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Method For Preparing Broussonetia Kazinoki Extract

Abstract: The present invention provides a method for preparing a Broussonetia kazinoki extract  comprising the following steps: solvent extracting Broussonetia kazinoki; isolating the Broussonetia kazinoki extract  extracted in the previous step; and crystallizing the Broussonetia kazinoki extract isolated in the previous step. It is possible to prepare a Broussonetia kazinoki extract having a remarkable skin whitening effect and excellent stability through the method for preparing a Broussonetia kazinoki extract.

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Notices, Deadlines & Correspondence

Patent Information

Application #
Filing Date
12 December 2012
Publication Number
52/2015
Publication Type
INA
Invention Field
PHARMACEUTICALS
Status
Email
Parent Application
Patent Number
Legal Status
Grant Date
2019-06-24
Renewal Date

Applicants

AMOREPACIFIC CORPORATION
181  2-ga  Hangang-ro  Yongsan-gu  Seoul  140-777  Republic of Korea.

Inventors

1. LIM  Hyung Jun
#101-502  Brown Stone Bangbae Apt.  899-16  Bangbae 1-dong  Seocho-gu  Seoul  137-841  Republic of Korea.
2. LEE  Jin Young
Amorepacific R & D Center  314-1  Bora-dong  Giheung-gu  Yongin-si  Gyeonggi-do  446-729  Republic of Korea.
3. PARK  Sun Young
Amorepacific R & D Center  314-1  Bora-dong  Giheung-gu  Yongin-si  Gyeonggi-do  446-729  Republic of Korea.
4. AN  Soon Ae
#308-903  Geumhwamaeul Jugong Greenvill Apt.  Sanggal-dong  Giheung-gu  Yongin-si  Gyeonggi-do  446-760  Republic of Korea.
5. LEE  Chan Woo
Amorepacific R & D Center  314-1  Bora-dong  Giheung-gu  Yongin-si  Gyeonggi-do  446-729  Republic of Korea.
6. KIM  Eun Joo
Amorepacific R & D Center  314-1  Bora-dong  Giheung-gu  Yongin-si  Gyeonggi-do  446-729  Republic of Korea.
7. KIM  Jun Oh
Amorepacific R & D Center  314-1  Bora-dong  Giheung-gu  Yongin-si  Gyeonggi-do  446-729  Republic of Korea.
8. HAN  Sang Hun
#2-203  Cheonnok Apt.  273-3  Yuljeon-dong  Jangan-gu  Suwon-si  Gyeonggi-do  440-825  Republic of Korea.
9. KIM  Han Kon
#201-903  Jugong Greenvill Apt.  1260  Maetan-dong  Yeongtong-gu  Suwon-si  Gyeonggi-do  443-793  Republic of Korea.

Specification

1. A method for preparing a composition comprising a carrier; and a crystallized Broussonetia
kazinoki extract loaded onto the carrier, comprising the steps of:
(1) extracting Broussonetia kazinoki with solvent;
(2) allowing the Broussonetia kazinoki extract resulting from step (1) to stand;
(3) crystallizing the Broussonetia kazinoki extract resulting from step (2); and
(4) loading the crystallized Broussonetia kazinoki extract onto a carrier, wherein the carrier comprises a mixture of polyethelenadipate (PEA) and polymethlmethacrylate (PMMA).

2. The method as claimed in claim 1, wherein the Broussonetia kazinoki includes the root bark of Broussonetia kazinoki.
3. The method as claimed in claim 1, wherein the solvent includes alcohol.
4. The method as claimed in claim 1, wherein the extraction is performed at a temperature between 50 to 100 °C for 1-10 hours.
5. The method as claimed in claim 1, wherein the step of allowing the Broussonetia kazinoki extract to stand is performed at a temperature between -5 and 25 °C for 1-40 hours.
6. The method as claimed in claim 1, wherein the step of crystallizing the Broussonetia kazinoki extract comprises stirring the Broussonetia kazinoki extract in water at a temperature between -5 and
15 °C for 1-20 hours.
7. The method as claimed in claim 1, wherein the crystallized Broussonetia kazinoki extract is used in an amount of 0.01-30 wt% based on the weight of the carrier.
8. The method as claimed in claim 1, wherein the step (3) comprises obtaining the skin whitening active ingredient from the crystallized Broussonetia kazinoki extract by HSCCC (high-speed counter current chromatography) using a two-phase solvent system.
9. The method as claimed in claim 8, wherein the skin whitening active ingredient includes kazinol C.
10. The method as claimed in claim 8, wherein the two-phase solvent system includes hexane, ethyl acetate, methanol and water at a ratio of 1 -10 :1 -10 :1 -10 :1 -10.
11. A composition comprising a carrier; and a crystallized Broussonetia kazinoki extract loaded onto the carrier for skin whitening, wherein the composition prepared by the method of any one of
rlaimc 1 tn 1 fl

12. The composition as claimed in claim 11, wherein the composition has effects of inhibiting melanin production and tyrosinase.

Documents

Orders

Section Controller Decision Date

Application Documents

# Name Date
1 10384-CHENP-2012-RELEVANT DOCUMENTS [21-08-2023(online)].pdf 2023-08-21
1 Priority Document.pdf 2012-12-14
2 10384-CHENP-2012-RELEVANT DOCUMENTS [24-09-2022(online)].pdf 2022-09-24
2 Power of Authority.pdf 2012-12-14
3 Form-5.pdf 2012-12-14
3 10384-CHENP-2012-RELEVANT DOCUMENTS [27-09-2021(online)].pdf 2021-09-27
4 Form-3.pdf 2012-12-14
4 10384-CHENP-2012-RELEVANT DOCUMENTS [30-08-2021(online)].pdf 2021-08-30
5 Form-1.pdf 2012-12-14
5 10384-CHENP-2012-RELEVANT DOCUMENTS [21-02-2020(online)].pdf 2020-02-21
6 Drawings.pdf 2012-12-14
6 10384-CHENP-2012-IntimationOfGrant24-06-2019.pdf 2019-06-24
7 10384-CHENP-2012-PatentCertificate24-06-2019.pdf 2019-06-24
7 10384-CHENP-2012 FORM-3 05-04-2013.pdf 2013-04-05
8 Abstract_Granted 314553_24-06-2019.pdf 2019-06-24
8 10384-CHENP-2012 FORM-13 05-04-2013.pdf 2013-04-05
9 10384-CHENP-2012 FORM-1 05-04-2013.pdf 2013-04-05
9 Claims_Granted 314553_24-06-2019.pdf 2019-06-24
10 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf 2013-04-05
10 Description_Granted 314553_24-06-2019.pdf 2019-06-24
11 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf 2013-04-05
11 Drawings_Granted 314553_24-06-2019.pdf 2019-06-24
12 Form 3 [12-09-2016(online)].pdf 2016-09-12
12 Marked Up Claims_Granted 314553_24-06-2019.pdf 2019-06-24
13 10384-CHENP-2012-Written submissions and relevant documents (MANDATORY) [21-06-2019(online)].pdf 2019-06-21
13 Form 3 [09-03-2017(online)].pdf 2017-03-09
14 10384-CHENP-2012-FORM 3 [07-03-2018(online)].pdf 2018-03-07
14 10384-CHENP-2012-HearingNoticeLetter.pdf 2019-05-06
15 10384-CHENP-2012-FER.pdf 2018-04-11
15 Correspondence by Agent_General Power of Attorney_22-10-2018.pdf 2018-10-22
16 10384-CHENP-2012-CLAIMS [11-10-2018(online)].pdf 2018-10-11
16 10384-CHENP-2012-FORM 4(ii) [29-09-2018(online)].pdf 2018-09-29
17 10384-CHENP-2012-PETITION UNDER RULE 137 [11-10-2018(online)].pdf 2018-10-11
17 10384-CHENP-2012-COMPLETE SPECIFICATION [11-10-2018(online)].pdf 2018-10-11
18 10384-CHENP-2012-CORRESPONDENCE [11-10-2018(online)].pdf 2018-10-11
18 10384-CHENP-2012-OTHERS [11-10-2018(online)].pdf 2018-10-11
19 10384-CHENP-2012-FER_SER_REPLY [11-10-2018(online)].pdf 2018-10-11
19 10384-CHENP-2012-FORM-26 [11-10-2018(online)].pdf 2018-10-11
20 10384-CHENP-2012-FER_SER_REPLY [11-10-2018(online)].pdf 2018-10-11
20 10384-CHENP-2012-FORM-26 [11-10-2018(online)].pdf 2018-10-11
21 10384-CHENP-2012-CORRESPONDENCE [11-10-2018(online)].pdf 2018-10-11
21 10384-CHENP-2012-OTHERS [11-10-2018(online)].pdf 2018-10-11
22 10384-CHENP-2012-COMPLETE SPECIFICATION [11-10-2018(online)].pdf 2018-10-11
22 10384-CHENP-2012-PETITION UNDER RULE 137 [11-10-2018(online)].pdf 2018-10-11
23 10384-CHENP-2012-CLAIMS [11-10-2018(online)].pdf 2018-10-11
23 10384-CHENP-2012-FORM 4(ii) [29-09-2018(online)].pdf 2018-09-29
24 Correspondence by Agent_General Power of Attorney_22-10-2018.pdf 2018-10-22
24 10384-CHENP-2012-FER.pdf 2018-04-11
25 10384-CHENP-2012-FORM 3 [07-03-2018(online)].pdf 2018-03-07
25 10384-CHENP-2012-HearingNoticeLetter.pdf 2019-05-06
26 10384-CHENP-2012-Written submissions and relevant documents (MANDATORY) [21-06-2019(online)].pdf 2019-06-21
26 Form 3 [09-03-2017(online)].pdf 2017-03-09
27 Form 3 [12-09-2016(online)].pdf 2016-09-12
27 Marked Up Claims_Granted 314553_24-06-2019.pdf 2019-06-24
28 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf 2013-04-05
28 Drawings_Granted 314553_24-06-2019.pdf 2019-06-24
29 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf 2013-04-05
29 Description_Granted 314553_24-06-2019.pdf 2019-06-24
30 10384-CHENP-2012 FORM-1 05-04-2013.pdf 2013-04-05
30 Claims_Granted 314553_24-06-2019.pdf 2019-06-24
31 Abstract_Granted 314553_24-06-2019.pdf 2019-06-24
31 10384-CHENP-2012 FORM-13 05-04-2013.pdf 2013-04-05
32 10384-CHENP-2012-PatentCertificate24-06-2019.pdf 2019-06-24
32 10384-CHENP-2012 FORM-3 05-04-2013.pdf 2013-04-05
33 Drawings.pdf 2012-12-14
33 10384-CHENP-2012-IntimationOfGrant24-06-2019.pdf 2019-06-24
34 Form-1.pdf 2012-12-14
34 10384-CHENP-2012-RELEVANT DOCUMENTS [21-02-2020(online)].pdf 2020-02-21
35 Form-3.pdf 2012-12-14
35 10384-CHENP-2012-RELEVANT DOCUMENTS [30-08-2021(online)].pdf 2021-08-30
36 Form-5.pdf 2012-12-14
36 10384-CHENP-2012-RELEVANT DOCUMENTS [27-09-2021(online)].pdf 2021-09-27
37 10384-CHENP-2012-RELEVANT DOCUMENTS [24-09-2022(online)].pdf 2022-09-24
37 Power of Authority.pdf 2012-12-14
38 10384-CHENP-2012-RELEVANT DOCUMENTS [21-08-2023(online)].pdf 2023-08-21
38 Priority Document.pdf 2012-12-14

Search Strategy

1 10384_chenp_2012_05-04-2018.pdf

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