Description:TECHNICAL FIELD OF THE INVENTION:
The present invention relates to botanical based soil conditioner with bio-stimulant activity useful for improving soil health and enhancing plant growth. More specifically, the present invention relates to botanical based soil conditioner with bio-stimulant activity useful for improving soil health and enhancing plant growth and comprising natural plant extracts such as sea weed and other plant extracts loaded on a natural carrier. The present invention also relates to process for preparation of said soil conditioner.

BACKGROUND AND PRIOR ART:

Conventional agriculture practice, which mainly relies on fertilisers and pesticides, has made a significant contribution to satisfying global food demands, but at the cost of soil degradation, increasing salt concentration, decreasing soil fertility, disturbing soil rhizosphere crop yield, and soil ecosystem. Soil’s physical constraints, such as surface crusting and hardening, subsurface hard pan and compactness, high or slow permeability and extremes of consistency, soil water-related constraints, wind and water erosion, and so on, can all be attributed to a significant reduction in their production capacity. This assumes that in order to increase crop yield, soil must be kept in such physical condition that appropriate crop development can occur. Without a doubt, if soils are correctly managed for physical health, the yield potential of many crops can be greatly boosted. We can consider using organic and inorganic soil conditioners and additives to improve agricultural land and increase productivity as a means of increasing production.

Soil conditioning is any technique that improves a soil's potential to boost crop yields or improves the performance of a soil for any function, and any product used in soil conditioning is a soil conditioner. Soil Conditioners are any material(s) containing modest levels of nutrients but are handled largely for their favourable impact on the biological, physical, or chemical composition of the soil. Soil conditioning entails the production and stabilisation of soil aggregates favourable to seed germination and seedling emergence. A competent soil stabiliser for such functions will protect aggregates from disintegration caused by raindrop impact while retaining a high-water penetration capacity. Many organic compounds, gypsum, lime, natural deposits, various water-soluble polymers and cross-linked polymers that hold water in soil, live plants, microorganisms, many industrial waste products, and others are considered soil conditioners.

A soil conditioner, also known as a soil amendment, is a material that is applied to soil to enhance plant growth and health. The type of conditioner utilised is influenced by the current soil composition, climate, and plant species. Some soils lack nutrients necessary for optimal plant growth, while others store excessive or inadequate water. A conditioner, or a combination of conditioners, alters the soil's nutrient inadequacies.

Nowadays, there is an abundance of fertilisers and a greater emphasis on chemical inputs, but soil conditioners and soil amendments are sometimes overlooked. Conditioning soil is necessary for a variety of reasons. Controlling soil degradation is a critical necessity, as is improving soil air-water relations, drainage, and aggregation; reducing soil crusting and compaction, overcoming water repellence, and so on.

As a result, establishing sustainable concepts, strategies, technical improvements, and innovations is critical to increasing fertiliser efficacy, meeting the criteria of ecologically friendly crop management practises, and meeting high production expectations. Crop resilience to diseases, soil salinity, drought, heavy metals, and higher nutritional value are all becoming increasingly desirable.

Bio-based objects (including crop left over, plants, and seaweed) applied at low doses may be a potential alternative for reducing fertiliser rates while still improving plant development and most importantly as soil conditioner. Because of their beneficial characteristics, bio stimulants have grown more popular in sustainable agriculture in recent years.

They stimulate various physiological processes that promote nutrient acquisition and utilisation by plants, improve root development (the length and number of root hairs), shoot development, yield, and nutritional quality of plants, counteract the effects of biotic and abiotic stresses, improve the activity of soil microbes, and reduce the use of fertilisers and the content of undesirable compounds (e.g., nitrates and heavy metals) in cultivated plants. The effects of bio stimulants vary depending on plant species, cultivar, physiological phases, kind of substance, dose, concentration, time, and application method (e.g., foliar, soil drench, or seed treatment), as well as environmental and soil conditions.

Soil conditioner compounds are disclosed in the following patents/patent applications.
US5125950 discloses a soil conditioner composed of a soil suitable electrolyte such as sodium acetate and an invert sugar with a degree of inversion ranging from about 55% to about 75% in a weight-to-weight ratio of 20:1 to 1:10.

US6752850 discloses a liquid soil conditioning compound containing an aqueous dispersion of calcium carbonate and sulphur. Calcium and sulphur are present in sufficient quantities in the aqueous dispersion to produce calcium sulphate in situ in the soil structure, with the atomic ratio of calcium to sulphur ranging from 0.5:1 to 2.0:1.

US5779789 discloses a pellet-shaped soil conditioner containing at least 50% silica by weight and at least 20% alumina by weight, with a porosity of at least 40% by volume. In addition, the soil conditioner comprises 2-3.3% CaO, 2.9-3.4% MgO, and 7-9% FeO by weight.

US5578121 discloses a freely fiowable soil conditioner comprised of granular perlite with an unfired clay coating on the perlite grains' externally exposed surface.

US20030041639 discloses a soil conditioner containing a soil-tolerant electrolyte and sugar, the soil conditioner being a mixture of a first aqueous solution comprising sodium hydroxide, acetic acid, and 1,2,3-propanetriol produced under constant stirring and a second aqueous solution comprising saccharose, acetic acid, and potassium hydrogen tartrate produced under constant stirring.

US20040089042 discloses a potting soil and topsoil conditioning composition containing at least about 50% by volume of organic compost; at least about 5% by volume of sandy loam; at least about 5% by volume of a diatomaceous earth component containing diatomaceous earth, Phosphate, Magnesium, Potassium, and Nitrogen; and at minimum about 1% by volume of Perlite in proportions to deliver good plant nutrition, moisture retention, moisture. The structure also comprises sulphur, iron, calcium, copper, and zinc etc.

The soil conditioner formulations disclosed in the prior art do not deliver effective nutrients and minerals required for appropriate plant growth as well as boosting plant product output. Deficiency of minerals required for plants leads to inappropriate growth and numerous diseases in plants.

A need exists in the art to provide improved soil conditioner that caters to the nutritional demands of the plants, reduces the use of chemical fertilizers, are environmentally friendly, and are safe for agriculture and humans.

Hence, the present inventors have come up with an organic soil conditioner which is beneficial to plants and improves soil fertility, water retention, and overall soil structure by utilising natural plant extracts such as seaweed and other plant extracts in small amounts loaded on a natural carrier such as bentonite soil and which is economical, environmentally friendly and simple to produce and utilize.

OBJECT OF INVENTION:
It is an object of the present invention to provide an organic soil conditioner comprising a blend of standardised enriched botanical extracts and purified phyto ingredients from different plant sources which is based on bentonite granules and is useful for improving soil health, boosting plant growth and protecting plants from nematodes and fungal infections.

It is a further object of the present invention to provide a process for preparation of said botanical based soil conditioner.

SUMMARY OF THE INVENTION:

In accordance with the above objectives, the present invention provides botanical based soil conditioner with bio-stimulant activity comprising a combination of standardised enriched botanical extract and purified phyto ingredient from different plants source which provide synergic action and effectively improve soil health and structure.

In a main aspect, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract or Enriched and Purified extract of Sargassum wightii or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids , flavonoids, flavonols and flavonol glycosides extracted from said botanical source at a concentration in a range of 0.001 - 25 %, more preferably 1.0 - 6.5 %;
Sargassum vulgare extract or Enriched and Purified extract of Sargassum vulgare or Purified Phyto ingredients or marker compounds such Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides extracted from said botanical source at a concentration in a range of 0.001 - 20%, more preferably 0.5 - 6.5 %;
Cladophora rupestris extract or Enriched and Purified extract of Cladophora rupestris or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides extracted from said botanical source at a concentration in a range of 0.001- 25 %, more preferably 0.5 - 6.5 %;
Ulva lactuca extract or enriched and purified extract of Ulva lactuca or Purified Phyto ingredients or marker compounds such as Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.01- 4.5 %;
Ascophyllum nodosum extract or Enriched and Purified extract of Ascophyllum nodosum or Purified Phyto ingredients or marker compounds such as alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source at a concentration in a range between 0.01- 25 %; more preferably 0.5- 4.5 %;
Caulerpa scalpelliformis extract or Enriched and Purified extract of Caulerpa scalpelliformis or Purified Phyto ingredients or marker compounds such as tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source at a concentration in a range of 0.001 - 20 %, more preferably 0.5 - 6.5 %;
Kappaphycus alvarezii extract or Enriched and Purified extract of Kappaphycus alvarezii or Purified Phyto ingredients or marker compounds such as ?-carrageenan, IAA, Cytokinins-Tz, GA3, tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 2.5 - 8.5 %;
Macrocystis pyrifera extract or Enriched and Purified extract of Macrocystis pyrifera or Purified Phyto ingredients or marker compounds such as Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001- 20 % more preferably 0.5 - 6.5 %;
Acanthophora spicifera extract or Enriched and Purified extract of Acanthophora spicifera or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.5 - 6.5 %;
Ecklonia maxima extract or Enriched and Purified extract of Ecklonia maxima or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.5 - 6.5 %;
Saccharina japonica extract or Enriched and Purified extract of Saccharina japonica or Purified Phyto ingredients or marker compounds such as Organic matter, GA like compounds, Alginic acid and steroids extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 0.5 - 6.5 %;
Arthrospira platensis extract or Enriched and Purified extract of Arthrospira platensis or Purified Phyto ingredients or marker compounds such as Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 0.5 - 6.5 %;
Chlorella vulgaris extract or Enriched and Purified extract of Chlorella vulgaris or Purified Phyto ingredients or marker compounds such as Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.5 - 6.5 %;
Thuja occidentalis extract or Enriched and Purified extract of Thuja occidentalis or Purified Phyto ingredients or marker compounds such as essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 2.5 - 8.5 %;
Spinacia oleracea extract or Enriched and Purified extract of Spinacia oleracea or Purified Phyto ingredients or marker compounds such as phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
Tribulus terrestis extract or Enriched and Purified extract of Tribulus terrestis or Purified Phyto ingredients or marker compounds such as flavonoids, flavonol glycosides, steroidal saponins, and alkaloids extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 0.5 - 6.5 %;
Citrus aurantium extract or Enriched and Purified extract of Citrus aurantium or Purified Phyto ingredients or marker compounds such as organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 1 - 6.5 %;
Embelia ribes extract or Enriched and Purified extract of Embelia ribes or Purified Phyto ingredients or marker compounds such as acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils extracted from said botanical source at a concentration in a range of 0.001- 25 %, more preferably 1 - 6.5 %;
Ficus benghalensis extract or Enriched and Purified extract of Ficus benghalensis or Purified Phyto ingredients or marker compounds such as acidic compounds lavonoids, phenols, terpenoids, and terpenes extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 0.5 - 6.5 %;
Rosmarinus officinalis extract or Enriched and Purified extract of Rosmarinus officinalis or Purified Phyto ingredients or marker compounds such Rosmarinic acid extracted from said botanical source at a concentration in a range of 0.001-35%, more preferably 2.5 - 8.5 %;
Moringa oleifera extract or Enriched and Purified extract of Moringa oleifera or Purified Phyto ingredients or marker compounds such as Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and quercetin 3-O-glucoside extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %;
Eclipta alba extract or Enriched and Purified extract of Eclipta alba or Purified Phyto ingredients or marker compounds such as ursolic acid and oleanolic acid extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
Zingiber officinale extract or Enriched and Purified extract of Zingiber officinale or Purified Phyto ingredients or marker compounds such as Oxalic acid and Tartaric acid extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 8.5 %;
Glycyrrhiza gabra extract or Enriched and Purified extract of Glycyrrhiza gabra or Purified Phyto ingredients or marker compounds such as glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %¬;
Typha angustifolia extract or Enriched and Purified extract of Typha angustifolia or Purified Phyto ingredients or marker compounds such as sterols, terpenoids, flavones, and long-chain hydrocarbons extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 0.5 - 6.5 %;
Euphorbia hirta extract or Enriched and Purified extract of Euphorbia hirta or Purified Phyto ingredients or marker compounds such as alkaloids, phenols, flavonoids, triterpenoids and tannins extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %; and
carrier material at a concentration in a range of 60- 95 %; more preferably 75-95 %;
In an aspect, the carrier material is selected from the group comprising bentonite powder and bentonite roasted granules and the like.

In yet another aspect, the present invention provides a synergistic botanical based bio-stimulant, soil conditioner, comprising:
Ascophyllum nodosum extract in the range of 0.01 to 25 %;
Sargassum vulgare extract in the range of 0.01 to 20 %;
Citrus aurantium extract in the range of 0.001 to 30 %;
Embelia ribes in the range of 0.001 to 25 %;
Thuja occidentalis extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extracts in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Macrocystis pyrifera extract in the range of 0.01 to 20 %;
Spinacia oleracea extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Glycyrrhiza gabra extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extracts in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Cladophora rupestris extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Kappaphycus alvarezii extract in the range of 0.01 to 20 %;
Eclipta alba extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Typha angustifolia extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Saccharina japonica extract in the range of 0.01 to 20 %;
Chlorella vulgaris extract in the range of 0.01 to 20 %;
Tribulus terrestis extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Zingiber officinale extract in the range of 0.001 to 35 %;
Ficus benghalensis extracts in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Caulerpa scalpelliformis extract in the range of 0.01 to 20 %;
Acanthophora spicifera extract in the range of 0.01 to 20 %;
Spinacia oleracea extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Euphorbia hirta extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Ecklonia maxima extract in the range of 0.01 to 20 %;
Arthrospira platensis extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Glycyrrhiza gabra extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Ascophyllum nodosum extract in the range of 0.01 to 25 %;
Sargassum vulgare extract in the range of 0.01 to 20 %;
Citrus aurantium extract in the range of 0.001 to 30 %;
Embelia ribes extract in the range of 0.001 to 25 %;
Moringa oleifera extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Ascophyllum nodosum extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Citrus aurantium extract in the range of 0.001 to 30 %;
Embelia ribes extract in the range of 0.001 to 25 %;
Moringa oleifera extract in the range of 0.001 to 35 %;
Thuja occidentalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another aspect, the present invention provides a process for preparation of the botanical based soil conditioner with bio-stimulant activity, comprising:
Extracting 0.001- 25 % of Sargassum wightii extract or Enriched and Purified extract of Sargassum wightii or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids , flavonoids, flavonols and flavonol glycosides extracted from said botanical source; 0.001- 20 % of Sargassum vulgare extract or Enriched and Purified extract of Sargassum vulgare or Purified Phyto ingredients or marker compounds such Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides extracted from said botanical source; 0.001- 25 % of Cladophora rupestris extract or Enriched and Purified extract of Cladophora rupestris or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides extracted from said botanical source; 0.001- 20 % of Ulva lactuca extract or enriched and purified extract of Ulva lactuca or Purified Phyto ingredients or marker compounds such as Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source; 0.01- 25 % of Ascophyllum nodosum extract or Enriched and Purified extract of Ascophyllum nodosum or Purified Phyto ingredients or marker compounds such as alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source; 0.001- 20% of Caulerpa scalpelliformis extract or Enriched and Purified extract of Caulerpa scalpelliformis or Purified Phyto ingredients or marker compounds such as tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source; 0.001- 20 % of Kappaphycus alvarezii extract or Enriched and Purified extract of Kappaphycus alvarezii or Purified Phyto ingredients or marker compounds such as ?-carrageenan, IAA, Cytokinins-Tz, GA3,tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source; 0.001- 20 % of Macrocystis pyrifera extract or Enriched and Purified extract of Macrocystis pyrifera or Purified Phyto ingredients or marker compounds such as Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 20 % of Acanthophora spicifera extract or Enriched and Purified extract of Acanthophora spicifera or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 20 % of Ecklonia maxima extract or Enriched and Purified extract of Ecklonia maxima or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 30 % of Saccharina japonica extract or Enriched and Purified extract of Saccharina japonica or Purified Phyto ingredients or marker compounds such as Organic matter, GA like compounds, Alginic acid and steroids extracted from said botanical source; 0.001- 30% of Arthrospira platensis extract or Enriched and Purified extract of Arthrospira platensis or Purified Phyto ingredients or marker compounds such as Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid extracted from said botanical source; 0.001- 20% of Chlorella vulgaris extract or Enriched and Purified extract of Chlorella vulgaris or Purified Phyto ingredients or marker compounds such as Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl extracted from said botanical source; 0.001- 35% of Thuja occidentalis extract or Enriched and Purified extract of Thuja occidentalis or Purified Phyto ingredients or marker compounds such as essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene extracted from said botanical source; 0.001- 30% of Spinacia oleracea extract or Enriched and Purified extract of Spinacia oleracea or Purified Phyto ingredients or marker compounds such as phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids extracted from said botanical source; 0.001- 30% of Tribulus terrestis extract or Enriched and Purified extract of Tribulus terrestis or Purified Phyto ingredients or marker compounds such as flavonoids, flavonol glycosides, steroidal saponins, and alkaloids extracted from said botanical source; 0.001- 30% of Citrus aurantium extract or Enriched and Purified extract of Citrus aurantium or Purified Phyto ingredients or marker compounds such as organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins extracted from said botanical source; 0.001- 25 % of Embelia ribes extract or Enriched and Purified extract of Embelia ribes or Purified Phyto ingredients or marker compounds such as acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils extracted from said botanical source; 0.001- 35 % of Ficus benghalensis extract or Enriched and Purified extract of Ficus benghalensis or Purified Phyto ingredients or marker compounds such as acidic compounds lavonoids, phenols, terpenoids, and terpenes extracted from said botanical source; 0.001- 35 % of Rosmarinus officinalis extract or Enriched and Purified extract of Rosmarinus officinalis or Purified Phyto ingredients or marker compounds such Rosmarinic acid extracted from said botanical source; 0.001- 35 % of Moringa oleifera extract or Enriched and Purified extract of Moringa oleifera or Purified Phyto ingredients or marker compounds such as Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and quercetin 3-O-glucoside extracted from said botanical source; 0.001- 30% of Eclipta alba extract or Enriched and Purified extract of Eclipta alba or Purified Phyto ingredients or marker compounds such as ursolic acid and oleanolic acid extracted from said botanical source; 0.001- 35 % of Zingiber officinale extract or Enriched and Purified extract of Zingiber officinale or Purified Phyto ingredients or marker compounds such as Oxalic acid and Tartaric acid extracted from said botanical source; 0.001- 35 % of Glycyrrhiza gabra extract or Enriched and Purified extract of Glycyrrhiza gabra or Purified Phyto ingredients or marker compounds such as glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin extracted from said botanical source; 0.001- 35 % of Typha angustifolia extract or Enriched and Purified extract of Typha angustifolia or Purified Phyto ingredients or marker compounds such as sterols, terpenoids, flavones, and long-chain hydrocarbons extracted from said botanical source; 0.001- 35 % of Euphorbia hirta extract or Enriched and Purified extract of Euphorbia hirta or Purified Phyto ingredients or marker compounds such as alkaloids, phenols, flavonoids, triterpenoids and tannins extracted from said botanical source;
blending the above extracts at specific temperature ranging from 30oC to 45oC and stirring at specific RPM ranging from 50 -800 RPM;
stirring the mixture obtained in step (b) at 600 to 1000 rpm RPM at 35-45°C in closed mixing vessel for continued stirring followed by homogenizing the mix with the homogenizer fitted towards the bottom of vessel and with the speed of 3700~27000 rpm to reduce the particle size.;
passing the homogenized mixture obtained in step (c) through in line shear pump with 1800 RPM to 5800 RPM to reduce particle size to obtain the nano emulsion with particle size ranging from 10 to 1000 nano meter;
passing the mixture obtained in step (d) through High-pressure homogenization to reduce the particle size below 100 nano meters;
passing the mixture obtained in step (g) through sparkler filter having seven layers of membrane filters with pore size less than 1-2 micron to achieve desired filtration;
loading the product obtained in step (f) on carrier material by using high speed jet sprayer in presence of dry air at temp 40 degree celsius for easy drying; and
recovering the final product.

The soil conditioner the present invention is useful for providing critical nutrients, minerals and trace elements to plants, boosting plant germination, improving plant yield, increasing plant resistance to pests and diseases and protecting plants from nematodes and fungal infections.

Description of Figures:
Figure 1: Bio stimulant effect of Botanical based soil conditioner in Rice
Figure 2: Percentage decrease over control in nematode population after application of Botanical based soil conditioner in Rice
Figure 3: Percentage decrease over control in no. of galls/root system and Percentage decrease in no. egg masses/root system after application of Botanical based soil conditioner in Rice
Figure 4: Percentage decrease in nematode population after application of Botanical based soil conditioner in Tomato
Figure 5: Percentage decrease in no. of galls/root system and Percentage decrease in no. egg masses/root system after application of Botanical based soil conditioner in Tomato
Figure 6: Effect of botanical based soil conditioner granules on fruit yield in banana
Figure 7: Effect of botanical based soil conditioner granules on grain yield of paddy
Figure 8: Effect of botanical based soil conditioner granules on cane yield in sugarcane
Figure 9: Effect of botanical based soil conditioner granules on fruit yield in tomato

DETAILED DESCRIPTION OF THE INVENTION:

The invention will now be described in detail in connection with certain preferred and
optional embodiments, so that various aspects thereof may be more fully understood and
appreciated.

Seaweed extracts show presence of high concentration of bioactive chemicals, and thus seaweed extracts have been utilised for decades as soil conditioners or plant stimulators to boost the productivity of agronomic and horticultural crops. Some treatments are direct, while others are aimed at increasing nutrient availability in the soil. This is because plants only take nutrients through their leaf surfaces and roots. Seaweeds also include a variety of energy minerals and chemical compounds, such as complex polysaccharides including laminarin, fucoidan, alginates, and plant hormones that promote plant growth.

Seaweed extracts have been shown to increase crop yields, uptake of inorganic soil nutrients and resistance to pests and environmental stress conditions. Seaweed extracts also reduce fruit losses during storage and increase their reproductive capacity.

Bentonite is a naturally occurring soil amendment that is abundant in India. Because of its excellent adsorption capabilities, bentonite is now widely utilised for adsorbing metal ions and dye, disposing of radioactive wastes, and cleaning viral RNA and waste water. Furthermore, its usage as a soil amendment could be a viable solution to some of the agronomic/environmental issues associated with drought stress and soil degradation in agricultural systems. Bentonite has been widely investigated and proven to boost soil moisture and water storage, increase millet production, and improve water usage efficiency other research has found that adding clay as a soil supplement to sandy soil can improve soil accessible water, organic carbon, and potassium, as well as increase crop emergence and yield. This was related to increased soil aggregation, which improves soil porosity, soil water-holding capacity, soil fertility, and crop growth.

In an embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract or Enriched and Purified extract of Sargassum wightii or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids, flavonoids, flavonols and flavonol glycosides extracted from said botanical source at a concentration in a range of 0.001- 25 %, more preferably 1.0 - 6.5 %;
Sargassum vulgare extract or Enriched and Purified extract of Sargassum vulgare or Purified Phyto ingredients or marker compounds such Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides extracted from said botanical source at a concentration in a range of 0.001-20%, more preferably 0.5 - 6.5;
Cladophora rupestris extract or Enriched and Purified extract of Cladophora rupestris or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides extracted from said botanical source at a concentration in a range of 0.001-25 %, more preferably 0.5 - 6.5 %;
Ulva lactuca extract or enriched and purified extract of Ulva lactuca or Purified Phyto ingredients or marker compounds such as Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.01- 4.5 %;
Ascophyllum nodosum extract or Enriched and Purified extract of Ascophyllum nodosum or Purified Phyto ingredients or marker compounds such as alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source at a concentration in a range between 0.01- 25 %; more preferably 0.5- 4.5 %;
Caulerpa scalpelliformis extract or Enriched and Purified extract of Caulerpa scalpelliformis or Purified Phyto ingredients or marker compounds such as tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.5 - 6.5 %;
Kappaphycus alvarezii extract or Enriched and Purified extract of Kappaphycus alvarezii or Purified Phyto ingredients or marker compounds such as ?-carrageenan, IAA, Cytokinins-Tz, GA3, tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 2.5 - 8.5 %;
Macrocystis pyrifera extract or Enriched and Purified extract of Macrocystis pyrifera or Purified Phyto ingredients or marker compounds such as Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001- 20 % more preferably 0.5 - 6.5 %;
Acanthophora spicifera extract or Enriched and Purified extract of Acanthophora spicifera or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.5 - 6.5 %;
Ecklonia maxima extract or Enriched and Purified extract of Ecklonia maxima or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.5 - 6.5 %;
Saccharina japonica extract or Enriched and Purified extract of Saccharina japonica or Purified Phyto ingredients or marker compounds such as Organic matter, GA like compounds, Alginic acid and steroids extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 0.5 - 6.5 %;
Arthrospira platensis extract or Enriched and Purified extract of Arthrospira platensis or Purified Phyto ingredients or marker compounds such as Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 0.5 - 6.5 %;
Chlorella vulgaris extract or Enriched and Purified extract of Chlorella vulgaris or Purified Phyto ingredients or marker compounds such as Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl extracted from said botanical source at a concentration in a range of 0.001- 20 %, more preferably 0.5 - 6.5 %;
Thuja occidentalis extract or Enriched and Purified extract of Thuja occidentalis or Purified Phyto ingredients or marker compounds such as essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 2.5 - 8.5 %;
Spinacia oleracea extract or Enriched and Purified extract of Spinacia oleracea or Purified Phyto ingredients or marker compounds such as phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
Tribulus terrestis extract or Enriched and Purified extract of Tribulus terrestis or Purified Phyto ingredients or marker compounds such as flavonoids, flavonol glycosides, steroidal saponins, and alkaloids extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 0.5 - 6.5 %;
Citrus aurantium extract or Enriched and Purified extract of Citrus aurantium or Purified Phyto ingredients or marker compounds such as organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins extracted from said botanical source at a concentration in a range of 0.001- 30 %, more preferably 1 - 6.5 %;
Embelia ribes extract or Enriched and Purified extract of Embelia ribes or Purified Phyto ingredients or marker compounds such as acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils extracted from said botanical source at a concentration in a range of 0.001- 25 %, more preferably 1 - 6.5 %;
Ficus benghalensis extract or Enriched and Purified extract of Ficus benghalensis or Purified Phyto ingredients or marker compounds such as acidic compounds lavonoids, phenols, terpenoids, and terpenes extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 0.5 - 6.5 %;
Rosmarinus officinalis extract or Enriched and Purified extract of Rosmarinus officinalis or Purified Phyto ingredients or marker compounds such Rosmarinic acid extracted from said botanical source at a concentration in a range of 0.001-35%, more preferably 2.5 - 8.5 %;
Moringa oleifera extract or Enriched and Purified extract of Moringa oleifera or Purified Phyto ingredients or marker compounds such as Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and quercetin 3-O-glucoside extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %;
Eclipta alba extract or Enriched and Purified extract of Eclipta alba or Purified Phyto ingredients or marker compounds such as ursolic acid and oleanolic acid extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
Zingiber officinale extract or Enriched and Purified extract of Zingiber officinale or Purified Phyto ingredients or marker compounds such as Oxalic acid and Tartaric acid extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 8.5 %;
Glycyrrhiza gabra extract or Enriched and Purified extract of Glycyrrhiza gabra or Purified Phyto ingredients or marker compounds such as glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %;
Typha angustifolia extract or Enriched and Purified extract of Typha angustifolia or Purified Phyto ingredients or marker compounds such as sterols, terpenoids, flavones, and long-chain hydrocarbons extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 0.5 - 6.5 %;
Euphorbia hirta extract or Enriched and Purified extract of Euphorbia hirta or Purified Phyto ingredients or marker compounds such as alkaloids, phenols, flavonoids, triterpenoids and tannins extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %; and
carrier material at a concentration in a range of 60-95 %; more preferably 75-95 %;
The combination of these ingredients has been found to have synergistic benefits and effects, despite the fact that each of these ingredients has unique qualities and properties which makes it as an ideal soil conditioner.

In another embodiment, the carrier material is selected from the group comprising bentonite powder, bentonite roasted granules and the like.
The present botanical based soil conditioner is composed of a mixture of natural and standardised botanical extracts, more specifically enriched active ingredient from botanical source with synergistic interactions with each other. At the same time, they have low toxicity for mammals, little environmental persistence, and are generally harmless.

In another embodiment, the botanical based soil conditioner of the present invention comprising combination of standardised enriched botanical extract and purified phyto ingredient with a synergistic biological action that provides essential nutrients, minerals and trace elements to plants and improves plant yield.

In another embodiment, the botanical based soil conditioner with bio-stimulant activity of the present invention include enriched and purified Phyto ingredients such as Organic matter, Humic acid, saponins, polyphenols, alkaloids, flavonoids, proteins terpenoids, or secondary metabolites, other plant growth promoting substances from botanicals which produce important synergic interactions in their biological activity. The combination of these ingredients has been found to have synergistic benefits and effects, despite the fact that each of these ingredients has unique qualities and properties.

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Ascophyllum nodosum extract in the range of 0.01 to 25 %;
Sargassum vulgare extract in the range of 0.01 to 20 %;
Citrus aurantium extract in the range of 0.001 to 30 %;
Embelia ribes in the range of 0.001 to 25 %;
Thuja occidentalis extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extracts in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Macrocystis pyrifera extract in the range of 0.01 to 20 %;
Spinacia oleracea extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Glycyrrhiza gabra extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extracts in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Cladophora rupestris extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Kappaphycus alvarezii extract in the range of 0.01 to 20 %;
Eclipta alba extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Typha angustifolia extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Saccharina japonica extract in the range of 0.01 to 20 %;
Chlorella vulgaris extract in the range of 0.01 to 20 %;
Tribulus terrestis extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Zingiber officinale extract in the range of 0.001 to 35 %;
Ficus benghalensis extracts in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Caulerpa scalpelliformis extract in the range of 0.01 to 20 %;
Acanthophora spicifera extract in the range of 0.01 to 20 %;
Spinacia oleracea extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Euphorbia hirta extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Sargassum wightii extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Ecklonia maxima extract in the range of 0.01 to 20 %;
Arthrospira platensis extract in the range of 0.001 to 30 %;
Moringa oleifera extract in the range of 0.001 to 25 %;
Glycyrrhiza gabra extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Ascophyllum nodosum extract in the range of 0.01 to 25 %;
Sargassum vulgare extract in the range of 0.01 to 20 %;
Citrus aurantium extract in the range of 0.001 to 30 %;
Embelia ribes extract in the range of 0.001 to 25 %;
Moringa oleifera extract in the range of 0.001 to 35 %;
Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

In yet another embodiment, the present invention provides a botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
Ascophyllum nodosum extract in the range of 0.01 to 25 %;
Ulva lactuca extract in the range of 0.01 to 20 %;
Citrus aurantium extract in the range of 0.001 to 30 %;
Embelia ribes extract in the range of 0.001 to 25 %;
Moringa oleifera extract in the range of 0.001 to 35 %;
Thuja occidentalis extract in the range of 0.001 to 35 %; and
Bentonite Granules in the range of 60 to 95 %

The synergistic effects of each botanical ingredient were tested in combination with other botanical ingredients at a ratio of 1:1 and compared to their respective individual effects on soil physical structure and on plants growth in polyhouse. A combination is said to be synergistic if it boost the plant growth in comparison with control and individual along improving soil structure which include water retention, pH, EC and other chemical analysis.
In another embodiment, botanical based soil conditioner of the present invention is prepared by using various standardised enriched botanical extract and purified phyto ingredient from different plants source which provide synergistic effect and effectively improve soil health and structure.

In yet another embodiment, the present invention provides a process for preparation of the botanical based soil conditioner with bio-stimulant activity comprising:
Extracting 0.001- 25 % of Sargassum wightii extract or Enriched and Purified extract of Sargassum wightii or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids , flavonoids, flavonols and flavonol glycosides extracted from said botanical source; 0.001- 20 % of Sargassum vulgare extract or Enriched and Purified extract of Sargassum vulgare or Purified Phyto ingredients or marker compounds such Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides extracted from said botanical source; 0.001- 25 % of Cladophora rupestris extract or Enriched and Purified extract of Cladophora rupestris or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides extracted from said botanical source; 0.001- 20 % of Ulva lactuca extract or enriched and purified extract of Ulva lactuca or Purified Phyto ingredients or marker compounds such as Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source; 0.001- 25 % of Ascophyllum nodosum extract or Enriched and Purified extract of Ascophyllum nodosum or Purified Phyto ingredients or marker compounds such as alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source; 0.001- 20 % of Caulerpa scalpelliformis extract or Enriched and Purified extract of Caulerpa scalpelliformis or Purified Phyto ingredients or marker compounds such as tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source; 0.001- 20 % of Kappaphycus alvarezii extract or Enriched and Purified extract of Kappaphycus alvarezii or Purified Phyto ingredients or marker compounds such as ?-carrageenan, IAA, Cytokinins-Tz, GA3,tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source; 0.001- 20 % of Macrocystis pyrifera extract or Enriched and Purified extract of Macrocystis pyrifera or Purified Phyto ingredients or marker compounds such as Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 20 % of Acanthophora spicifera extract or Enriched and Purified extract of Acanthophora spicifera or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 20 % of Ecklonia maxima extract or Enriched and Purified extract of Ecklonia maxima or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 30 % of Saccharina japonica extract or Enriched and Purified extract of Saccharina japonica or Purified Phyto ingredients or marker compounds such as Organic matter, GA like compounds, Alginic acid and steroids extracted from said botanical source; 0.001- 30 % of Arthrospira platensis extract or Enriched and Purified extract of Arthrospira platensis or Purified Phyto ingredients or marker compounds such as Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid extracted from said botanical source; 0.001- 20 % of Chlorella vulgaris extract or Enriched and Purified extract of Chlorella vulgaris or Purified Phyto ingredients or marker compounds such as Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl extracted from said botanical source; 0.001-35 % of Thuja occidentalis extract or Enriched and Purified extract of Thuja occidentalis or Purified Phyto ingredients or marker compounds such as essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene extracted from said botanical source; 0.001- 30 % of Spinacia oleracea extract or Enriched and Purified extract of Spinacia oleracea or Purified Phyto ingredients or marker compounds such as phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids extracted from said botanical source; 0.001- 30 % of Tribulus terrestis extract or Enriched and Purified extract of Tribulus terrestis or Purified Phyto ingredients or marker compounds such as flavonoids, flavonol glycosides, steroidal saponins, and alkaloids extracted from said botanical source; 0.001- 30 % of Citrus aurantium extract or Enriched and Purified extract of Citrus aurantium or Purified Phyto ingredients or marker compounds such as organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins extracted from said botanical source; 0.001- 25 % of Embelia ribes extract or Enriched and Purified extract of Embelia ribes or Purified Phyto ingredients or marker compounds such as acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils extracted from said botanical source; 0.001-35 % of Ficus benghalensis extract or Enriched and Purified extract of Ficus benghalensis or Purified Phyto ingredients or marker compounds such as acidic compounds lavonoids, phenols, terpenoids, and terpenes extracted from said botanical source; 0.001-35 % of Rosmarinus officinalis extract or Enriched and Purified extract of Rosmarinus officinalis or Purified Phyto ingredients or marker compounds such Rosmarinic acid extracted from said botanical source; 0.001-35 % of Moringa oleifera extract or Enriched and Purified extract of Moringa oleifera or Purified Phyto ingredients or marker compounds such as Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and quercetin 3-O-glucoside extracted from said botanical source; 0.001- 30 % of Eclipta alba extract or Enriched and Purified extract of Eclipta alba or Purified Phyto ingredients or marker compounds such as ursolic acid and oleanolic acid extracted from said botanical source; 0.001-35 % of Zingiber officinale extract or Enriched and Purified extract of Zingiber officinale or Purified Phyto ingredients or marker compounds such as Oxalic acid and Tartaric acid extracted from said botanical source; 0.001-35 % of Glycyrrhiza gabra extract or Enriched and Purified extract of Glycyrrhiza gabra or Purified Phyto ingredients or marker compounds such as glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin extracted from said botanical source; 0.001-35 % of Typha angustifolia extract or Enriched and Purified extract of Typha angustifolia or Purified Phyto ingredients or marker compounds such as sterols, terpenoids, flavones, and long-chain hydrocarbons extracted from said botanical source; 0.001-35 % of Euphorbia hirta extract or Enriched and Purified extract of Euphorbia hirta or Purified Phyto ingredients or marker compounds such as alkaloids, phenols, flavonoids, triterpenoids and tannins extracted from said botanical source;
blending the above extracts at specific temperature ranging from 30oC to 45oC and stirring at specific RPM ranging from 50 -800 RPM;
stirring the mixture obtained in step (b) at 600 to 1000 rpm RPM at 35-45°C in closed mixing vessel for continued stirring followed by homogenizing the mix with the homogenizer fitted towards the bottom of vessel and with the speed of 3700~27000 rpm to reduce the particle size.;
passing the homogenized mixture obtained in step (c) through in line shear pump with 1800 RPM to 5800 RPM to reduce particle size to obtain the nano emulsion with particle size ranging from 10 to 1000 nano meter;
passing the mixture obtained in step (d) through High-pressure homogenization to reduce the particle size below 100 nano meters;
passing the mixture obtained in step (g) through sparkler filter having seven layers of membrane filters with pore size less than 1-2 micron to achieve desired filtration;
loading the product obtained in step (f) on carrier material by using high speed jet sprayer in presence of dry air at temp 40 degree celsius for easy drying; and
recovering the final product.

In an embodiment, the mixing vessel in step (c) is made up of stainless steel 316 grade with outer jacket for maintaining temperature with a stirrer such as blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer, universal stirrer and the like.
In another embodiment, the homogenization of step (c) is done under very high pressures, sheer, turbulence, acceleration and impact, to make the particles more stable and effective.
In another embodiment, the high-pressure homogenization of step (e) is done at speed of 4000 rpm to 10000 rpm and pressure max up to 4,200 bar (60,000 psi).
In another embodiment, the process for producing the aforementioned standardised and enriched extract and purified Phyto ingredients, active ingredient, or marker compound is obtained by performing extraction using a variety of processes, such as aqueous and solvent, extraction, where the solvent used is are not limited to water, ethanol, methanol, acetone, further purification of Phyto ingredient is carried by using liquid liquid extraction and various chromatograph techniques to obtain highly pure form of Phyto ingredient or marker compound.

In another embodiment, the solvent used in the solvent extraction includes but not limited to alcohols, hydrocarbons, ketones, esters, ethers, and the like alone or in combination thereof.

In yet another embodiment, further purification of Phyto ingredient is carried out by using liquid liquid extraction, various chromatograph techniques like liquid-liquid chromatography, column chromatography, affinity chromatography, silica gel chromatography, ion exchange chromatography and other purification like precipitation and crystallisation to obtain highly pure form of Phyto ingredient or marker compound.

Instead of using individual standardized extract or purified Phyto ingredients, which can be used separately or together, a holistic approach using extract and Phyto ingredients was used which is more beneficial to soil and plants. The botanical extract used as ingredients in formulation are standardized and quantified as well as Phyto ingredient profile has been explored.

In an embodiment, the standardize botanical extract are blend with each other and this blend is prepared at specific temperature ranging from 30°C to 45°C and stirring at specific RPM ranging from 50 -800 RPM, particle size of active blend has been reduce to achieve nano range by using various modules like Filter press, shear pump, homogenizer and High pressure homogenization (pressure max up to 45,000 PSI) obtain Nano formulation with average particle size range between 10 -1000 nm.

In another embodiment, the active blend form is loaded on carrier material selected from the group comprising bentonite powder or granules and the like by using high speed jet sprayer.

In another embodiment, the extraction techniques include but not limited to hydrophilic extraction, super critical carbon dioxide extraction, steam distillation, solvent extraction and Soxhlet extraction.

In another embodiment, the solvent used in the solvent extraction includes but not limited to alcohols, hydrocarbons, ketones, esters, ethers, water and the like alone or in acombination thereof, more preferably water, ethanol, methanol, ethyl acetate, hexane, acetone.

In another embodiment, the enrichment and purification of the extract using various techniques include but not limited to liquid-liquid extraction base on polarity of phyto ingredient, column chromatography more preferably Liquid- liquid chromatography by using various medium and matrix, precipitation and crystallisation etc. to obtain purified phyto ingredients such as but not limited to IBA, IAA, GA3, Endogenous Salicylic acid, ulvan, carotenoid tannins, flavanoids, glycosides, phenols, saponins, terpenoids sterols, terpenoids, flavonoids, glycosides, alkaloids and carbohydrates alginates and fucoidan, laminarins, Betaines, glycinebetaine, polyphenols eckol, phloroglucinol, polyamines, GA, castasterone, brassinolide, Polysaccharides Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin Kaempferol 3-O-glucoside, isorhamnetin 3-O-galactoside, rutin, phenylalanine and tryptophan ursolic acid, oleanolic acid Oxalic acid, Tartaric acid glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin rosmaric acid, ascorbic acid, quercetin 3-O-glucoside and the like.

In another embodiment, the botanical based soil conditioner of the present invention may be in the form of liquid, paste, powder or powder converted into granule or tablet form and the like, more preferably in liquid form.
Since most organic soil conditioner lack standardisation, the formulation that is the result is standardised for a few chosen phytochemicals to ensure quality.
In another embodiment, the botanical based soil conditioner of the present invention is further validated by conducting bio efficacy tests in vitro and in vivo on a more precise on crops.
It performs as expected in both in vitro and in vivo studies, competing with top commercial soil conditioners that are primarily harmful to the environment and harm unintended organisms, causing rhizosphere disruption.
In another embodiment, the botanical based soil conditioner of the present invention is evaluated to show bio stimulant activity in plants. Significant plant growth stimulant activity is observed with respect to height, growth, chlorophyll content, increase in flower and fruit number and subsequently the yield.
In another embodiment, the present botanical based soil conditioner discloses dose dependent bio-stimulant activity as demonstrated in the Tables 12, 16, 21, 22, 25, 26, 29, 30, 33 and 34.
In another embodiment, the botanical based soil conditioner of the present invention significantly improved soil physico-chemical properties such as soil pH, electrical conductivity, soil porosity, organic carbon and bulk density as demonstrated in Tables 20, 24, 28 and 32.
In another embodiment, the botanical based soil conditioner of the present invention exhibits biostimulant activity and thereby improves plant growth and crop yield.

In another embodiment, the botanical based soil conditioner of the present invention relieves plant stress caused by biotic and abiotic factors. It also improves soil nutrient levels.
In yet another embodiment, the botanical based soil conditioner, formulation prepared have a holistic defence approach to control destructive microbial pest and the risk of resistance development is low as it is multi-ingredient product as well the product exhibit multi-mode action.
In another embodiment, the botanical based soil conditioner of the present invention is efficient after application to improve soil health and nourish the growth of beneficial organisms in soil, and growth stimulating activity is noticeable.
In yet another embodiment, the botanical based soil conditioner of the present invention inhibits the growth of dangerous nematodes, which are primarily responsible for root knots and other symptoms. The present botanical-based soil conditioner has nematicidal activity and protects plants from nematode infestation. Same is observed in in vitro and in vivo trial.
In another embodiment, the present botanical based soil conditioner discloses dose dependent nematicidal activity as demonstrated in the Tables 13, 14, 17 and 18.
In another embodiment, the botanical based soil conditioner of the present invention is provided at a concentration ranging between 4-8 kg per acer or 10 – 20 kg per hectar, more precise 8 kg per acer or 18 kg per hectar
In another embodiment, the botanical based soil conditioner of the present invention can be applied by sprinkler application, sprayer application or drip application. The soil conditioner may preferably be applied by sprayer application such as foliar sprays, sprays to be applied to plants shoots and the like.

In another embodiment, the botanical based soil conditioner of the present invention shows high specificity for the target pest, low toxicity for mammals and low environmental persistence. It attacks pests through contact, fumigant, and systematic action, and it is simple to use.

In another embodiment, the botanical based soil conditioner of the present invention exhibits synergism at specific concentration and thereby improves soil health, nourishes the growth of beneficial organisms in soil, and improves growth stimulating activity.

In another embodiment, the botanical based soil conditioner of the present invention is useful for boosting plant germination, improving plant yield, providing critical nutrients, minerals and trace elements to plants, increasing plant resistance to pests and diseases and protecting plants from nematodes and fungal plant pathogens.

In another embodiment, the botanical based soil conditioner of the present invention is effective against nematodes such as Root knot nematodes (meloidogyne spp.), cyst nematodes (Heterodera and Globoders spp.), Root lesion nematodes (pratylenchus spp.) and fungal plant pathogens include Armillaria mellea, Clitocybe tabescens, and Fusarium, and many oomycetes.

In yet another embodiment, the botanical based soil conditioner of the present invention is environmentally safe, biodegradable, and residue-free.
In another embodiment, the botanical based soil conditioner of the present invention are easily taken up by plants which significantly boost the plant growth with respect of height, growth, chlorophyll content, increase in flower and fruit number and subsequently the yield.

In another embodiment, the botanical based soil conditioner of the present invention is effective against Nematodes within 96 hrs of application with low risk of resistance development.

In another embodiment, the botanical based soil conditioner of the present invention is effective at improving soil physico-chemical properties, improving nutrient uptake, increasing fruit yield and overall plant growth as demonstrated in Examples given below.

In an embodiment, the present invention discloses a method for improving soil health and promoting the growth of the plants comprising applying the present soil conditioner to the plant as a basal treatment and top dressing in an effective amount.

The soil conditioner of the present invention has multiple advantages/benefits such as –
The soil conditioner of the present invention helps in improving soil health and plant growth. It also helps in increasing plant growth and enhancing yield of the crops.
The risk of resistance development in pests is low as the present soil conditioner it is multi-ingredient product as well the product exhibits multi-mode action.
It is environmentally safe, relatively inexpensive, safe for agriculture and humans.
It is biodegradable, residue-free and thus minimizes the chemical residue on our planet.
It improves soil structure by increasing water retention capacity, minimising erosion, and preventing compaction. This promotes plant growth by allowing roots to more easily penetrate the soil and acquire nutrients and water.
It improves nutrient availability by providing a slow-release supply of nutrients like nitrogen, phosphorous, and potassium. This promotes healthier and more productive plant growth while avoiding the negative environmental effects of synthetic fertilisers.
It improves soil microbiology by introducing beneficial microbes such as bacteria and fungi. These microbes can aid in the breakdown of organic matter, the improvement of nutrient cycling, and the enhancement of soil fertility.
It reduces the need for artificial inputs such as fertilisers and pesticides, which can have negative environmental consequences. This can save producers money while also reducing their environmental impact.

The following example, which includes preferred embodiments, will serve to illustrate the
practice of this invention, it being understood that the particulars shown are by way of example and for purpose of illustrative discussion of preferred embodiments of the invention.

Examples:
Example 1: List of Ingredient used in formulation of botanical based soil conditioner along with their details:
Table 1

Sr. No. Botanicals Different form of botanical extracts used in formulation of botanical based soil conditioner Part Percentage used in formulation of botanical based soil conditioner Percent Range of botanical extracts Soil conditioner / Bio-stimulant Properties
1. Sargassum wightii Sargassum wightii extract 6.0 % 0.001-35 % 1. Enhancing the soil fertility
2. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
3. Suppressive effect on root pathogens of tomato and sunflower by reducing fungal root infection and nematode's galls on roots and nematode's penetration in roots.
Enriched & Purified extract of Sargassum wightii 3.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids , flavonoids, flavonols and flavonol glycosides 1.0 % 0.001-15 %
2. Sargassum vulgare Sargassum vulgare extract 8.0 % 0.001-35 % 1. Enhancing the soil fertility
2. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
3. Increase root and shoot of plants
4. triggered early flowering and increased fruit set in crops, enhance plant defence system
Enriched & Purified extract of Sargassum vulgare 2.5 % 0.01-20 %
(Purified Phyto ingredients or marker compounds) Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides 1.5 % 0.001-15 %
3. Cladophora rupestris Cladophora rupestris extract 10.0 % 0.001-35 % 1. Enhancing the soil fertility
2. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
3. Increase water holding capacity
4. triggered early flowering and increased fruit set in crops, enhance plant defence system
Enriched & Purified extract of Cladophora rupestris 8.0 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides
4.5 % 0.001-15 %

4. Ulva lactuca Ulva lactuca extract 9.0 % 0.001-35 % 1. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
2. Increase water holding capacity
3. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients.
Enriched & Purified extract of Ulva lactuca 6.0 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides 3.5 % 0.001-15 %
5. Ascophyllum nodosum Ascophyllum nodosum extract 15.0 % 1. Soil-conditioning properties due to alginic acid, improve water retention capacity of the soil, stimulate soil microbial activity.
2. Increase Phosphorus and sulphur uptake, increased biomass, protein content, chlorophyll and carotenoid content, flavonoids and phenolics, and increased antioxidant activity.
3.Improve plant Vigor, increase root development, enhance chlorophyll synthesis, promote earlier flowering, enhance fruit set and uniformity of fruit, reduce pod shatter, delay senescence.
4. Enhance tolerance to abiotic stress.

Enriched & Purified extract of Ascophyllum nodosum 8.0 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides
6.5 % 0.001-15 %
6. Caulerpa scalpelliformis Caulerpa scalpelliformis extract 13.4 % 0.001-35 % 1. Increasing the growth promoting hormones and nutrients, increased seed germination, increase lateral roots.
2. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients.

Enriched & Purified extract of Caulerpa scalpelliformis 8.3 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) tannins, flavanoids, glycosides, phenols, saponins, terpenoids 5.5 % 0.001-15 %
7. Kappaphycus alvarezii Kappaphycus alvarezii extract 10.0 % 0.001-35 % 1. Increase Uptake of nutrients (Mg and Fe) and photosynthetic rate, promotion of overall vigor and the growth of plants.
2. Increased root length, enhanced chlorophyll content and carotenoids, and tissue water content under salinity and drought stress.
3.Accumulated Osmo protectants including proline, amino acids, and total protein

Enriched & Purified extract of Kappaphycus alvarezii 5.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) ?-carrageenan, IAA, Cytokinins-Tz, GA3,tannins, flavanoids, glycosides, phenols, saponins, terpenoids 2.5 % 0.001-15 %
8. Macrocystis pyrifera Macrocystis pyrifera extract 8.0 % 0.001-35 % 1. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
2. Significant increase in flower number, inflorescence number, flower to fruit ratio, and increased fruit number and size, enhance plant defence system and increase in defense enzyme activity.
Enriched & Purified extract of Macrocystis pyrifera 5.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds)Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids 2.0 % 0.001-15 %
9. Acanthophora spicifera Acanthophora spicifera extract 15.0 % 0.001-35 % 1. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
2. Flowers, inflorescences, flower-to-fruit ratio, fruit size, fruit number, and defence enzyme activity all significantly increase, as does the plant's defence system and activity.
Enriched & Purified extract of Acanthophora spicifera 10.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids 8.0 % 0.001-15 %
10. Ecklonia maxima Ecklonia maxima extract 12.0 % 0.001-35 % 1. Improve soil structure by boosting plant nutrients, aeration, and water retention.
2. Increase protein contain in crop, increase in root density,
3.Increases in plant growth and yield parameters as well as an
increase of protein and nutrient content.
Enriched & Purified extract of Ecklonia maxima 6.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids 3.0 % 0.001-15 %
11. Saccharina japonica Saccharina japonica extract 8.0 % 0.001-35 % 1.Increase soil aeration, increase water holding capacity, and add plant nutrients to strengthen soil structure.
2.Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
Enriched & Purified extract of Saccharina japonica 6.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Organic matter, GA like compounds, Alginic acid and steroids 2.5 % 0.001-15 %
12. Arthrospira platensis Arthrospira platensis extract 10.0 % 0.001-35 % 1. It enhanced total nitrogen and accessible phosphorus in the soil, improve soil nitrate levels
Enriched & Purified extract of Arthrospira platensis 8.0 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid 3.5 % 0.001-15 %

13.

Chlorella vulgaris Chlorella vulgaris extract 12.0 % 0.001-35 % 1.It can be used as bio-fertilizer to enhance yield, improves the biological (CO2 evolution, dehydrogenase
activity, nitrogenase activity) and chemical
properties of the soil (pH, available-N, available-P, available-K)
Enriched & Purified extract of Chlorella vulgaris 6.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl 3.0 % 0.001-15 %
14. Thuja occidentalis Thuja occidentalis aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 10.0 % 0.001-35 % 1. soil pH shifted toward neutrality, and substantial increments were observed in water holding capacity (WHC), cation exchange capacity (CEC), and N/P availability,
2.Improvements in leaf area index (LAI), leaf number, chlorophyll content, nitrate reductase (NR) activity.
Enriched & Purified extract of Thuja occidentalis 8.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene 4.0 % 0.001-15 %

15.

Spinacia oleracea Spinacia oleracea aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. Deliver all major macro- and micronutrients to soil, improving the fertility.
2. Improve water holding capacity of soil.
Enriched & Purified extract of Spinacia oleracea 9.5 % 0.001-25 %
(Purified Phyto ingredients or marker compounds) phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids. 6.0 % 0.001-15 %
16. Tribulus terrestis Tribulus terrestis aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 10.0 % 0.001-35 % 1. Significant reduction of ammonia volatilization from soil
Enriched & Purified extract of Tribulus terrestis 7.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds flavonoids, flavonol glycosides, steroidal saponins, and alkaloids. 5.0 % 0.001-15 %
17. Citrus aurantium Citrus aurantium aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 15.0 % 0.001-35 % 1. Reduce pH of soil.
2. release trap nutrient in soil and make it available for plants.
3.Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
Enriched & Purified extract of Citrus aurantium 10.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins 8.0 % 0.001-15 %

18.

Embelia ribes Embelia ribes aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 13.0 % 0.001-35 % 1. Reduce pH of soil.
2. release trap nutrient in soil and make it available for plants.
3.Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
Enriched & Purified extract of Embelia ribes 9.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils 5.0 % 0.001-15 %
19. Ficus benghalensis

Ficus benghalensis
aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 13.0 % 0.001-35 % 1. Reduce pH of soil.
2.Improve soil structure.
Enriched & Purified extract of Ficus benghalensis 9.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds acidic compounds lavonoids, phenols, terpenoids, and terpenes 5.0 % 0.001-15 %
20.

Rosmarinus officinalis

Rosmarinus officinalis
aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. Reduce pH of soil
2. Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
Enriched & Purified extract of Rosmarinus officinalis 3.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds Rosmarinic acid. 4.0 % 0.001-15 %
21. Moringa oleifera Moringa oleifera aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. Improved growth, leaf photosynthetic pigments.
2. nutrient uptake.
3.Salt stress resistance.
4.Improved fresh and dry
biomass, antioxidants
activity
Enriched & Purified extract of Moringa oleifera 3.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and
quercetin 3-O-glucoside. 4.0 % 0.001-15 %
22. Eclipta alba Eclipta alba aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. Reduce pH of soil
2. Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
3. soil pH shifted toward neutrality
Enriched & Purified extract of Eclipta alba 3.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds ursolic acid, oleanolic acid. 4.0 % 0.001-15 %
23. Zingiber officinale Zingiber officinale aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. Reduce pH of soil
2. Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
3. soil pH shifted toward neutrality
4. enhancing the phytochemical and nutrient profiles.

Enriched & Purified extract of Zingiber officinale 3.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds Oxalic acid, Tartaric acid 4.0 % 0.001-15 %
24. Glycyrrhiza gabra Glycyrrhiza gabra aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. Improved vegetative growth, increased plant height, germination percentage, number of branches, Enhanced seedling growth,
photosynthetic attributes,
2. Stimulating root elongation
Enriched & Purified extract of Glycyrrhiza gabra 3.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin 4.0 % 0.001-15 %

25

Typha angustifolia Typha angustifolia aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. Increased values of osmotica (proline, total soluble sugars, K +, and P), chlorophyll and carotenoid content
Enriched & Purified extract of Typha angustifolia 3.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds sterols, terpenoids, flavones, and long-chain hydrocarbons 4.0 % 0.001-15 %
26. Euphorbia hirta Euphorbia hirta aqueous extract, solvent extract and hydroalcoholic extract, solvent extract and hydroalcoholic extract 12.0 % 0.001-35 % 1. To improve soil structure, promote soil aeration, soil water holding capacity, and plant nutrients.
2. Encouraging root extension, increasing the number of root points, and enhancing the length of the thin roots.
Enriched & Purified extract of Euphorbia hirta 3.0 % 0.001-25 %
Purified Phyto ingredients or marker compounds alkaloids, phenols, flavonoids, triterpenoids and tannins 4.0 % 0.001-15 %

Example 2: List of Ingredient used in formulation of botanical based soil conditioner along with their detailed properties.
Table 2

Sr. No. Botanicals Name Common name Part Used Active Ingredient (Marker compound) Soil conditioner / Bio-stimulant Properties
1. Sargassum wightii Gulfweed Whole plant part Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids , flavonoids, flavonols and flavonol glycosides 1. Enhancing the soil fertility
2. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
3. Suppressive effect on root pathogens of tomato and sunflower by reducing fungal root infection and nematode's galls on roots and nematode's penetration in roots.
2. Sargassum vulgare Gulfweed Whole plant part Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides 1. Enhancing the soil fertility
2. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
3. Increase root and shoot of plants
4. triggered early flowering and increased fruit set in crops, enhance plant defence system
3. Cladophora rupestris green branched weed Whole plant part Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides 1. Enhancing the soil fertility
2. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
3. Increase water holding capacity
4. triggered early flowering and increased fruit set in crops, enhance plant defence system
4. Ulva lactuca Sea lactuca Whole plant part Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides 1. Plant growth stimulators and enable the plants to withstand drought, disease or frost conditions
2. Increase water holding capacity
3. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients.
5. Ascophyllum nodosum Knotted Wrack. Whole plant part alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides
1. soil-conditioning properties due to alginic acid, improve water retention capacity of the soil, stimulate soil microbial activity.
2. Increase Phosphorus and sulphur uptake, increased biomass, protein content, chlorophyll and carotenoid content, flavonoids and phenolics, and increased antioxidant activity.
3.Improve plant Vigor, increase root development, enhance chlorophyll synthesis, promote earlier flowering, enhance fruit set and uniformity of fruit, reduce pod shatter, delay senescence.
4.Enhance tolerance to abiotic stress.

6. Caulerpa scalpelliformis Shy mermaid Whole plant part tannins, flavanoids, glycosides, phenols, saponins, terpenoids 1.Increasing the growth promoting hormones and nutrients, increased seed germination, increase lateral roots.
2. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients.

7. Kappaphycus alvarezii Sea moss Whole plant part ?-carrageenan, IAA, Cytokinins-Tz, GA3,tannins, flavanoids, glycosides, phenols, saponins, terpenoids 1. Increase Uptake of nutrients (Mg and Fe) and photosynthetic rate, promotion of overall vigor and the growth of plants.
2.Increased root length, enhanced chlorophyll content and carotenoids, and tissue water content under salinity and drought stress.
3.Accumulated Osmo protectants including proline, amino acids, and total protein

8. Macrocystis pyrifera Giant kelp Whole plant part Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids 1. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
2. Significant increase in flower number, inflorescence number, flower to fruit ratio, and increased fruit number and size, enhance plant defence system and increase in defense enzyme activity .
9. Acanthophora spicifera Spiny algae Whole plant part Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids 1. Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
2. Flowers, inflorescences, flower-to-fruit ratio, fruit size, fruit number, and defence enzyme activity all significantly increase, as does the plant's defence system and activity.
10. Ecklonia maxima Wild rice Aerial part Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids 1. Improve soil structure by boosting plant nutrients, aeration, and water retention.
2. Increase protein contain in crop, increase in root density,
3.Increases in plant growth and yield parameters as well as an
increase of protein and nutrient content.
11. Saccharina japonica Sweet kelp Whole plant part Organic matter, GA like compounds, Alginic acid and steroids 1.Increase soil aeration, increase water holding capacity, and add plant nutrients to strengthen soil structure.
2.Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
12. Arthrospira platensis Spirulina Whole plant part Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid 1. It enhanced total nitrogen and accessible phosphorus in the soil, improve soil nitrate levels
13.
Chlorella vulgaris Green alga Whole plant part Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl 1.It can be used as bio-fertilizer to enhance yield, improves the biological (CO2 evolution, dehydrogenase
activity, nitrogenase activity) and chemical
properties of the soil (pH, available-N, available-P, available-K)
14. Thuja occidentalis American arborvitae Aerial Part essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene 1. soil pH shifted toward neutrality, and substantial increments were observed in water holding capacity (WHC), cation exchange capacity (CEC), and N/P availability,
2.Improvements in leaf area index (LAI), leaf number, chlorophyll content, nitrate reductase (NR) activity.
15. Spinacia oleracea spinach Aerial Part phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids. 1. Deliver all major macro- and micronutrients to soil, improving the fertility.
2. Improve water holding capacity of soil.
16. Tribulus terrestis Gokshur Fruit flavonoids, flavonol glycosides, steroidal saponins, and alkaloids 1. Significant reduction of ammonia volatilization from soil
17. Citrus aurantium Bitter orange Fruit organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins 1. Reduce pH of soil.
2. release trap nutrient in soil and make it available for plants.
3.Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
18. Embelia ribes Vidanga Fruits Acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils 1. Reduce pH of soil.
2. release trap nutrient in soil and make it available for plants.
3.Improve soil structure through increasing water retention, increasing aeration and adding plant nutrients
19. Ficus benghalensis Banyan Aerial roots Flavonoids, phenols, terpenoids, and terpenes 1. Reduce pH of soil.
2.Improve soil structure.
20. Rosmarinus officinalis Rosemary Leave Rosmarinic acid 1. Reduce pH of soil
2. Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
21. Moringa oleifera Moringa Leaves and seeds Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and
quercetin 3-O-glucoside 1. Improved growth, leaf photosynthetic pigments.
2. nutrient uptake.
3.Salt stress resistance.
4.Improved fresh and dry
biomass, antioxidants
activity
22. Eclipta alba False daisy Aerial Part of plant ursolic acid, oleanolic acid 1. Reduce pH of soil
2. Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
3. soil pH shifted toward neutrality
23. Zingiber officinale Ginger Rhizome Oxalic acid, Tartaric acid 1. Reduce pH of soil
2. Stimulating root elongation, higher number of root tips, more pronounced length of thin roots.
3. soil pH shifted toward neutrality
4. enhancing the phytochemical and nutrient profiles.

24. Glycyrrhiza gabra Licorice Roots glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin 1. Improved vegetative growth, increased plant height, germination percentage, number of branches, Enhanced seedling growth,
photosynthetic attributes,
2. Stimulating root elongation
25. Typha angustifolia lesser bulrush Aerial Part of plant sterols, terpenoids, flavones, and long-chain hydrocarbons 1. Increased values of osmotica (proline, total soluble sugars, K +, and P), chlorophyll and carotenoid content
26. Euphorbia hirta asthma-plant Aerial Part of plant alkaloids, phenols, flavonoids, triterpenoids and tannins 1. To improve soil structure, promote soil aeration, soil water holding capacity, and plant nutrients.
2. encouraging root extension, increasing the number of root points, and enhancing the length of the thin roots.

Example 3: Composition of botanical based soil conditioner 1:
Table 3
Sr No. Ingredient More precise Percent Percent Range
1. Ascophyllum nodosum extract 2.5 % 0.01 to 25 %
2. Sargassum vulgare extract 2.5 % 0.01 to 20 %
3. Citrus aurantium extract 3.0 % 0.001 to 30 %
4. Embelia ribes extract 3.0 % 0.001 to 25 %
5. Thuja occidentalis extracts 3.0 % 0.001 to 35 %
6. Rosmarinus officinalis extracts 5.0 % 0.001 to 35 %
7. Bentonite Granules 81.0 % 60 to 95 %

Preparation of botanical based soil conditioner 1:
The process for preparing Botanical based Soil conditioner 1 comprises the steps of:

Ascophyllum nodosum, whole plant part was extracted by using Hydrophilic extraction using water as solvent. More precisely the entire extraction was carried between 30 degree celsius to 50 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography technique by using Two immiscible solvent methanol and hexane as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include alginates and fucoidan, laminarins, betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, Ga3 and glycosides etc.
Sargassum vulgare, whole plant part was extracted by using Hydrophilic extraction, by using various water as solvent. More precisely the entire extraction was carried between 30 degree celsius to 50 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography technique by using two immiscible solvent methanol and hexane as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides etc.
Citrus aurantium, dried fruits were extracted by using solvent extraction, by using methanol and acetone in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins.
Embelia ribes, dried fruits are extract by using solvent extraction, by using methanol and water in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils.
Thuja occidentalis, dried aerial parts were extracted by using solvent extraction, by using methanol and water in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene.
Rosmarinus officinalis, dried aerial parts are extract by using solvent extraction, by using methanol and acetone in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include Rosmarinus acid.
Extracts obtained in the above steps (steps 1 to 6) were blended at specific temperature ranging from 30°C to 45°C and stirring at specific RPM ranging from 50 -800 RPM.
Blend prepared in step no.7 was stirred at 600 to 1000 rpm at 35 to 45 degrees Celsius in closed mixing vessel made up of stainless steel 316 grade with outer jacket for maintaining temperature. The mixing vessel was connected with stirrer (blade stirrer, propeller stirrer, turbine stirrer, anchor stirrer, universal stirrer) for continues stirring towards the bottom of vessel homogenizer with the speed of 3700~27000 rpm was attached to reduce the particle size under very high pressures, sheer, turbulence, acceleration and impact, to make them more stable and effective.
Blend prepare in step no 8 was passed through in line shear pump with 1800 RPM to 5800 RPM to reduce particle size in nano, so as to achieve nano emulsion with particle size ranging from 10 to 1000 nano meter.
Further to obtain nano particles based formulation, the blend formed in step no 8 was further passed through High-pressure homogenization (homogenization at speed of 4000 rpm to 10000 rpm and pressure max up to 4,200 bar (60,000 psi)) to reduce the particle size below 100 nano meters.
After passing through high pressure homogenizer the material was passed through sparkler filter having seven layers of membrane filters with pore size less than 1-2 micron to achieve proper filtration.
Product recovered from step 11 was further quantified for the active phyto constituent by using various instruments but not limited to liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, High pressure liquid chromatograph, gas chromatography, spectroscopy compounds etc.
The product obtained in step no. 12 was loaded on carrier material consisting of bentonite powder or bentonite roasted granules by using high speed jet sprayer in presence of dry air at temp 40 degree celsius for easy drying.
The product obtained was also tested for bio-efficacy on different crops in vitro and in vivo, and it is compared to other soil conditioner.

Example 4: Composition of botanical based soil conditioner 2:
Table 4
Sr No. Ingredient More precise Percent Percent Range
1. Sargassum wightii extract 2.5 % 0.01 to 25 %
2. Ulva lactuca extract 2.0 % 0.01 to 20 %
3. Macrocystis pyrifera extract 2.0 % 0.01 to 20 %
4. Spinacia oleracea extract 2.0 % 0.001 to 30 %
5. Moringa oleifera extract 5.0 % 0.001 to 25 %
6. Glycyrrhiza gabra extracts 3.0 % 0.001 to 35 %
7. Rosmarinus officinalis extracts 5.0 % 0.001 to 35 %
8. Bentonite Granules 78.5 % 60 to 95 %

Preparation of botanical based soil conditioner 2:
The process for preparing the Botanical based Soil conditioner 2 comprises the steps of:

Sargassum wightii, whole plant part was extracted by using Hydrophilic extraction, by using water as solvent. More precisely the entire extraction was carried between 30 degree celsius to 50 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtained extract was performed by using liquid-liquid chromatography by using Two immiscible solvent methanol and hexane as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids, flavonoids, flavonols and flavonol glycosides etc.
Ulva lactuca, whole plant part was extracted by using Hydrophilic extraction, by using water as solvent. More precisely the entire extraction was carried between 30 degree celsius to 50 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtained extract was performed by using liquid-liquid chromatography by using Two immiscible solvent methanol and hexane as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides etc.
Macrocystis pyrifera, whole plant part was extracted by using Hydrophilic extraction, by using water as solvent. More precisely the entire extraction was carried between 30 degree celsius to 50 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtained extract was performed by using liquid-liquid chromatography by using Two immiscible solvent methanol and hexane as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids etc.
Spinacia oleracea, dried aerial parts were extracted by using solvent extraction, by using methanol and acetone in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids.
Moringa oleifera, dried aerial parts were extract by using solvent extraction, by using methanol and water in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtained extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and quercetin 3-O-glucoside.
Glycyrrhiza gabra, dried aerial parts were extracted by using solvent extraction, by using methanol and water in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin.
Rosmarinus officinalis, dried aerial parts are extract by using solvent extraction, by using methanol and acetone in combination (1:1) as solvent. More precisely the entire extraction was carried between 40 degree celsius to 60 degree celsius under vacuum in accordance to preserve heat sensitive molecules. Further enrichment and purification of above obtain extract was performed by using liquid-liquid chromatography by using two immiscible solvents as Purification medium followed by precipitation and crystallisation etc. to obtain purified Phyto ingredients which include Rosmarinus acid.

, Claims:1. A botanical based soil conditioner with bio-stimulant activity, for improving soil health, comprising:
i. Sargassum wightii extract or Enriched and Purified extract of Sargassum wightii or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids, flavonoids, flavonols and flavonol glycosides extracted from said botanical source at a concentration in a range of 0.001-25 %, more preferably 1.0 - 6.5 %;
ii. Sargassum vulgare extract or Enriched and Purified extract of Sargassum vulgare or Purified Phyto ingredients or marker compounds such Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides extracted from said botanical source at a concentration in a range of 0.001-20 %, more preferably 0.5 - 6.5 %;
iii. Cladophora rupestris extract or Enriched and Purified extract of Cladophora rupestris or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides extracted from said botanical source at a concentration in a range of 0.001-25 %, more preferably 0.5 - 6.5 %;
iv. Ulva lactuca extract or enriched and purified extract of Allium sativum or Purified Phyto ingredients or marker compounds such as Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source at a concentration in a range of 0.001-20 %, more preferably 0.01- 4.5;
v. Ascophyllum nodosum extract or Enriched and Purified extract of Ascophyllum nodosum or Purified Phyto ingredients or marker compounds such as alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source at a concentration in a range between 0.01-25 %; more preferably 0.5- 4.5 %;
vi. Caulerpa scalpelliformis extract or Enriched and Purified extract of Caulerpa scalpelliformis or Purified Phyto ingredients or marker compounds such as tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source at a concentration in a range of 0.001-20 %, more preferably 0.5 - 6.5 %;
vii. Kappaphycus alvarezii extract or Enriched and Purified extract of Kappaphycus alvarezii or Purified Phyto ingredients or marker compounds such as ?-carrageenan, IAA, Cytokinins-Tz, GA3, tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source at a concentration in a range of 0.001-20 %, more preferably 2.5 - 8.5 %;
viii. Macrocystis pyrifera extract or Enriched and Purified extract of Macrocystis pyrifera or Purified Phyto ingredients or marker compounds such as Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001-20 % more preferably 0.5 - 6.5 %;
ix. Acanthophora spicifera extract or Enriched and Purified extract of Acanthophora spicifera or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001-20 %, more preferably 0.5 - 6.5 %;
x. Ecklonia maxima extract or Enriched and Purified extract of Ecklonia maxima or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source at a concentration in a range of 0.001-20 %, more preferably 0.5 - 6.5 %;
xi. Saccharina japonica extract or Enriched and Purified extract of Saccharina japonica or Purified Phyto ingredients or marker compounds such as Organic matter, GA like compounds, Alginic acid and steroids extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
xii. Arthrospira platensis extract or Enriched and Purified extract of Arthrospira platensis or Purified Phyto ingredients or marker compounds such as Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
xiii. Chlorella vulgaris extract or Enriched and Purified extract of Chlorella vulgaris or Purified Phyto ingredients or marker compounds such as Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl extracted from said botanical source at a concentration in a range of 0.001-20 %, more preferably 0.5 - 6.5 %;
xiv. Thuja occidentalis extract or Enriched and Purified extract of Thuja occidentalis or Purified Phyto ingredients or marker compounds such as essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 2.5 - 8.5 %;
xv. Spinacia oleracea extract or Enriched and Purified extract of Spinacia oleracea or Purified Phyto ingredients or marker compounds such as phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
xvi. Tribulus terrestis extract or Enriched and Purified extract of Tribulus terrestis or Purified Phyto ingredients or marker compounds such as flavonoids, flavonol glycosides, steroidal saponins, and alkaloids extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
xvii. Citrus aurantium extract or Enriched and Purified extract of Citrus aurantium or Purified Phyto ingredients or marker compounds such as organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 1 - 6.5 %;
xviii. Embelia ribes extract or Enriched and Purified extract of Embelia ribes or Purified Phyto ingredients or marker compounds such as acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils extracted from said botanical source at a concentration in a range of 0.001-25 %, more preferably 1 - 6.5 %;
xix. Ficus benghalensis extract or Enriched and Purified extract of Ficus benghalensis or Purified Phyto ingredients or marker compounds such as acidic compounds lavonoids, phenols, terpenoids, and terpenes extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 0.5 - 6.5 %;
xx. Rosmarinus officinalis extract or Enriched and Purified extract of Rosmarinus officinalis or Purified Phyto ingredients or marker compounds such Rosmarinic acid extracted from said botanical source at a concentration in a range of 0.001-35%, more preferably 2.5 - 8.5%;
xxi. Moringa oleifera extract or Enriched and Purified extract of Moringa oleifera or Purified Phyto ingredients or marker compounds such as Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and quercetin 3-O-glucoside extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %;
xxii. Eclipta alba extract or Enriched and Purified extract of Eclipta alba or Purified Phyto ingredients or marker compounds such as ursolic acid and oleanolic acid extracted from said botanical source at a concentration in a range of 0.001-30 %, more preferably 0.5 - 6.5 %;
xxiii. Zingiber officinale extract or Enriched and Purified extract of Zingiber officinale or Purified Phyto ingredients or marker compounds such as Oxalic acid and Tartaric acid extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 8.5 % ;
xxiv. Glycyrrhiza gabra extract or Enriched and Purified extract of Glycyrrhiza gabra or Purified Phyto ingredients or marker compounds such as glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %;
xxv. Typha angustifolia extract or Enriched and Purified extract of Typha angustifolia or Purified Phyto ingredients or marker compounds such as sterols, terpenoids, flavones, and long-chain hydrocarbons extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 0.5 - 6.5 %;
xxvi. Euphorbia hirta extract or Enriched and Purified extract of Euphorbia hirta or Purified Phyto ingredients or marker compounds such as alkaloids, phenols, flavonoids, triterpenoids and tannins extracted from said botanical source at a concentration in a range of 0.001-35 %, more preferably 1 - 6.5 %; and
xxvii. carrier material at a concentration in a range of 60-95 %; more preferably 75-95 %;
2. The soil conditioner, as claimed in Claim 1, wherein said carrier material is selected from the group consisting of bentonite powder, bentonite roasted granules and the like.
3. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises combination of standardised enriched botanical extract and purified phyto ingredients.
4. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner consists of enriched and purified Phyto ingredients selected from Organic matter, Humic acid, saponins, polyphenols, alkaloids, flavonoids, proteins terpenoids, or secondary metabolites, other plant growth promoting substances from botanicals.
5. The soil conditioner, as claimed in Claim 4, wherein said soil conditioner consists of enriched and purified Phyto ingredients selected from IBA, IAA, GA3, Endogenous Salicylic acid, ulvan, carotenoid tannins, flavanoids, glycosides, phenols, saponins, terpenoids sterols, terpenoids, flavonoids, glycosides, alkaloids and carbohydrates alginates and fucoidan, laminarins, Betaines, glycinebetaine, polyphenols eckol, phloroglucinol, polyamines, GA, castasterone, brassinolide, Polysaccharides Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin Kaempferol 3-O-glucoside, isorhamnetin 3-O-galactoside, rutin, phenylalanine and tryptophan ursolic acid, oleanolic acid Oxalic acid, Tartaric acid glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin rosmaric acid, ascorbic acid, quercetin 3-O-glucoside.
6. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Ascophyllum nodosum extract in the range of 0.01 to 25 %;
ii. Sargassum vulgare extract in the range of 0.01 to 20 %;
iii. Citrus aurantium extract in the range of 0.001 to 30 %;
iv. Embelia ribes in the range of 0.001 to 25 %;
v. Thuja occidentalis extract in the range of 0.001 to 35 %;
vi. Rosmarinus officinalis extracts in the range of 0.001 to 35 %; and
vii. Bentonite Granules in the range of 60 to 95 %
7. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Sargassum wightii extract in the range of 0.01 to 25 %;
ii. Ulva lactuca extract in the range of 0.01 to 20 %;
iii. Macrocystis pyrifera extract in the range of 0.01 to 20 %;
iv. Spinacia oleracea extract in the range of 0.001 to 30 %;
v. Moringa oleifera extract in the range of 0.001 to 25 %;
vi. Glycyrrhiza gabra extract in the range of 0.001 to 35 %;
vii. Rosmarinus officinalis extracts in the range of 0.001 to 35 %; and
viii. Bentonite Granules in the range of 60 to 95 %
8. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Cladophora rupestris extract in the range of 0.01 to 25 %;
ii. Ulva lactuca extract in the range of 0.01 to 20 %;
iii. Kappaphycus alvarezii extract in the range of 0.01 to 20 %;
iv. Eclipta alba extract in the range of 0.001 to 30 %;
v. Moringa oleifera extract in the range of 0.001 to 25 %;
vi. Typha angustifolia extract in the range of 0.001 to 35 %;
vii. Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
viii. Bentonite Granules in the range of 60 to 95 %
9. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Sargassum wightii extract in the range of 0.01 to 25 %;
ii. Saccharina japonica extract in the range of 0.01 to 20 %;
iii. Chlorella vulgaris extract in the range of 0.01 to 20 %;
iv. Tribulus terrestis extract in the range of 0.001 to 30 %;
v. Moringa oleifera extract in the range of 0.001 to 25 %;
vi. Zingiber officinale extract in the range of 0.001 to 35 %;
vii. Ficus benghalensis extracts in the range of 0.001 to 35 %; and
viii. Bentonite Granules in the range of 60 to 95 %
10. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Sargassum wightii extract in the range of 0.01 to 25 %;
ii. Caulerpa scalpelliformis extract in the range of 0.01 to 20 %;
iii. Acanthophora spicifera extract in the range of 0.01 to 20 %;
iv. Spinacia oleracea extract in the range of 0.001 to 30 %;
v. Moringa oleifera extract in the range of 0.001 to 25 %;
vi. Euphorbia hirta extract in the range of 0.001 to 35 %;
vii. Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
viii. Bentonite Granules in the range of 60 to 95 %
11. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Sargassum wightii extract in the range of 0.01 to 25 %;
ii. Ulva lactuca extract in the range of 0.01 to 20 %;
iii. Ecklonia maxima extract in the range of 0.01 to 20 %;
iv. Arthrospira platensis extract in the range of 0.001 to 30 %;
v. Moringa oleifera extract in the range of 0.001 to 25 %;
vi. Glycyrrhiza gabra extract in the range of 0.001 to 35 %;
vii. Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
viii. Bentonite Granules in the range of 60 to 95 %
12. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Ascophyllum nodosum extract in the range of 0.01 to 25 %;
ii. Sargassum vulgare extract in the range of 0.01 to 20 %;
iii. Citrus aurantium extract in the range of 0.001 to 30 %;
iv. Embelia ribes extract in the range of 0.001 to 25 %;
v. Moringa oleifera extract in the range of 0.001 to 35 %;
vi. Rosmarinus officinalis extract in the range of 0.001 to 35 %; and
vii. Bentonite Granules in the range of 60 to 95 %
13. The soil conditioner, as claimed in Claim 1, wherein said soil conditioner comprises:
i. Ascophyllum nodosum extract in the range of 0.01 to 25 %;
ii. Ulva lactuca extract in the range of 0.01 to 20 %;
iii. Citrus aurantium extract in the range of 0.001 to 30 %;
iv. Embelia ribes extract in the range of 0.001 to 25 %;
v. Moringa oleifera extract in the range of 0.001 to 35 %;
vi. Thuja occidentalis extract in the range of 0.001 to 35 %; and
vii. Bentonite Granules in the range of 60 to 95 %
14. A process for preparation of the botanical based soil conditioner with bio-stimulant activity, comprising:
a. Extracting 0.001- 25 % of Sargassum wightii extract or Enriched and Purified extract of Sargassum wightii or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Alginate fucoidan and fucoxanthin, polyphenols, phloroglucinol, phlorotannins, phenolic acids , flavonoids, flavonols and flavonol glycosides extracted from said botanical source; 0.001- 20 % of Sargassum vulgare extract or Enriched and Purified extract of Sargassum vulgare or Purified Phyto ingredients or marker compounds such Organic matter, Humic acid, Alginate Heptacosane, Oleic Acid and other plant growth promoting substances such as IAA and IBA, Gibberellins (A&B), cytokinins, micronutrients, vitamins, amino acids glycosides extracted from said botanical source; 0.001- 25 % of Cladophora rupestris extract or Enriched and Purified extract of Cladophora rupestris or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, Lipids, alkaloids flavonoids proteins and glycosides extracted from said botanical source; 0.001- 20 % of Ulva lactuca extract or enriched and purified extract of Ulva lactuca or Purified Phyto ingredients or marker compounds such as Ulvan, carotenoid, Organic matter, Humic acid, Lipids, alkaloids flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source; 0.01 – 25 % of Ascophyllum nodosum extract or Enriched and Purified extract of Ascophyllum nodosum or Purified Phyto ingredients or marker compounds such as alginates and fucoidan, laminarins, Betaines, glycine betaine, polyphenols flavonoids, proteins, IBA, IAA, GA3 and glycosides extracted from said botanical source; 0.001- 20 % of Caulerpa scalpelliformis extract or Enriched and Purified extract of Caulerpa scalpelliformis or Purified Phyto ingredients or marker compounds such as tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source; 0.001- 20 % of Kappaphycus alvarezii extract or Enriched and Purified extract of Kappaphycus alvarezii or Purified Phyto ingredients or marker compounds such as ?-carrageenan, IAA, Cytokinins-Tz, GA3,tannins, flavanoids, glycosides, phenols, saponins, terpenoids extracted from said botanical source; 0.001- 20 % of Macrocystis pyrifera extract or Enriched and Purified extract of Macrocystis pyrifera or Purified Phyto ingredients or marker compounds such as Alginic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 20 % of Acanthophora spicifera extract or Enriched and Purified extract of Acanthophora spicifera or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 20 % of Ecklonia maxima extract or Enriched and Purified extract of Ecklonia maxima or Purified Phyto ingredients or marker compounds such as Organic matter, Humic acid, saponins, phenols, flavonoids, alkaloids and steroids extracted from said botanical source; 0.001- 30 % of Saccharina japonica extract or Enriched and Purified extract of Saccharina japonica or Purified Phyto ingredients or marker compounds such as Organic matter, GA like compounds, Alginic acid and steroids extracted from said botanical source; 0.001- 30 % of Arthrospira platensis extract or Enriched and Purified extract of Arthrospira platensis or Purified Phyto ingredients or marker compounds such as Organic matter, phycocyanin, sulfated polysaccharides, and ?-linolenic acid extracted from said botanical source; 0.001- 20 % of Chlorella vulgaris extract or Enriched and Purified extract of Chlorella vulgaris or Purified Phyto ingredients or marker compounds such as Organic matter, cellulose, hemicellulose, monosaccharides, lipid, protein, and glycosyl extracted from said botanical source; 0.001-35 % of Thuja occidentalis extract or Enriched and Purified extract of Thuja occidentalis or Purified Phyto ingredients or marker compounds such as essential oil, reducing sugars, polysaccharides, minerals, free acids, and tannic agents thujone, isothujone, fenchone, sabines, and a-pinene extracted from said botanical source; 0.001- 30 % of Spinacia oleracea extract or Enriched and Purified extract of Spinacia oleracea or Purified Phyto ingredients or marker compounds such as phytosterols, proteins, glycosides, flavonoids, carbohydrates, quinones, and terpenoids extracted from said botanical source; 0.001- 30 % of Tribulus terrestis extract or Enriched and Purified extract of Tribulus terrestis or Purified Phyto ingredients or marker compounds such as flavonoids, flavonol glycosides, steroidal saponins, and alkaloids extracted from said botanical source; 0.001- 30 % of Citrus aurantium extract or Enriched and Purified extract of Citrus aurantium or Purified Phyto ingredients or marker compounds such as organic acid, citric acid, steroids, flavonoids, saponins, alkaloids, phenols and tannins extracted from said botanical source; 0.001- 25 % of Embelia ribes extract or Enriched and Purified extract of Embelia ribes or Purified Phyto ingredients or marker compounds such as acidic compounds, embelin, quercitol, fatty ingredients and alkaloid schristembine, a resinoid, tannins and volatile oils extracted from said botanical source; 0.001-35 % of Ficus benghalensis extract or Enriched and Purified extract of Ficus benghalensis or Purified Phyto ingredients or marker compounds such as acidic compounds lavonoids, phenols, terpenoids, and terpenes extracted from said botanical source; 0.001-35 % of Rosmarinus officinalis extract or Enriched and Purified extract of Rosmarinus officinalis or Purified Phyto ingredients or marker compounds such Rosmarinic acid extracted from said botanical source; 0.001-35 % of Moringa oleifera extract or Enriched and Purified extract of Moringa oleifera or Purified Phyto ingredients or marker compounds such as Syringic acid, gallic acid, Kaempferol 3-O-rhamnoside, rutin and quercetin 3-O-glucoside extracted from said botanical source; 0.001- 30 % of Eclipta alba extract or Enriched and Purified extract of Eclipta alba or Purified Phyto ingredients or marker compounds such as ursolic acid and oleanolic acid extracted from said botanical source; 0.001-35 % of Zingiber officinale extract or Enriched and Purified extract of Zingiber officinale or Purified Phyto ingredients or marker compounds such as Oxalic acid and Tartaric acid extracted from said botanical source; 0.001-35 % of Glycyrrhiza gabra extract or Enriched and Purified extract of Glycyrrhiza gabra or Purified Phyto ingredients or marker compounds such as glabrin, isoflavones, glabridin, hispaglabridin A, and 30-hydroxy-4-O-methylglabridin extracted from said botanical source; 0.001-35 % of Typha angustifolia extract or Enriched and Purified extract of Typha angustifolia or Purified Phyto ingredients or marker compounds such as sterols, terpenoids, flavones, and long-chain hydrocarbons extracted from said botanical source; 0.001-35 % of Euphorbia hirta extract or Enriched and Purified extract of Euphorbia hirta or Purified Phyto ingredients or marker compounds such as alkaloids, phenols, flavonoids, triterpenoids and tannins extracted from said botanical source;
b. blending the above extracts at specific temperature ranging from 30°C to 45°C and stirring at specific RPM ranging from 50 -800 RPM;
c. stirring the mixture obtained in step (b) at 600 to 1000 rpm RPM at 35-45°C in closed mixing vessel for continued stirring followed by homogenizing the mix with the homogenizer fitted towards the bottom of vessel and with the speed of 3700~27000 rpm to reduce the particle size.;
d. passing the homogenized mixture obtained in step (c) through in line shear pump with 1800 RPM to 5800 RPM to reduce particle size to obtain the nano emulsion with particle size ranging from 10 to 1000 nano meter;
e. passing the mixture obtained in step (d) through High-pressure homogenization to reduce the particle size below 100 nano meters;
f. passing the mixture obtained in step (g) through sparkler filter having seven layers of membrane filters with pore size less than 1-2 micron to achieve desired filtration;
g. loading the product obtained in step (f) on carrier material by using high speed jet sprayer in presence of dry air at temp 40 degree celsius for easy drying; and
h. recovering the final product.
15. The process as claimed in Claim 14, wherein extraction process of step (a) comprises enrichment and purification of the extract using techniques which include but not limited to liquid-liquid extraction based on polarity of phyto ingredient, chromatography, precipitation and crystallisation to obtain purified phyto ingredients.
16. The process as claimed in Claim 14, wherein the the homogenization of step (c) is done under very high pressures, sheer, turbulence, acceleration and impact, to make the particles more stable and effective.
17. The process as claimed in Claim 14, wherein high-pressure homogenization of step (e) is carried out at a speed of 4000 rpm to 10000 rpm and pressure up to 4,200 bar (60,000 psi).
18. The process as claimed in Claim 14, wherein said extraction techniques include but not limited to hydrophilic extraction, super critical carbon dioxide extraction, steam distillation, solvent extraction, Soxhlet extraction and the like.
19. The process as claimed in Claim 18, wherein said extraction technique is selected from the group consisting of Solvent extraction, steam distillation and super critical carbon dioxide extraction and the like.
20. The process as claimed in Claim 14, wherein said carrier material is selected from the group comprising bentonite powder, bentonite roasted granules and the like.
21. The process as claimed in Claim 14, wherein said carrier material is at a concentration in a range of 60-95 %.
22. The process as claimed in Claim 21, wherein said carrier material is more preferably at a concentration in a range of 75-95 %.
23. The process as claimed in Claim 14, wherein the solvent used in the solvent extraction includes but not limited to water, alcohols, hydrocarbons, ketones, esters, ethers, and the like alone or in combination thereof.
24. The process as claimed in Claim 23, wherein the solvent used in the solvent extraction is selected from the group comprising water, ethanol, methanol, ethyl acetate, hexane, acetone and the like.
25. The process as claimed in Claim 14, wherein further purification of Phyto ingredient is carried out by using liquid liquid extraction, various chromatograph techniques like liquid-liquid chromatography, column chromatography, affinity chromatography, silica gel chromatography, ion exchange chromatography and other purification like precipitation and crystallization.
26. The process as claimed in Claim 14, wherein the standardized botanical extract are blended with each other at temperature ranging from 30oC to 45oC and stirring at specific RPM ranging from 50 -800 RPM.
27. The process as claimed in Claim 14, wherein the particle size of active blend is reduced to nano range by using various modules like Filter press, shear pump, homogenizer and High pressure homogenization (pressure max up to 45,000 PSI) obtain Nano formulation with average particle size range between 10 -1000 nm.
28. The process as claimed in Claim 14, wherein the the active blend form is loaded on carrier material selected from the group comprising bentonite powder or bentonite granules and the like by using high speed jet sprayer.
29. The soil conditioner, as claimed in any one of the preceding Claims, wherein said soil conditioner is effective against nematodes including but not limited to Root knot nematodes (meloidogyne spp.), cyst nematodes (Heterodera and Globoders spp.), Root lesion nematodes (pratylenchus spp.) and fungal plant pathogens including but not limited to Armillaria mellea, Clitocybe tabescens, and Fusarium, and many oomycetes, including Pythium, Phytophthora, and Aphanomyces and the like in plants.
30. The soil conditioner, as claimed in any one of the preceding Claims, wherein said soil conditioner is at a concentration ranging between 4-8 kg per acer or 10 – 20 kg per hectar more precise 8 kg per acer or 18 kg per hectar.
31. The soil conditioner, as claimed in any one of the preceding Claims, wherein said soil conditioner may be applied by sprinkler application, sprayer application or drip application, more preferably by sprayer application such as foliar sprays, sprays to be applied to plants shoots and the like.
32. The soil conditioner, as claimed in any one of the preceding Claims, wherein said soil conditioner is useful for improving soil health and nourishing growth of beneficial organisms in soil, providing critical nutrients and minerals to plants, boosting plant germination, improving yield of crops, protecting plants and increasing plant resistance to pests and diseases.
33. The soil conditioner, as claimed in any one of the preceding Claims, wherein said soil conditioner improves soil health, exhibits biostimulant activity and thereby improves plant growth and crop yield.
34. The soil conditioner, as claimed in any one of the preceding Claims, wherein the soil conditioner may be in the form of liquid, paste, powder or powder converted into granule or tablet form and the like.
35. The soil conditioner, as claimed in Claim 34, wherein the soil conditioner may be in liquid form.
36. A method for improving soil healthy and promoting the growth of the plants comprising applying the present soil conditioner to the soil or to the plant as a basal treatment and top dressing in an effective amount.