cells.
13. The agent according to claim 12, wherein the stem cell is an iPS cell.
14. The agent according to any one of claims 6 to 13, which is an agent for prevention of degradation of an albumin-containing medium.
15. A method of preventing degradation of an albumin-containing medium, comprising adding a water-soluble polymer.
16. The method according to claim 15, wherein the water-soluble polymer is polyvinyl alcohol.
17. A method of culturing an iPS cell, comprising culturing in the culture medium according to any one of claims 1 to 5.
Documents
Orders
| Section | Controller | Decision Date |
|---|---|---|
Application Documents
| # | Name | Date |
|---|---|---|
| 1 | PROOF OF RIGHT [31-10-2016(online)].pdf | 2016-10-31 |
| 2 | Power of Attorney [31-10-2016(online)].pdf | 2016-10-31 |
| 3 | Form 5 [31-10-2016(online)].pdf | 2016-10-31 |
| 4 | Form 3 [31-10-2016(online)].pdf | 2016-10-31 |
| 5 | Form 18 [31-10-2016(online)].pdf_63.pdf | 2016-10-31 |
| 6 | Form 18 [31-10-2016(online)].pdf | 2016-10-31 |
| 7 | Form 1 [31-10-2016(online)].pdf | 2016-10-31 |
| 8 | Drawing [31-10-2016(online)].pdf | 2016-10-31 |
| 9 | Description(Complete) [31-10-2016(online)].pdf | 2016-10-31 |
| 10 | 201647037214 IB304 31-10-2016.pdf | 2016-10-31 |
| 11 | 201647037214 Form-2 31-10-2016.pdf | 2016-10-31 |
| 12 | 201647037214 English Translation 31-10-2016.pdf | 2016-10-31 |
| 13 | 201647037214 Description Complete 31-10-2016.pdf | 2016-10-31 |
| 14 | 201647037214 Correspondence 31-10-2016.pdf | 2016-10-31 |
| 15 | 201647037214 Claims 31-10-2016.pdf | 2016-10-31 |
| 16 | 201647037214 Abstract 31-10-2016.pdf | 2016-10-31 |
| 17 | 201647037214.pdf | 2016-11-02 |
| 18 | 201647037214-FORM-1-081116.pdf | 2016-11-14 |
| 19 | Form 3 [17-04-2017(online)].pdf | 2017-04-17 |
| 20 | 201647037214-FORM 3 [15-02-2018(online)].pdf | 2018-02-15 |
| 21 | 201647037214-FER.pdf | 2019-05-07 |
| 22 | 201647037214-OTHERS [05-11-2019(online)].pdf | 2019-11-05 |
| 23 | 201647037214-Information under section 8(2) (MANDATORY) [05-11-2019(online)].pdf | 2019-11-05 |
| 24 | 201647037214-FORM-26 [05-11-2019(online)].pdf | 2019-11-05 |
| 25 | 201647037214-FORM 3 [05-11-2019(online)].pdf | 2019-11-05 |
| 26 | 201647037214-FER_SER_REPLY [05-11-2019(online)].pdf | 2019-11-05 |
| 27 | 201647037214-DRAWING [05-11-2019(online)].pdf | 2019-11-05 |
| 28 | 201647037214-COMPLETE SPECIFICATION [05-11-2019(online)].pdf | 2019-11-05 |
| 29 | 201647037214-CLAIMS [05-11-2019(online)].pdf | 2019-11-05 |
| 30 | 201647037214-ABSTRACT [05-11-2019(online)].pdf | 2019-11-05 |
| 31 | Correspondence by Agent_Form26_08-11-2019.pdf | 2019-11-08 |
| 32 | 201647037214-Information under section 8(2) (MANDATORY) [03-01-2020(online)].pdf | 2020-01-03 |
| 33 | 201647037214-FORM 3 [03-01-2020(online)].pdf | 2020-01-03 |
| 34 | 201647037214-US(14)-HearingNotice-(HearingDate-21-07-2020).pdf | 2020-06-22 |
| 35 | 201647037214-US(14)-ExtendedHearingNotice-(HearingDate-21-08-2020).pdf | 2020-07-17 |
| 36 | 201647037214-REQUEST FOR ADJOURNMENT OF HEARING UNDER RULE 129A [17-07-2020(online)].pdf | 2020-07-17 |
| 37 | 201647037214-Correspondence to notify the Controller [18-08-2020(online)].pdf | 2020-08-18 |
| 38 | 201647037214-Written submissions and relevant documents [07-09-2020(online)].pdf | 2020-09-07 |
| 39 | 201647037214-Retyped Pages under Rule 14(1) [07-09-2020(online)].pdf | 2020-09-07 |
| 40 | 201647037214-Information under section 8(2) [07-09-2020(online)].pdf | 2020-09-07 |
| 41 | 201647037214-FORM 3 [07-09-2020(online)].pdf | 2020-09-07 |
| 42 | 201647037214-2. Marked Copy under Rule 14(2) [07-09-2020(online)].pdf | 2020-09-07 |
| 43 | 201647037214-PatentCertificate24-09-2021.pdf | 2021-09-24 |
| 44 | 201647037214-IntimationOfGrant24-09-2021.pdf | 2021-09-24 |
| 45 | 201647037214-RELEVANT DOCUMENTS [11-09-2023(online)].pdf | 2023-09-11 |
Search Strategy
| 1 | 201647037214_07-05-2019.pdf |