Abstract: The present disclosure provides a skin care composition comprising apple leaf callus extract, and nicotinamide, which at particular w/w ratios enhances collagen secretion, which is a marker for healthy and firm skin.
Claims:1. A composition comprising:
a. apple leaf callus extract; and
b. nicotinamide,
wherein apple leaf callus extract to nicotinamide w/w ratio in said composition is in the range of 1:0.14-1:1.33.
2. The composition as claimed in claim 1, wherein apple leaf callus extract weight percentage in said composition is in the range of 0.003-0.007%, and nicotinamide weight percentage in said composition is in the range of 0.001-0.004%.
3. The composition as claimed in claim 1, wherein apple leaf callus extract to nicotinamide w/w ratio in said composition is 1:0.5.
4. The composition as claimed in claim 3, wherein apple leaf callus extract weight percentage in said composition is 0.005%, and nicotinamide weight percentage in said composition is 0.0025%.
5. The composition as claimed in any of the claims 1-4, further comprising suitable carriers, diluents, and excipients.
6. The composition as claimed in any of the claims 1-4, wherein said apple leaf callus extract is prepared by:
a. obtaining callus mass developed from apple leaf explants;
b. processing said callus mass into a paste and contacting said paste with at least one preservative at a weight concentration in the range of 0.2-0.4%, and at least one anti-oxidant at a weight concentration in the range of 0.1-0.3% to obtain a second paste;
c. contacting said second paste with methanol for 10-15 hours and constant stirring, followed by filtration to obtain a retentate;
d. contacting said retentate with dichloromethane for 10-15 hours and constant stirring, followed by filtration to obtain a filtrate; and
e. drying said filtrate to obtain said apple leaf callus extract.
7. A method of preparing a composition as claimed in any of the claims 1-6.
8. A method of inhibiting appearance of skin aging, said method comprising:
a. obtaining a composition as claimed in any of the claims 1-6; and
b. contacting said composition with skin,
wherein said method inhibits appearance of skin aging.
9. A composition as claimed in any of the claims 1-6 for use in preparing topical formulations to inhibiting appearance of skin aging.
, Description:As Attached
| # | Name | Date |
|---|---|---|
| 1 | Form 5 [30-03-2016(online)].pdf | 2016-03-30 |
| 2 | Form 3 [30-03-2016(online)].pdf | 2016-03-30 |
| 3 | Drawing [30-03-2016(online)].pdf | 2016-03-30 |
| 4 | Description(Complete) [30-03-2016(online)].pdf | 2016-03-30 |
| 5 | Other Patent Document [20-05-2016(online)].pdf | 2016-05-20 |
| 6 | Form 26 [20-05-2016(online)].pdf | 2016-05-20 |
| 7 | Other Patent Document [22-06-2016(online)].pdf | 2016-06-22 |
| 8 | Form 26 [22-06-2016(online)].pdf | 2016-06-22 |
| 9 | 201641011207-Power of Attorney-270616.pdf | 2016-07-29 |
| 10 | 201641011207-Form 1-270616.pdf | 2016-07-29 |
| 11 | 201641011207-Correspondence-PA-270616.pdf | 2016-07-29 |
| 12 | 201641011207-FORM 18 [27-03-2020(online)].pdf | 2020-03-27 |
| 13 | 201641011207-FER.pdf | 2021-10-17 |
| 14 | 201641011207-FER_SER_REPLY [07-02-2022(online)].pdf | 2022-02-07 |
| 15 | 201641011207-US(14)-HearingNotice-(HearingDate-24-06-2022).pdf | 2022-05-20 |
| 16 | 201641011207-Correspondence to notify the Controller [22-06-2022(online)].pdf | 2022-06-22 |
| 17 | 201641011207-Written submissions and relevant documents [08-07-2022(online)].pdf | 2022-07-08 |
| 18 | 201641011207-US(14)-HearingNotice-(HearingDate-15-06-2023).pdf | 2023-05-23 |
| 19 | 201641011207-Correspondence to notify the Controller [09-06-2023(online)].pdf | 2023-06-09 |
| 20 | 201641011207-FORM-26 [13-06-2023(online)].pdf | 2023-06-13 |
| 21 | 201641011207-Written submissions and relevant documents [27-06-2023(online)].pdf | 2023-06-27 |
| 22 | 201641011207-PatentCertificate28-06-2023.pdf | 2023-06-28 |
| 23 | 201641011207-IntimationOfGrant28-06-2023.pdf | 2023-06-28 |
| 1 | SearchHistory(8)E_19-08-2021.pdf |