A simple and scaleable process is provided for obtaining recombinant human GM-CSF expressed in microbial cells, secreting mature GM-CSF, culturing these cells and purifying the protein in good yield and of a therapeutic grade quality. The steps include cloning, fermentation and purification of the protein. The purif...

Humanized monoclonal antibodies against human epidermal growth factor receptor, use and method thereof Humanized MAbs are designed, created and produced using a series of innovative processes comprising of computational analysis, modeling studies, rationale assessment and selection of humanized sequences, an efficient...

The present invention discloses a process for stable, liquid therapeutic formulation of G-CSF, wherein the protein is stored in a buffer of conductivity greater than 1.0 milliSiemens/cm and in the pH range of 4.0 to 5.0, in the presence of a surfactant, a tonicity modifier and an amino acid that suppresses the oxidati...

The present invention provides a method for achieving high-level expression of the therapeutically important Iymphokine (human IL-2). The method comprises of identifying the secondary structure in the 5' region of human IL-2 mRNA, modifying the 5' region of the human IL-2 DNA sequence to produce a new DNA sequence whe...

The present invention describes a novel process for large scale purification of therapeutic grade quality of recombinant human G-CSF from microbial cells, wherein the protein is expressed as inclusion bodies. The process involves the novel use of Hydrophobic Interaction Chromatography (HIC) step to purify G-CSF eluted...