The present invention discloses isolation of Penicillin Acylase (PA) from Achromobacter sp CCM 4824 expressed in recombinant strain E. coli BL21 CCM 7394 bearing the recombinant plasmid pKXIPl and processing of PA into biocatalyst useful for the industrial synthesis of antibiotics. More particularly the invention di...

ABSTRACT: The invention discloses an improved cost-effective process for preparation of 7-Dehydrocholesterol of formula I with good yield and purity.

“A Nucleotide Sequence Encoding 7-Dehydrocholesterol Reductase, Recombinant Constructs And Recombinant Organisms Comprising Said Sequence” ABSTRACT: The present invneiton provides a novel nucleotide sequence represented by Seq ID No.1 encoding 7-dehydrocholesterol reductase (7-DHCR), recombinant DNA constructs and r...

The present invention provides a stable and viable biocatalyst with high activity and operational stability and method of immobilization thereof. The immobilization process is based on the principle of entrapment of partially purified enzyme precipitate which is simultaneously aggregated by cross linking agent like gl...

Disclosed herein macroporous polyvinyl acetate copolymer beads directly used to immobilize the lipase without any prior functional modification of beads and also discloses the process for preparation of said copolymer beads and the process of immobilizing the enzyme lipase.

Disclosed herein is acrylonitrile based macroporous terpolymer beads, for immobilization of enzymes to form immobilized enzyme catalyst and its subsequent application in enzymatic synthesis of semi-synthetic β-lactam antibiotics.

This invention discloses stable, highly porous and oxirane macro-porous beads with high enzyme expression abilities based on Glycidyl methacrylate- Ethylene glycol dimethacrylate-Divinyl benzene co-polymers and a novel process for the preparation of the said macro-porous polymer beads involving suspension polymerizati...

The invention consists in a nucleotide sequence having the size of (2646) bp, wherein the order of nucleotides is identical to the order of the nucleotide sequence encoding penicillin acylase from Achromobacter sp. CCM 4824 (formerly Comamonas testosteroni CCM 4824), eventually of the fragments of this sequence havi...

Disclosed herein is a process for synthesis of Molnupiravir from cytidine of using a lipase for esterification reaction. The process comprises of reacting compound of formula II with acetone in presence of sulphuric acid to obtain compound of formula III; enzymatically converting compound of Formula III to compound ...

The present invention discloses an improved catalytic process for the synthesis of 5-(3-pyridyl)-2, 2’-bithiophene in high yield and purity.

The present invention discloses novel method for synthesizing vegan 25-OH cholesterol/Calcifediol from inexpensive crude phytosterol. According to the method, Phytosterols are reacted to form corresponding i-steroid through tosylation and methanolysis. i-steroid on reductive ozonolysis to C-22 alcohol and conversion...

The present invention relates to a blended purified enzyme having origin of a mNPGA (mutant penicillin G acylase) from Achromobacter and Penicillin G acylase expressed in Escherichia coli. The present invention further relates to an immobilized blended purified enzyme and an enzymatic process for synthesis of semisy...

The invention discloses an improved process for production of vitamin D3 from 7- dehydrocholesterol (7- DHC) and to a simple process for recovery unreacted 7-DHC for further reuse. The invention further describes a process for isolation and purification of Vitamin D3.

The present invention discloses an improved photochemical synthesis of vitamin D3 from 7-dehydrocholesterol alone or in combination with sterol precursors in presence of the photosensitizer of Formula I in high yield and with reduced levels of impurities.

The present invention discloses an improved process for extracting cholesterol in high yield and purity from fish oil waste residue. The so obtained cholesterol of pharmaceutical grade is useful as a precursor for the preparation of vitamin D3.

The present invention discloses solvent free process for extracting cholesterol free of impurities from milk fat. The so isolated cholesterol is useful for the further preparation of vitamin D3. The present invention further provides pharmaceutical grade cholesterol from milk fat of high purity. ...

The present invention discloses an efficient process for synthesis of photosensitizer, 5-(3-pyridyl)-2,2"-bithiophene in high yield and purity.

Disclosed herein is a novel simple, short process for synthesis of the photosensitizer, 5-(3-pyridyl)-2,2"-bithiophene.

The present invention discloses novel method for synthesizing vegan cholesterol and vitamin D3 from inexpensive crude phytosterol.

Disclosed herein is an improved and efficient process for synthesis of vitamin D3 and its analogue Calcifediol from Ergosterol. Particularly, the present invention discloses the synthesis of key intermediate 3ß-tert-Butyldimethylsilyloxy-22-hydroxy-23,24-bisnorchola-5,7-diene (5), and novel intermediate ß-tert-Butyl...

The present invention discloses mutant semi-synthetic ß-lactam antibiotics synthesizing enzymes of penicillin G acylase from Achromobacter sp. CCM4824 generated by site directed mutations with substitution of one or more amino acid positions. The gene sequence encoding mutant penicillin acylase borne on plasmid pKAR...

The present invention provides a scalable two-step synthesis of molnupiravir. The reaction sequence involves the use of commercially easily available cytidine (1), and molnupiravir is formed through direct hydroxy amination of the cytidine followed by esterification of the primary alcohol without protecting dihydrox...

The present invention discloses a short, cost effective process for preparation of cholesterol from bisnoralcohol (BA).

The present invention discloses styrene butadiene rubber (SBR) composition containing the by-products based on renewable source such as calcium salt of lanolin fatty acid (CA), magnesium salt of lanolin fatty acids, pretreated calcium salt of lanolin fatty, lanolin fatty acids like crude (CLFA) and bleached lanolin ...

Disclosed herein is the enzymatic process for synthesis of Molnupiravir intermediate, 5’-isobutylester of cytidine acetonide. The process employs cytidine acetonide as starting material to prepare 5’-isobutylester of cytidine acetonide. The esterification of cytidine acetonide is carried out using Enzyme CAL B expre...

The present invention discloses an economical and improved, short process for synthesis of Molnupiravir from cytidine. The process of the present invention provides a novel intermediate N-Hydroxy Cytidine isobutyryl ester (13) which can be enzymatically converted to Molnupiravir in good yield and purity. ...

The present invention discloses an improved enzymatic synthesis of Sitalgiptin or its pharmaceutically acceptable salts in good yield and purity. The present invention provides novel intermediate (R)- 3-Acetamido-4-(2,4,5-Trifluorophenyl) butanoic acid (5).

The present invention discloses a process for conversion of bisnoralcohol into 7-DHC (provitamin of vitamin D3) and 25-OH 7-DHC (provitamin of 25-OH vitamin D3(Calcifediol), in good yields and purity.

The present invention discloses 25 Hydroxy Vitamin D3 1.25% spray dried feed composition for animals and to the process for preparation thereof.

The present invention relates to stable compositions of Cholecalciferol (Vitamin D3) and process of preparation thereof. The stable compositions of the present invention are obtained by spray drying of Vitamin D3 emulsion.

Disclosed herein is an improved, cost-effective scalable process for conversion of bisnoralcohol into 25-OH 7-DHC (provitamin of 25-OH vitamin D3(Calcifediol).

The present invention relates to lipase enzymes with enhanced activity titer by expressing a recombinant lipase gene at two different loci in a yeast host. The cultured lipase enzymes result in 2-4 fold expression of recombinant lipase activity per litre of culture media.

Disclosed herein is an industrially viable process for preparation of 7-dehycholesterol (7-DHC) from bisnoralcohol.

The present invention discloses plant based vitamin D3 (Cholecalciferol) that meets the pharmacopeia standard as well as “Green dot” specification. The present invention further discloses a commercial process for preparation of plant based vitamin D3 from plant sourced bisnoralcohol with improved yield and purity. T...