"Method For Amplifying And/Or Detecting Nucleic Acids, Kits And Uses Of Said Method"

Abstract: The present invention relates to a method of amplification and/or of detection for removing contaminants in a liquid biological sample containing nucleic acids of interest that we wish to amplify, said method comprising the following stages: a) treating the biological sample chemically or enzymatically to permit conversion of one type of base of said nucleic acids of interest to another type of base; b) adding amplification primers, intended for specifically amplifying said converted nucleic acids of interest, each primer being constituted of three different types of bases; c) adding to the biological sample, after these treatments, the reagents necessary for the amplification, such as aqueous solution(s), solvent(s), nucleotides, enzyme(s), but also said primers previously synthesized; d) placing said solution and the reagents in conditions permitting amplification and/or detection of the converted nucleic acids. The invention also relates to a kit for applying said method, as well as to a use of said method or the kit for specifically amplifying and detecting bacterial, eubacterial, fungal, pan-fungal, viral or yeast targets. The invention finds application preferably in the area of diagnostics.

Patent Information

Applicants

Inventors

Specification

Documents

Application Documents

Search Strategy