Abstract: The present invention provides a method for preparing a Broussonetia kazinoki extract comprising the following steps: solvent extracting Broussonetia kazinoki; isolating the Broussonetia kazinoki extract extracted in the previous step; and crystallizing the Broussonetia kazinoki extract isolated in the previous step. It is possible to prepare a Broussonetia kazinoki extract having a remarkable skin whitening effect and excellent stability through the method for preparing a Broussonetia kazinoki extract.
1. A method for preparing a composition comprising a carrier; and a crystallized Broussonetia
kazinoki extract loaded onto the carrier, comprising the steps of:
(1) extracting Broussonetia kazinoki with solvent;
(2) allowing the Broussonetia kazinoki extract resulting from step (1) to stand;
(3) crystallizing the Broussonetia kazinoki extract resulting from step (2); and
(4) loading the crystallized Broussonetia kazinoki extract onto a carrier, wherein the carrier comprises a mixture of polyethelenadipate (PEA) and polymethlmethacrylate (PMMA).
2. The method as claimed in claim 1, wherein the Broussonetia kazinoki includes the root bark of Broussonetia kazinoki.
3. The method as claimed in claim 1, wherein the solvent includes alcohol.
4. The method as claimed in claim 1, wherein the extraction is performed at a temperature between 50 to 100 °C for 1-10 hours.
5. The method as claimed in claim 1, wherein the step of allowing the Broussonetia kazinoki extract to stand is performed at a temperature between -5 and 25 °C for 1-40 hours.
6. The method as claimed in claim 1, wherein the step of crystallizing the Broussonetia kazinoki extract comprises stirring the Broussonetia kazinoki extract in water at a temperature between -5 and
15 °C for 1-20 hours.
7. The method as claimed in claim 1, wherein the crystallized Broussonetia kazinoki extract is used in an amount of 0.01-30 wt% based on the weight of the carrier.
8. The method as claimed in claim 1, wherein the step (3) comprises obtaining the skin whitening active ingredient from the crystallized Broussonetia kazinoki extract by HSCCC (high-speed counter current chromatography) using a two-phase solvent system.
9. The method as claimed in claim 8, wherein the skin whitening active ingredient includes kazinol C.
10. The method as claimed in claim 8, wherein the two-phase solvent system includes hexane, ethyl acetate, methanol and water at a ratio of 1 -10 :1 -10 :1 -10 :1 -10.
11. A composition comprising a carrier; and a crystallized Broussonetia kazinoki extract loaded onto the carrier for skin whitening, wherein the composition prepared by the method of any one of
rlaimc 1 tn 1 fl
12. The composition as claimed in claim 11, wherein the composition has effects of inhibiting melanin production and tyrosinase.
| Section | Controller | Decision Date |
|---|---|---|
| # | Name | Date |
|---|---|---|
| 1 | 10384-CHENP-2012-RELEVANT DOCUMENTS [21-08-2023(online)].pdf | 2023-08-21 |
| 1 | Priority Document.pdf | 2012-12-14 |
| 2 | 10384-CHENP-2012-RELEVANT DOCUMENTS [24-09-2022(online)].pdf | 2022-09-24 |
| 2 | Power of Authority.pdf | 2012-12-14 |
| 3 | Form-5.pdf | 2012-12-14 |
| 3 | 10384-CHENP-2012-RELEVANT DOCUMENTS [27-09-2021(online)].pdf | 2021-09-27 |
| 4 | Form-3.pdf | 2012-12-14 |
| 4 | 10384-CHENP-2012-RELEVANT DOCUMENTS [30-08-2021(online)].pdf | 2021-08-30 |
| 5 | Form-1.pdf | 2012-12-14 |
| 5 | 10384-CHENP-2012-RELEVANT DOCUMENTS [21-02-2020(online)].pdf | 2020-02-21 |
| 6 | Drawings.pdf | 2012-12-14 |
| 6 | 10384-CHENP-2012-IntimationOfGrant24-06-2019.pdf | 2019-06-24 |
| 7 | 10384-CHENP-2012-PatentCertificate24-06-2019.pdf | 2019-06-24 |
| 7 | 10384-CHENP-2012 FORM-3 05-04-2013.pdf | 2013-04-05 |
| 8 | Abstract_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 8 | 10384-CHENP-2012 FORM-13 05-04-2013.pdf | 2013-04-05 |
| 9 | 10384-CHENP-2012 FORM-1 05-04-2013.pdf | 2013-04-05 |
| 9 | Claims_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 10 | 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf | 2013-04-05 |
| 10 | Description_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 11 | 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf | 2013-04-05 |
| 11 | Drawings_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 12 | Form 3 [12-09-2016(online)].pdf | 2016-09-12 |
| 12 | Marked Up Claims_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 13 | 10384-CHENP-2012-Written submissions and relevant documents (MANDATORY) [21-06-2019(online)].pdf | 2019-06-21 |
| 13 | Form 3 [09-03-2017(online)].pdf | 2017-03-09 |
| 14 | 10384-CHENP-2012-FORM 3 [07-03-2018(online)].pdf | 2018-03-07 |
| 14 | 10384-CHENP-2012-HearingNoticeLetter.pdf | 2019-05-06 |
| 15 | 10384-CHENP-2012-FER.pdf | 2018-04-11 |
| 15 | Correspondence by Agent_General Power of Attorney_22-10-2018.pdf | 2018-10-22 |
| 16 | 10384-CHENP-2012-CLAIMS [11-10-2018(online)].pdf | 2018-10-11 |
| 16 | 10384-CHENP-2012-FORM 4(ii) [29-09-2018(online)].pdf | 2018-09-29 |
| 17 | 10384-CHENP-2012-PETITION UNDER RULE 137 [11-10-2018(online)].pdf | 2018-10-11 |
| 17 | 10384-CHENP-2012-COMPLETE SPECIFICATION [11-10-2018(online)].pdf | 2018-10-11 |
| 18 | 10384-CHENP-2012-CORRESPONDENCE [11-10-2018(online)].pdf | 2018-10-11 |
| 18 | 10384-CHENP-2012-OTHERS [11-10-2018(online)].pdf | 2018-10-11 |
| 19 | 10384-CHENP-2012-FER_SER_REPLY [11-10-2018(online)].pdf | 2018-10-11 |
| 19 | 10384-CHENP-2012-FORM-26 [11-10-2018(online)].pdf | 2018-10-11 |
| 20 | 10384-CHENP-2012-FER_SER_REPLY [11-10-2018(online)].pdf | 2018-10-11 |
| 20 | 10384-CHENP-2012-FORM-26 [11-10-2018(online)].pdf | 2018-10-11 |
| 21 | 10384-CHENP-2012-CORRESPONDENCE [11-10-2018(online)].pdf | 2018-10-11 |
| 21 | 10384-CHENP-2012-OTHERS [11-10-2018(online)].pdf | 2018-10-11 |
| 22 | 10384-CHENP-2012-COMPLETE SPECIFICATION [11-10-2018(online)].pdf | 2018-10-11 |
| 22 | 10384-CHENP-2012-PETITION UNDER RULE 137 [11-10-2018(online)].pdf | 2018-10-11 |
| 23 | 10384-CHENP-2012-CLAIMS [11-10-2018(online)].pdf | 2018-10-11 |
| 23 | 10384-CHENP-2012-FORM 4(ii) [29-09-2018(online)].pdf | 2018-09-29 |
| 24 | Correspondence by Agent_General Power of Attorney_22-10-2018.pdf | 2018-10-22 |
| 24 | 10384-CHENP-2012-FER.pdf | 2018-04-11 |
| 25 | 10384-CHENP-2012-FORM 3 [07-03-2018(online)].pdf | 2018-03-07 |
| 25 | 10384-CHENP-2012-HearingNoticeLetter.pdf | 2019-05-06 |
| 26 | 10384-CHENP-2012-Written submissions and relevant documents (MANDATORY) [21-06-2019(online)].pdf | 2019-06-21 |
| 26 | Form 3 [09-03-2017(online)].pdf | 2017-03-09 |
| 27 | Form 3 [12-09-2016(online)].pdf | 2016-09-12 |
| 27 | Marked Up Claims_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 28 | 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf | 2013-04-05 |
| 28 | Drawings_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 29 | 10384-CHENP-2012 CORRESPONDENCE OTHERS 05-04-2013.pdf | 2013-04-05 |
| 29 | Description_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 30 | 10384-CHENP-2012 FORM-1 05-04-2013.pdf | 2013-04-05 |
| 30 | Claims_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 31 | Abstract_Granted 314553_24-06-2019.pdf | 2019-06-24 |
| 31 | 10384-CHENP-2012 FORM-13 05-04-2013.pdf | 2013-04-05 |
| 32 | 10384-CHENP-2012-PatentCertificate24-06-2019.pdf | 2019-06-24 |
| 32 | 10384-CHENP-2012 FORM-3 05-04-2013.pdf | 2013-04-05 |
| 33 | Drawings.pdf | 2012-12-14 |
| 33 | 10384-CHENP-2012-IntimationOfGrant24-06-2019.pdf | 2019-06-24 |
| 34 | Form-1.pdf | 2012-12-14 |
| 34 | 10384-CHENP-2012-RELEVANT DOCUMENTS [21-02-2020(online)].pdf | 2020-02-21 |
| 35 | Form-3.pdf | 2012-12-14 |
| 35 | 10384-CHENP-2012-RELEVANT DOCUMENTS [30-08-2021(online)].pdf | 2021-08-30 |
| 36 | Form-5.pdf | 2012-12-14 |
| 36 | 10384-CHENP-2012-RELEVANT DOCUMENTS [27-09-2021(online)].pdf | 2021-09-27 |
| 37 | 10384-CHENP-2012-RELEVANT DOCUMENTS [24-09-2022(online)].pdf | 2022-09-24 |
| 37 | Power of Authority.pdf | 2012-12-14 |
| 38 | 10384-CHENP-2012-RELEVANT DOCUMENTS [21-08-2023(online)].pdf | 2023-08-21 |
| 38 | Priority Document.pdf | 2012-12-14 |
| 1 | 10384_chenp_2012_05-04-2018.pdf |