TECHNICAL FIELD
[001] The subject matter described herein in general relates to the pharmaceutical and food supplement compositions, and in particular, relates to the field of herbal compositions.
BACKGROUND OF INVENTION
[002] Iron is an important mineral for human health. Iron helps in the physical and mental growth of humans and the requirement increases with age. Recent survey revealed that iron deficiency is most prevalent in India (Rekha Kumari et.al, J Clin Diagn Res. 2017). Some of the dietary factors are known to impair the nonheme iron absorption (Richard Hurrell et. al., Am J Clin Nutr 2010). Therefore, the iron bioavailability is found to be low in our diet. To overcome this problem, first approach is that people can take iron supplements. However, daily intake of iron supplementation has some known side effects such as gastrointestinal problems (Zoe Tolkien et.al, PLoS One. 2015). US7585527 discloses the usage of iron polysaccharides as an effective iron supplement. EP2661273 discloses an iron chelate as a food supplement.
[003] The second approach involves taking some adjuvants which have the ability to improve the non-heme iron absorption from regular dietary intake. The usage of herbal extracts in this approach may be particularly advantageous as it is known to have low cellular toxicity. WO2006068729 discloses a combination of two organic acids for enhancing iron-uptake. SUMMARY OF THE INVENTION
[004] In an aspect of the present disclosure, there is provides a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1.
[005] In another aspect of the present disclosure, there is provided a process for preparing the herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1, said process

comprising steps of: a) obtaining Zingiber officinale extract; b) at least one organic
compound; and c) contacting the Zingiber officinale extract with at least one
organic compound to obtain the composition.
[006] These and other features, aspects, and advantages of the present subject
matter will be better understood with reference to the following description and
appended claims. This summary is provided to introduce a selection of concepts in
a simplified form. This summary is not intended to identify key features or
essential features of the claimed subject matter, nor is it intended to be used to limit
the scope of the claimed subject matter.
BRIEF DESCRIPTION OF THE DRAWINGS
[007] The detailed description is described with reference to the accompanying
figures. The same numbers are used throughout the drawings to reference like
features and components.
[008] Figure 1 illustrates the evaluation of different actives for iron absorption on
Caco2 cells in the presence of FeSC>4 (lOuM), in accordance with an
implementation of the present subject matter.
[009] Figure 2 illustrates the evaluation of selected combinations for iron
absorption on Caco2 cells in the presence of FeS04 (lOuM), in accordance with an
implementation of the present subject matter.
[0010] Figure 3 illustrates the evaluation of different ratios of selected combination
for iron absorption on Caco2 cells in the presence of FeSC>4 (lOuM), in accordance
with an implementation of the present subject matter.
DETAILED DESCRIPTION OF THE INVENTION
[0011] Those skilled in the art will be aware that the present disclosure is subject to
variations and modifications other than those specifically described. It is to be
understood that the present disclosure includes all such variations and
modifications. The disclosure also includes all such steps, features, compositions,
and compounds referred to or indicated in this specification, individually or
collectively, and any and all combinations of any or more of such steps or features.
Definitions

[0012] For convenience, before further description of the present disclosure,
certain terms employed in the specification, and examples are delineated here.
These definitions should be read in the light of the remainder of the disclosure and
understood as by a person of skill in the art. The terms used herein have the
meanings recognized and known to those of skill in the art, however, for
convenience and completeness, particular terms and their meanings are set forth
below.
[0013] The articles "a", "an" and "the" are used to refer to one or to more than one
(i.e., to at least one) of the grammatical object of the article.
[0014] The terms "comprise" and "comprising" are used in the inclusive, open
sense, meaning that additional elements may be included. It is not intended to be
construed as "consists of only".
[0015] Throughout this specification, unless the context requires otherwise the
word "comprise", and variations such as "comprises" and "comprising", will be
understood to imply the inclusion of a stated element or step or group of element or
steps but not the exclusion of any other element or step or group of element or
steps.
[0016] The term "including" is used to mean "including but not limited to".
"Including" and "including but not limited to" are used interchangeably.
[0017] Ratios, concentrations, amounts, and other numerical data may be presented
herein in a range format. It is to be understood that such range format is used
merely for convenience and brevity and should be interpreted flexibly to include
not only the numerical values explicitly recited as the limits of the range, but also
to include all the individual numerical values or sub-ranges encompassed within
that range as if each numerical value and sub-range is explicitly recited. For
example, a temperature ranges of about 22-28 °C should be interpreted to include
not only the explicitly recited limits of about 22 °C to about 28 °C, but also to
include sub-ranges, such as 22-25 °C, 25-28 °C, and so forth, as well as individual
amounts, including fractional amounts, within the specified ranges, such as 22.2
°C, and 27.5 °C, for example.

[0018] The term "at least one" is used to mean one or more and thus includes individual components as well as mixtures/combinations.
[0019] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference. [0020] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods are clearly within the scope of the disclosure, as described herein. [0021] As mentioned previously, the existing food supplements suffer from drawbacks of toxicity and high cost. Therefore, there is a scope for exploration of synergistic actives combinations which can provide the better efficacy of health promoting benefits such as enhanced iron uptake. Herbal or natural combinations could prove effective in providing enhanced iron-uptake while being non-toxic. The present disclosure aims to solve the said problem by employing a combination consisting of ginger extract and an organic compound shows the synergistic effect on the iron absorption.
[0022] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1. In another embodiment of the present disclosure, the Zingiber officinale extract to the at least one organic compound weight ratio is in arange of 0.3:1 to 4.8:1.
[0023] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.5:1 to 4.5:1. In another embodiment of the

present disclosure, the Zingiber officinale extract to the at least one organic
compound weight ratio is in a range of 1:1 to 4:1.
[0024] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is in a range of 1.5:1 to 2.5:1. In another embodiment of the
present disclosure, the Zingiber officinale extract to the at least one organic
compound weight ratio is in a range of 1.5:1 to 2.2:1.
[0025] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is 0.67:1.
[0026] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is 1.5:1.
[0027] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is 2:1.
[0028] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is 4:1.
[0029] In an embodiment of the present disclosure, there is provided a herbal
composition as described herein, wherein the Zingiber officinale extract comprises
gingerol having a weight percentage in a range of 6 - 8% with respect to the
extract. In another embodiment of the present disclosure, the Zingiber officinale
extract comprises gingerol having a weight percentage in a range of 6 - 7% with
respect to the extract.

[0030] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1 and the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract.
[0031] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.5:1 to 4.5:1 and the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract.
[0032] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 1.5:1 to 2.5:1 and the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract.
[0033] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 1.5:1 and the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract.
[0034] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 2:1 and the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract. [0035] In an embodiment of the present disclosure, there is provided a herbal composition as described herein, wherein the at least one organic compound is

selected from a group consisting of succinic acid, sodium acetate, citric acid,
tartaric acid, malic acid, propionic acid, fumaric acid, and combinations thereof. In
another embodiment of the present disclosure, the at least one organic compound is
succinic acid. In yet another embodiment of the present disclosure, the at least one
organic compound is sodium acetate.
[0036] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is in a range of 0.2:1 to 5:1, and the at least one organic
compound is selected from a group consisting of succinic acid, sodium acetate,
citric acid, tartaric acid, malic acid, propionic acid, fumaric acid, and combinations
thereof.
[0037] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) succinic acid,
wherein Zingiber officinale extract to succinic acid weight ratio is in a range of
0.2:1 to 5:1.
[0038] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) succinic acid,
wherein Zingiber officinale extract to succinic acid weight ratio is 0.67:1.
[0039] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) succinic acid,
wherein Zingiber officinale extract to succinic acid weight ratio is 1.5:1.
[0040] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) succinic acid,
wherein Zingiber officinale extract to succinic acid weight ratio is 2:1.
[0041] embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) succinic acid,
wherein Zingiber officinale extract to succinic acid weight ratio is 4:1.
[0042] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) sodium acetate,

wherein Zingiber officinale extract to sodium acetate weight ratio is in a range of
0.2:1 to 5:1.
[0043] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) sodium acetate,
wherein Zingiber officinale extract to sodium acetate weight ratio is 0.67:1.
[0044] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) sodium acetate,
wherein Zingiber officinale extract to odium acetate weight ratio is 1.5:1.
[0045] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) sodium acetate,
wherein Zingiber officinale extract to sodium acetate weight ratio is 2:1.
[0046] embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) sodium acetate,
wherein Zingiber officinale extract to sodium acetate weight ratio is 4:1.
[0047] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is in a range of 0.2:1 to 5:1, the Zingiber officinale extract
comprises gingerol having a weight percentage in a range of 6 - 8% with respect to
the extract, and the at least one organic compound is selected from a group
consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid,
propionic acid, fumaric acid, and combinations thereof.
[0048] In an embodiment of the present disclosure, there is provided a herbal
composition comprising: a) Zingiber officinale extract; and b) at least one organic
compound, wherein the Zingiber officinale extract to the at least one organic
compound weight ratio is in a range of 0.5:1 to 4.5:1, the Zingiber officinale extract
comprises gingerol having a weight percentage in a range of 6 - 8% with respect to
the extract, and the at least one organic compound is selected from a group
consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid,
propionic acid, fumaric acid, and combinations thereof.

[0049] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 1.5:1 to 2.5:1, the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract, and the at least one organic compound is selected from a group consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid, propionic acid, fumaric acid, and combinations thereof.
[0050] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 1.5:1, the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract, and the at least one organic compound is selected from a group consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid, propionic acid, fumaric acid, and combinations thereof.
[0051] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 2:1, the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract, and the at least one organic compound is selected from a group consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid, propionic acid, fumaric acid, and combinations thereof.
[0052] In an embodiment of the present disclosure, there is provided a herbal composition as described herein, wherein the composition comprising: a) Zingiber officinale extract having a weight percentage in a range of 0.001 - 0.007% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.007% with respect to the composition. In another embodiment of the present disclosure, the Zingiber officinale extract having a weight percentage in a range of 0.0025 - 0.0065% with respect to the

composition and the at least one organic compound having a weight percentage in the range of 0.0015 - 0.0065% with respect to the composition. In yet another embodiment of the present disclosure, the Zingiber officinale extract having a weight percentage in a range of 0.0035 - 0.0061% with respect to the composition and the at least one organic compound having a weight percentage in the range of 0.0025 - 0.005% with respect to the composition. In yet another alternate embodiment of the present disclosure, the Zingiber officinale extract having a weight percentage in a range of 0.004 - 0.0048% with respect to the composition and at least one organic compound having a weight percentage in the range of 0.0028 - 0.0035% with respect to the composition.
[0053] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having a weight percentage in a range of 0.001 - 0.007% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.007% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1. [0054] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.001 - 0.007% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.007% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 1.5:1.
[0055] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.001 - 0.007% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.007% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 2:1.
[0056] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.001 - 0.007% with respect to the composition; and b) at least one

organic compound having a weight percentage in the range of 0.001 - 0.007% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1 and the at least one organic compound is selected from a group consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid, propionic acid, fumaric acid, and combinations thereof.
[0057] In an embodiment of the present disclosure, there is provided a herbal composition as described herein, wherein the composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition. [0058] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1. [0059] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 1.5:1.
[0060] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition, wherein Zingiber officinale extract to the at least one organic compound weight ratio is 0.67:1.
[0061] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having a weight percentage

in a range of 0.0025 - 0.0065% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 2:1.
[0062] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition, wherein Zingiber officinale extract to the at least one organic compound weight ratio is 4:1.
[0063] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having a weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; and b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1 and the at least one organic compound is selected from a group consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid, propionic acid, fumaric acid, and combinations thereof.
[0064] In an embodiment of the present disclosure, there is provided a herbal composition as described herein, wherein the composition further comprises at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof. In another embodiment of the present disclosure, the at least one additive is the other extract selected from a group consisting of Feijoa sellowiana extract, Crataegus pentagyna extract, Melilotus arvensis extract, and other known flavonoid containing plant extracts, and combinations thereof.
[0065] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; b) at least one organic compound; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein the Zingiber

officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1. In another embodiment of the present disclosure, the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.3:1 to 4.8:1.
[0066] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract; b) at least one organic compound; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.5:1 to 4.5:1. In another embodiment of the present disclosure, Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 1:1 to 4:1. [0067] In an embodiment of the present invention, there is provided a herbal composition comprising: a) Zingiber officinale extract; b) at least one organic compound; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 1.5:1 to 2.5:1. In another embodiment of the present disclosure, the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 1.5:1 to 2.2:1.
[0068] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1.
[0069] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with

respect to the composition; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 1.5:1.
[0070] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 0.67:1.
[0071] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is 4:1.
[0072] In an embodiment of the present disclosure, there is provided a herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition; and c) at least one additive selected from a group consisting of carriers, chelating agent, other extract, and combinations thereof, wherein Zingiber officinale extract to the at least one organic compound weight ratio is 2:1.
[0073] In an embodiment of the present disclosure, there is provided a process for preparing the herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at

least one organic compound weight ratio is in a range of 0.2:1 to 5:1, said process comprising steps of: a) obtaining Zingiber officinale extract; b) at least one organic compound; and c) contacting Zingiber officinale extract with at least one organic compound to obtain the composition.
[0074] In an embodiment of the present disclosure, there is provided a process for preparing the herbal composition comprising: a) Zingiber officinale extract having weight percentage in a range of 0.001 - 0.007% with respect to the composition; and b) at least one organic compound having a weight percentage in a range of 0.001 - 0.007% with respect to the composition, wherein Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1, said process comprising steps of: a) obtaining Zingiber officinale extract; b) at least one organic compound; and c) contacting Zingiber officinale extract with at least one organic compound to obtain the composition.
[0075] In an embodiment of the present disclosure, there is provided a process for preparing the herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.5:1 to 4.5:1, said process comprising steps of: a) obtaining Zingiber officinale extract; b) at least one organic compound; and c) contacting Zingiber officinale extract with at least one organic compound to obtain the composition.
[0076] In an embodiment of the present disclosure, there is provided a process for preparing the herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 1.5:1 to 2.5:1, said process comprising steps of: a) obtaining Zingiber officinale extract; b) at least one organic compound; and c) contacting Zingiber officinale extract with at least one organic compound to obtain the composition.
[0077] In an embodiment of the present disclosure, there is provided a process for preparing the herbal composition comprising: a) Zingiber officinale extract; and b) at least one organic compound, wherein Zingiber officinale extract to the at least one organic compound weight ratio is 1.5:1, said process comprising steps of: a)

obtaining Zingiber officinale extract; b) at least one organic compound; and c)
contacting Zingiber officinale extract with at least one organic compound to obtain
the composition.
[0078] In an embodiment of the present disclosure, there is provided a process for
preparing the herbal composition comprising: a) Zingiber officinale extract; and b)
at least one organic compound, wherein the Zingiber officinale extract to the at
least one organic compound weight ratio is 2:1, said process comprising steps of: a)
obtaining Zingiber officinale extract; b) at least one organic compound; and c)
contacting Zingiber officinale extract with at least one organic compound to obtain
the composition.
[0079] In an embodiment of the present disclosure, there is provided a process for
preparing the herbal composition comprising: a) Zingiber officinale extract; and b)
at least one organic compound, wherein Zingiber officinale extract to the at least
one organic compound weight ratio is 0.67:1, said process comprising steps of: a)
obtaining Zingiber officinale extract; b) at least one organic compound; and c)
contacting Zingiber officinale extract with at least one organic compound to obtain
the composition.
[0080] In an embodiment of the present disclosure, there is provided a process for
preparing the herbal composition comprising: a) Zingiber officinale extract; and b)
at least one organic compound, wherein Zingiber officinale extract to the at least
one organic compound weight ratio is 4:1, said process comprising steps of: a)
obtaining Zingiber officinale extract; b) at least one organic compound; and c)
contacting Zingiber officinale extract with at least one organic compound to obtain
the composition.
[0081] In an embodiment of the present disclosure, there is provided a herbal
composition as described herein, wherein the composition provides enhanced iron
uptake.
[0082] In an embodiment of the present disclosure, there is provided a herbal
composition as described herein, wherein the composition may be dispensed as part
of commercial formulations such as health supplements. In another embodiment of
the present disclosure, the composition may be dispensed as a biscuit or as part of a

beverage. Other well-known commercial forms may be considered for dispensing the composition. EXAMPLES
[0083] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may apply. [0084] As described above, there is a scope for exploration of synergistic actives combinations which can provide the better efficacy of health promoting benefits. The present disclosure reveals a combination comprising a) Zingiber officinale extract; and b) at least one organic compound, wherein Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1. [0085] Source of actives: Zingiber officinale or ginger extract was obtained from Arjuna natural extracts. Succinic acid, sodium acetate and sodium ascorbic acid -Vitamin C were obtained commercially from Sigma. Example 1: Identification of active for iron absorption
[0086] Iron uptake assay was studied on Caco2 cells (P47-P52). Caco2 cells were seeded on a 24 well plate at density 5X104 cells/ well. Post confluency of 14 days, cells were used for iron uptake assay. Caco2 cells were washed with iron free HBSS (Hanks Balanced Salt Solutions from thermo fisher scientific) then cells were treated with the actives and its combinations in the presence of ferrous sulphate (lOuM) for 60 min at 5% C02, 37°C. After incubation, media was removed and
cells were washed twice with ice cold HBSS. Samples were lysed with 50mm NaOH and followed by sonication at 25%amp for 5s. Then some quantity (50ul)

used for protein estimation by BCA method and remaining used for total
intracellular iron content by using ferrozine method.
[0087] Estimation of total iron by ferrozine method: Aliquots of tested lysate
samples (lOOul) were mixed with equal quantity of lOmM of HC1 and freshly
prepared iron releasing reagent (1.4MHC1 and 4.5%KMn04 in 1:1 ratio) and
incubated for 2h at 60°C. Post incubation, samples were cooled to room
temperature and mixed with 30ul of iron detection reagent (freshly mixed 6.5mM
ferrozine, 2.5m ammonium acetate and 1M of Ascorbic acid) and incubated for
30min, then absorbance was measured at 550nm on a microplate reader. Percentage
of iron absorption was calculated for each tested samples.
[0088] Among the all combinations, Zingiber officinale or ginger extract
(0.005%) and succinic acid (0.0025%) combination (Figures 1 and 2) showed the
high iron absorption ability (-31%) as compared to control (FeS04). The
combination pertaining to Zingiber officinale or ginger extract (0.005%) and
sodium acetate (0.0025%) was also found to show synergistic enhancement
(19.53% in Figure 1).
Example 2: Evaluation of selected combination at different ratios for iron
absorption ability
[0089] The process as described in Example 1 was followed in order to test
various ratios of a combination comprising: a) Zingiber officinale or ginger extract;
and b) at least one organic compound for iron uptake.
[0090] Among the all ratios tested for iron absorption (Figure 3), Zingiber
officinale or ginger extract and succinic acid combination at 1.5:1 ratio showed
synergistic iron absorption rate (-52%). The other combinations pertaining to 2:1,
4:1, 2:3 or 0.67:1 and 1:4 or 0.25:1 were also found to enhance iron uptake. Thus,
the combination (ginger extract and succinic acid) proved to have synergistic iron
absorption ability as compared to individual.
Advantages of the present disclosure:
[0091] The present disclosure reveals a herbal composition comprising Zingiber
officinale or ginger extract; and b) at least one organic compound. The composition

was found to enhance cellular iron uptake. The composition can be easily adapted to obtain useful commercial formulations such as health supplements.

I/We Claim;
1. A herbal composition comprising:
a) Zingiber officinale extract; and
b) at least one organic compound,
wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.2:1 to 5:1.
2. The herbal composition as claimed in claim 1, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 0.5:1 to 1:4.5.
3. The herbal composition as claimed in claim 1, wherein the Zingiber officinale extract to the at least one organic compound weight ratio is in a range of 1.5:1 to 2.5:1.
4. The herbal composition as claimed in claim 1, wherein the Zingiber officinale extract comprises gingerol having a weight percentage in a range of 6 - 8% with respect to the extract.
5. The herbal composition as claimed in claim 1, wherein the at least one organic compound is selected from a group consisting of succinic acid, sodium acetate, citric acid, tartaric acid, malic acid, propionic acid, fumaric acid, and combinations thereof.
6. The herbal composition as claimed in any one of the claims 1-5, wherein the composition comprises:

a) Zingiber officinale extract having weight percentage in a range of 0.001 - 0.007% with respect to the composition; and
b) at least one organic compound having a weight percentage in a range of 0.001 - 0.007% with respect to the composition.
7. The herbal composition as claimed in claim 6, wherein the composition
comprises:
a) Zingiber officinale extract having a weight percentage in a range of 0.0025 - 0.0065% with respect to the composition; and
b) at least one organic compound having a weight percentage in the range of 0.001 - 0.005% with respect to the composition.

8. The herbal composition as claimed in any one of the claims 1-7, wherein the composition further comprises at least one additive selected from a group consisting of carriers, chelating agent, and combinations thereof.
9. A process for preparing the herbal composition as claimed in claim 1, said process comprising the steps of: a) obtaining Zingiber officinale extract; b) obtaining at least one organic compound; and c) contacting the Zingiber officinale extract with the at least one organic compound to obtain the composition.
10. The herbal composition as claimed in any one of the claims 1-8, wherein the composition provides enhanced iron uptake.