"Method For Isolating Or Counting Microorganisms On An Agar Culture Medium"

Abstract: The invention relates to a method for isolating microorganisms on an agar culture medium, including the steps of: depositing, on said agar culture medium, a predetermined amount of a sample to be analyzed and optionally containing said microorganisms or a suspension of said microorganisms; applying a seeding means onto agar culture medium in contact with the volume of sample or suspension; moving the seeding means so as totally or partially spread the volume of sample or suspension over the surface of the agar culture medium, the movement of said seeding means being discontinuous so that during said movement the contact between the seeding means and the surface of the agar culture medium is interrupted and re-established at least once, thereby creating spreading segments and resulting in a depletion of the seeding means in terms of the sample or suspension; and incubating said agar culture medium under conditions enabling the growth of microorganisms. Figure 2

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