View All Documents & Correspondence
Medium For Neural Stem Cells Enhancing Neural Differentiation Ability
Abstract: The present invention provides a medium for neural stem cells and/or neural progenitor cells and substantially free of one or more amino acids selected from the group consisting of L-glutamine, L-asparagine and L-aspartic acid, and a method for culturing the neural stem cells and/or neural progenitor cells in the medium, and the like. [Figure 1]
Notices, Deadlines & Correspondence
Patent Information
Applicants
AJINOMOTO CO., INC.
Inventors
1. MATSUMOTO, Takuya
2. HARATA, Ikue
3. SENDA, Sho
Claims
1. A medium substantially free of L-glutamine, for culturing neural stem cells and/or neural progenitor cells.
2. The medium according to claim 1, having an L-glutamine concentration of not more than 100 uM.
3. The medium according to claim 1 or 2, not comprising L-glutamine.
4. The medium according to any one of claims 1 to 3, comprising holotransferrin.
5. The medium according to any one of claims 1 to 4, comprising L-tryptophan, L-lysine, L-methionine, L-phenylalanine, L-threonine, L-valine, L-leucine, L-isoleucine and L-histidine.
6. The medium according to any one of claims 1 to 5 for promoting the neuronal differentiation potency of a neural stem cell and/or a neural progenitor cell.
7. The medium according to any one of claims 1 to 6 that is a serum-free medium.
8. A method for culturing a neural stem cell and/or a neural progenitor cell, comprising culturing the cell(s) in the medium according to any one of claims 1 to 7.
9. The method according to claim 8 for producing a neural stem cell and/or a neural progenitor cell with promoted neuronal differentiation potency.
10. The method according to claim 8 or 9, wherein the culturing is performed for a period of not less than 4 days.
11. A method for producing a nerve cell, comprising the following steps: (I) culturing a neural stem cell and/or a neural progenitor cell in the medium according to any one of claims 1 to 7 to give the neural stem cell and/or the neural progenitor cell with promoted neuronal differentiation potency; (II) culturing the neural stem cell and/or the neural progenitor cell with promoted neuronal differentiation potency and obtained in step (I) under nerve cell differentiation induction conditions to give a nerve cell population.
12. A neural stem cell and/or a neural progenitor cell with promoted neuronal differentiation potency and obtained by the method according to claim 9.
13. A culture preparation comprising a neural stem cell and/or a neural progenitor cell, and the medium according to any one of claims 1 to 7.
Specification
WE CLAIM:
1. A medium substantially free of L-glutamine, for culturing neural stem cells and/or neural progenitor cells.
2. The medium according to claim 1, having an L-glutamine concentration of not more than 100 uM.
3. The medium according to claim 1 or 2, not comprising L-glutamine.
4. The medium according to any one of claims 1 to 3, comprising holotransferrin.
5. The medium according to any one of claims 1 to 4, comprising L-tryptophan, L-lysine, L-methionine, L-phenylalanine, L-threonine, L-valine, L-leucine, L-isoleucine and L-histidine.
6. The medium according to any one of claims 1 to 5 for promoting the neuronal differentiation potency of a neural stem cell and/or a neural progenitor cell.
7. The medium according to any one of claims 1 to 6 that is a serum-free medium.
8. A method for culturing a neural stem cell and/or a neural progenitor cell, comprising culturing the cell(s) in the medium according to any one of claims 1 to 7.
9. The method according to claim 8 for producing a neural stem cell and/or a neural progenitor cell with promoted neuronal differentiation potency.
10. The method according to claim 8 or 9, wherein the culturing
is performed for a period of not less than 4 days.
11. A method for producing a nerve cell, comprising the
following steps:
(I) culturing a neural stem cell and/or a neural progenitor
cell in the medium according to any one of claims 1 to 7 to
give the neural stem cell and/or the neural progenitor cell
with promoted neuronal differentiation potency;
(II) culturing the neural stem cell and/or the neural
progenitor cell with promoted neuronal differentiation potency
and obtained in step (I) under nerve cell differentiation
induction conditions to give a nerve cell population.
12. A neural stem cell and/or a neural progenitor cell with promoted neuronal differentiation potency and obtained by the method according to claim 9.
13. A culture preparation comprising a neural stem cell and/or a neural progenitor cell, and the medium according to any one of claims 1 to 7.