Method For Expansion And Maintenance Of Nk Cells For Immuno Therapy

Abstract: ABSTRACT METHOD FOR EXPANSION AND MAINTENANCE OF NK CELLS FOR IMMUNO-THERAPY The present invention discloses a method for expansion of natural killer (NK) cells 5 or CAR-NK cells using peripheral blood mononuclear cells (PBMCs) and culturing the cells in a medium comprising a feeder layer having a population of mesenchymal stem cells (MSCs) with disrupted or deleted MHC class I gene and/or MHC class II gene and a heterologous gene for expressing Interleukin-21(IL-21), and Interleukin-2 (IL-2). The present disclosure further provides a method and a 10 model for evaluating natural killer (NK) cells or CAR-NK cells using tester line comprising induced Pluripotent stem cells (iPSCs) comprising disrupted or deleted MHC class I gene and/or MHC class II gene and a heterologous gene encoding a tumor antigen. 15 46

Patent Information

Application #

Filing Date

14 June 2023

Publication Number

51/2024

Publication Type

INA

Invention Field

BIOTECHNOLOGY

Status

Email

Parent Application

Applicants

NKURE THERAPEUTICS PRIVATE LIMITED

Nkure Therapeutics Private Limited, Desk No. 15-L, No. 14, Bhattarahalli, Old Madras Road, Bangalore- 560049, India

Inventors

1. ROY, Rohini

Nkure Therapeutics Private Limited Desk No. 15-L, No. 14, Bhattarahalli, Old Madras Road, Bangalore- 560049, India

2. JUNEJA SHAH, Vandya

Nkure Therapeutics Private Limited Desk No. 15-L, No. 14, Bhattarahalli, Old Madras Road, Bangalore- 560049, India

3. BATTU, Aditi Ravindra

Nkure Therapeutics Private Limited Desk No. 15-L, No. 14, Bhattarahalli, Old Madras Road, Bangalore- 560049, India

Specification

DESC:METHOD FOR EXPANSION AND MAINTENANCE OF NK CELLS FOR IMMUNO-THERAPY ,CLAIMS:AS ATTACHED

Documents

Application Documents

#	Name	Date
1	202341040645-STATEMENT OF UNDERTAKING (FORM 3) [14-06-2023(online)].pdf	2023-06-14
2	202341040645-PROVISIONAL SPECIFICATION [14-06-2023(online)].pdf	2023-06-14
3	202341040645-FORM FOR STARTUP [14-06-2023(online)].pdf	2023-06-14
4	202341040645-FORM FOR SMALL ENTITY(FORM-28) [14-06-2023(online)].pdf	2023-06-14
5	202341040645-FORM 1 [14-06-2023(online)].pdf	2023-06-14
6	202341040645-EVIDENCE FOR REGISTRATION UNDER SSI(FORM-28) [14-06-2023(online)].pdf	2023-06-14
7	202341040645-DRAWINGS [14-06-2023(online)].pdf	2023-06-14
8	202341040645-FORM-26 [26-07-2023(online)].pdf	2023-07-26
9	202341040645-RELEVANT DOCUMENTS [08-02-2024(online)].pdf	2024-02-08
10	202341040645-Proof of Right [08-02-2024(online)].pdf	2024-02-08
11	202341040645-PETITION UNDER RULE 137 [08-02-2024(online)].pdf	2024-02-08
13	202341040645-Sequence Listing in PDF [12-06-2024(online)].pdf	2024-06-12
14	202341040645-DRAWING [12-06-2024(online)].pdf	2024-06-12
15	202341040645-CORRESPONDENCE-OTHERS [12-06-2024(online)].pdf	2024-06-12
16	202341040645-COMPLETE SPECIFICATION [12-06-2024(online)].pdf	2024-06-12
17	202341040645-Request Letter-Correspondence [13-06-2024(online)].pdf	2024-06-13
18	202341040645-Power of Attorney [13-06-2024(online)].pdf	2024-06-13
19	202341040645-Form 1 (Submitted on date of filing) [13-06-2024(online)].pdf	2024-06-13
20	202341040645-Covering Letter [13-06-2024(online)].pdf	2024-06-13
21	202341040645-CERTIFIED COPIES TRANSMISSION TO IB [13-06-2024(online)].pdf	2024-06-13
22	202341040645-ENDORSEMENT BY INVENTORS [26-06-2024(online)].pdf	2024-06-26
23	202341040645-FORM 18 [25-08-2025(online)].pdf	2025-08-25