The present invention relates to a method for systemic adjuvant for improving the nutrient use efficiency for living beings. The present invention in particular relates to the synthesis of systemic adjuvant comprising of encapsulation of bioavailable Silicon with natural peptide sources along with other key ingredients, derived from natural eco-friendly organic sources. Different formulations of adjuvants have been developed for applications in different life forms using peptides from varied natural sources. -
DESCRIPTION OF THE RELATED ART:
Agricultural fertilizers are well-known in the art. Additionally, foliar growth promoting mixtures have also been known in the art. The closest agricultural products known to the Applicant includes U.S. Pat. Nos. 3,377,152; 43,639; 2,158,918; 4,043,788; 1,550,268; 3,940,257; 99,152; 2,992,093; 2,129,334; 3,630,710; 2,317,991; 3,674,649; 2,856,286; and, 4,126,439.
The closest reference in the opinion of Applicant is U.S. Pat. No. 4,043,788. However, in this reference, it is believed that the major proportion of fertilizer is formed of the fish hydrolysate as the key component. When a high amount of fish hydrolysate is used, there is a supply to the plant of more of the major nutrients, however, there is obviously less of an input of the trace elements which is found in the major proportion of the subject invention concept where liquid seaweed is being used. The additional liquid seaweed regulates and controls the enzymatic processes and aids during times of plant stress. By using the composition shown in U.S. Pat. No. 4,043,788, there would be a minimal amount of growth during a stress period and as is known, when one counts on the major nutrients, there is a high primary growth, but such is at a very low rate. The use of additional liquid seaweed is believed to act as a catalyst, thereby increasing the amount of Nitrogen, Potassium, and Phosphorus, which is absorbed by the plant through either the soil or the leaf and stem portion. The formulation is aimed for foliar application as a standalone product imparting various growth promoting benefits to crop and culminating to higher yields.
Reference may be made to the following:
Publication No. US4383845 relates to foliar growth promoting mixture for increasing crop yield as well as protein content within plants. The foliar growth promoting mixture includes a liquid seaweed base composition which forms approximately 50.0% by weight of the foliar growth promoting mixture. To the liquid seaweed base composition is blended a fish emulsion mixture k^hich forms

approximately 25.0% by weight of the overall foliar growth promoting mixture. A liquid humus composition within the approximate weight percentage of between 5.0%-15.0% of the overall mixture is included and a water soluble nutrient from the group consisting of carbohydrates and enzymes within the approximate weight range of 10.0%-20.0% of the foliar growth promoting mixture is further added.
Publication No. 20130130902 relates to release of bioavailable silicon from silicate material insitu and thereafter maintaining them in bioavailable form using osmolytes and other ingredients of the formulations.
Publication No. US20160200634 relates to a bio-available mineral fertilizer composition including highly pulverized powdered mixture of silicate and other bio minerals fortified with seaweed extracts, other plant nutrients, humic and fuvlic acid fractions along with a consortium of beneficial microorganisms (bacteria, fungi, actinomycetes, VAM and bio fertilizers). The formulation has multiple uses as fertilizers for soil and foliar sprays.
Reference may be made to the article entitled "Bioavailability of Silicon, A Technological Challenge" by Iontec talks about technology which makes it possible to stabilise silicon in a powder form so that it does not polymerise. By trapping the silicon in a collagen peptide matrix, bonds were created between the amines and the hydroxyl groups of amino acids and orthosilicic acid. Once it is ingested, this peptide matrix is broken down in the stomach into amino acids, liberating the silicon from which it was created. Iontec's technology is based on the use of find micronized powders of silicate minerals conjugated with naturals chelating agents and other nutrients ensuring optimal delivery of supplied nutrients following cellular metabolism.
Accordingly, there is an urgent need of a source of bioavailable silicon which is compatible with conventional nutrient mixtures for different life forms. Generally, nutrients are supplied in high quantities out of which only a fraction is utilized by living organism. The excess of supplied nutrients generally remains unutilized thus creates imbalance in ecosystems.
The present invention aims to provide a method for preparing silica collagen peptide powder for natural fertilizer. The invention provides a water soluble powdered formulation, a systemic adjuvant aimed to use along with conventionally used nutrient sources for different life forms.

Addition of adjuvant ensures sustainable release of nutrients from the bulk sources supplied along with. The regulated release of nutrients optimizes their assimilation and translocation efficiency.
OBJECTS OF THE INVENTION:
The primary objective of the present invention is to provide a source of bioavailable silicon which is compatible with conventional nutrient mixtures for different life forms. The addition of bioavailable silicon would improve the use efficiency of these nutrients to maximize the benefits of supplied nutrients to the used.
Another object of the present invention is to optimize the nutrients requirement, by improving their use efficiency.
Yet another object of the present invention is to expand the area of application of the bioavailable silicon to other life forms.
Still another object of the present invention is to provide silica collagen peptide powder and Silica- Fish hydrolyzate powder as natural fertilizer.
Another object of the present invention is to provide a method for preparing silica collagen peptide powder for natural fertilizer.
Yet another object of the present invention is to provide a method of synthesis of stabilized water soluble complex of silica-collagen peptide powder.
Still another object of the present invention is to provide a method of synthesis of Silica- Fish hydrolyzate powder to be used as natural fertilizer.
SUMMARY OF THE INVENTION:
The present invention provides a method for encapsulation of bioavailable silicon using Bovine Collagen peptide powder or Fish hydrolyzate powder for its application in such as but not limited to animal husbandry, agricultural products and nutraceutical industries. The key ingredients used in the encapsulation technology ensure rapid translocation and assimilation of nutrients in various life forms. The encapsulation ensures the stabilization of molecular clusters of silicic acid for its

optimal efficacy for longer duration of time. The use of two different sources of peptides are standardize during the invention ascertains wider application of bioavailable silicon.
The adjuvant of encapsulation of bioavailable silicon aimed for agriculture is done using the fish hydrolyzate proteins targeted to be mixed with bulk and composite fertilizers applied through soil. Apart from this a fully water soluble formulation of encapsulation of bioavailable silicon is made using bovine collagen peptides. The use of the invention is not restricted to plants only. The systemic adjuvant developed forms an acidic solution (1%) and is completely water soluble. The fish hydrolyzate powder ensures the retention of molecular clusters of silicic acid, thereby preventing the formation of oligomers/polymers, thus ensuring efficacy of the formulation for a longer durations. Other key ingredients used in the adjuvant facilitate sustainable release of nutrients from the bulk sources of fertilizers used along, for optimum translocation and assimilation. The adjuvant has compatibility with wider range of products thus ensuring its wider applications.
The present invention also provides a novel method of formulation of natural adjuvant for fertilizers, having systemic role, aimed to be used for agricultural purpose. In this method the molecular clusters of silicic acid are stabilized using fish hydrolyzate powder fortified with natural polysaccharides (viz. Maltodextrin, Starch, Glycogen, Chitin, Pectin, Cellulose etc.), fatty acid and potassium salt of organic acids viz. Potassium Lactate, Potassium Gluconate, potassium Tartarate, Potassium Citrate etc. The formulation is aimed to be used as a systemic adjuvant when mixed with the conventional nutrients supplied to plants, animals husbandry and nutraceuticals. Presence of adjuvant along with varied nutrient optimizes their use efficiency by facilitating the translocation and assimilation.
The present invention aimed at the synthesis of stabilized water soluble complex of silica-collagen peptide powder. The silicon is encapsulated in micronized polymer of bovine collagen peptide and Methyl Sulfonyl Methane and potassium salt of organic acids (viz. Potassium Lactate, Potassium Gluconate, Potassium Citrate etc.), which makes it stable and water soluble powdered form. The key ingredient i.e. Potassium silicate is reacted with strong acid like HCL , H3P04 , H2S04 to form Ortho silicic acid and The other ingredients i.e. stabilizers, methyl sulfonyl methane, organic acids, polysaccharides, Potassium Gluconate and peptide sources are used to cross link with monomeric ortho silicic acid to facilitate their retention in the monomeric forms according to the application. This process is done at temperature below 25degreeC. The reaction

is done in a High speed reactor (rpm 700-1000). The homogenous liquid formulation as soon as it is prepared is immediately spray dried under low pressure to get the powdered product.
BREIF DESCRIPTION OF THE DRAWINGS:
It is to be noted, however, that the appended drawings illustrate only typical embodiments of this invention and are therefore not to be considered for limiting of its scope, for the invention may admit to other equally effective embodiments.
Figure 1 shows percentage increase in the content of major nutrients in rice when bio available
silicon technical was mixed with NPK.
Figure 2 shows yield comparison (t/ha) in sugarcane when cultivated with two levels of
recommended dose of fertilizers with and without fortification of bio available silicon.
Figure 3 shows impact of OS A when fed to 1-day-old chickens on the serum calcium levels and
biomechanical strength of femur bone.
Figure 4 shows impact of OSA on the oxidative stress on in vitro culture of mammalian cells (a)
Cell viability (%) (b) Levels of LDH (% secretion)
Figure 5 shows impact of OSA supplementation on the bone health in humans (a) Prote(in neo
synthesis in bone matrix (b) Calcium anabolism in bone
DETAILED DESCRIPTION OF THE INVENTION:
The present invention provides a method for encapsulation of bioavailable silicon using Bovine Collagen peptide powder or Fish hydrolyzate powder for its application in such as but not limited to animal husbandry, agricultural products and nutraceutical industries.
The present invention also provides a novel method of formulation of natural adjuvant for fertilizers, having systemic role, aimed to be used for agricultural purpose. In this method the molecular clusters of silicic acid are stabilized using fish hydrolyzate powder fortified with natural polysaccharides (viz. Maltodextrin, Starch, Glycogen, Chitin, Pectin, Cellulose etc.), fatty acid and potassium salt of organic acids viz. Potassium Lactate, Potassium Gluconate, potassium Tartarate, Potassium Citrate etc. The formulation is aimed to be used as a systemic adjuvant when mixed with the conventional nutrients supplied to plants, animals husbandry and nutraceuticals. Presence of adjuvant along with varied nutrient optimizes their use efficiency by facilitating the translocation and assimilation.

The present invention aimed at the synthesis of stabilized water soluble complex of silica-collagen peptide powder. The silicon is encapsulated in micronized polymer of bovine collagen peptide and Methyl SulfonylMethane and potassium salt of organic acids (viz. Potassium Lactate, Potassium Gluconate, Potassium Citrate etc.), which makes it stable and water soluble powdered form. The key ingredient i.e. Potassium silicate is reacted with strong acid like HCL , H3P04 , H2S04 to form Ortho silicic acid and The other ingredients i.e. stabilizers, methyl sulfonyl methane, organic acids, polysaccharides, Potassium Gluconate and peptide sources are ised to cross link with monomeric ortho silicic acid to facilitate their retention in the monomeric forms according to the application. This process is done at temperature below 25degreeC. The reaction is done in a High speed reactor (rpm 700-1000). The homogenous liquid formulation as soon as it is prepared is immediately spray dried under low pressure to get the powdered product.
The use of the invention is not restricted to plants only. The systemic adjuvant developed forms an acidic solution (1%) and is completely water soluble. The fish hydrolyzate powder ensu-es the retention of molecular clusters of silicic acid, thereby preventing the formation of oligomers/polymers, thus ensuring efficacy of the formulation for a longer durations. Other key ingredients used in the adjuvant facilitate sustainable release of nutrients from the bulk sources of fertilizers used along, for optimum translocation and assimilation. The adjuvant has compatibility with wider range of products thus ensuring its wider applications.
The present invention is described by reference to the following Examples, which are offered by way of illustration and are not intended to limit the invention in any manner. Standard techniques well known in the art or the techniques specifically described below were utilized. EXAMPLE 1
Method for preparation of formulation
For the preparation of formulation of natural adjuvant for fertilizers, having systemic role, aimed to be used for agricultural purpose, the molecular clusters of silicic acid were stabilized using fish hydrolyzate powder fortified with natural polysaccharides (viz.. Maltodextrin, Starch, Glycogen, Chitin, Pectin, Cellulose etc.), fatty acid and potassium salt of organic acids viz. Potassium Lactate, Potassium Gluconate, potassium Tartarate, Potassium Citrate etc. the formulation is aimed to be used as a systemic adjuvant when mixed with the conventional nutrients supplied to plants, animals husbandry and nutraceuticals. Presence of adjuvant along with varied nutrient optimizes their use efficiency by facilitating the translocation and assimilation.

EXAMPLE 2
Composition of formulation
Silicon (Si) 0.01-4%
Bovine Collagen Peptide 10-70%
Methyl Sulfonyl Methane 5-30%
and/or
Potassium salt of Organic Acids : 1 -10%
EXAMPLE 3 Composition of formulation
Silicon(Si) . : 0.01-4%
Bovine Collagen Peptide : 10-80% Methyl Sulfonyl Methane : 5-15% and /or Potassium Salt of Organic Acids : 2-8%
EXAMPLE 4 Composition of formulation
Silicon(Si) : 0.01-5%
Fish hydrolyzate : 10-40%
Natural Polysaccharides : 2-20%
and/or Potassium salt of Organic Acids : 2-10%
EXAMPLE 5 Composition of formulation
Silicon (Si) : 0.01-5%
Fish hydrolyzate : 05-35%
Natural Polysaccharides : 1-15%
and/or Potassium Salt of Organic Acids : 2-8%

An analysis was conducted where water soluble powder of bioavailable silicon @ 2 kg to 5kg per 1000 kg, was physically mixed with recommended dose of fertilizers prior to its application. The nutrient profiling was done at critical stages of crop to ascertain the nutritional status (Figure 1). The addition of bioavailable silicon to NPK fertilizer has improved the nutritional status of leaves for all major nutrients, with a promising impact on availability of phosphorous fertilizers. Study was further continued to confirm if the fortification of bioavailable silicon can lead to a reduction in dose of fertilizer without yield compromise. Sugarcane, known heavy feeder of fertilizers, was selected as the experimental system. It was cultivated with two leyels of recommended dose of fertilizers viz. 100% and 75% with and without fortification of bioavailable silicon (2 kg in 1000 kg).
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The yields were compared at harvest. Thus fortification of bioavailable silicon can reduce the fertilizer dose upto 25% without any yield compromise (Figure 2).
EXAMPLE 6: Impact of bioavailable silicon powder on the health of chicken.
One day old chicken with average body weight of 40 g were taken for analysis. During! entire
duration of six weeks of study, they were fed with diet supplemented with 5% bioavailable
silicon. More than 7.0% increases in the calcium levels of serum were recorded (Figure 3 a).
Increased levels of calcium in serum are reflective of stronger bones. This was supported |by the
i biomechanical strength of femur, thigh bone (Figure 3b).
EXAMPLE 7: Impact of bioavailable silicon on the bone health and distressing in humans.;
Silicon has a documented role as a stress reliever. For this mammalian cells were cultured in vitro
i
with varied levels of OSA supplemented medium. Cell viability and levels of Lactate dehydrogenase (LDH), an indicator of cell damage were recorded. OSA supplemented medium has exhibited an increase in call viability in presence of hydrogen peroxide with lower levels of LDH, indicating the role of OSA in delaying apoptosis (Figure 4 a, b).
Impact of OSA was recorded for improved bone health by estimating the levels of new protein levels in bone matrix as well as by levels of calcium incorporation and retention (Figure 5 a,b). Observations of above described preliminary studies are indicative of beneficial impact, of water soluble powdered formulation of bioavailable silicon with collagen peptide and fish hydrolysate powder for wide applications ranging from plants, animals and humans.

Numerous modifications and adaptations of the system of the present invention will be apparent to those skilled in the art, and thus it is intended by the appended claims to cover all such modifications and adaptations which fall within the true spirit and scope of this invention.

WE CLAIM:

1.A method for water soluble powdered formulation of bioavailable Silicon by stabilizing molecular clusters of silicic acid using fish hydrolyzate powder fortified with natural polysaccharides, fatty acid and potassium salt of organic acids or preparing complex of silica-collagen peptide powder.
2. The method for water soluble powdered formulation of bioavailable Silicon as claimed in claim 1 wherein polysaccharides are selected from Maltodextrin, Starch, Glycogen, Chitin, Pectin, Cellulose and potassium salt of organic acids are selected from Potassium Lactate, Potassium Gluconate, potassium Tartarate, Potassium Citrate.
3. The method for water soluble powdered formulation of bioavailable Silicon as claimed in claim 1 wherein for the synthesis of stabilized water soluble complex of silica-collagen peptide powder, silicon is encapsulated in micronized polymer of bovine collagen peptide and Methyl Sulfonyl Methane and potassium salt of organic acids.
4. The method for water soluble powdered formulation of bioavailable Silicon as claimed in claim 1 wherein the silicon is encapsulated in micronized polymer of bovine collagen peptide and Methyl Sulfonyl Methane and potassium salt of organic acids, which makes it stable and water soluble powdered form, the key ingredient i.e. Potassium silicate is reacted with strong acid like HCL , H3P04 , H2S04 to form Ortho silicic acid and the other ingredients i.e. stabilizers, methyl sulfonyl methane, organic acids, polysaccharides, Potassium Gluconate and peptide sources are used to cross link with monomeric ortho silicic acid to facilitate their retention in the monomeric forms is done at temperature below 25degreeC, a high speed reactor (rpm 700-1000) and homogenous liquid formulation is immediately spray dried under low pressure to get the powdered product.
5. The method for water soluble powdered formulation of bioavailable Silicon as claimed in claim 1 wherein the formulation comprises of Silicon (Si) 4%, Bovine Collagen Peptide 50%, Methyl Sulfonyl Methane 30%, and Potassium salt of Organic Acids 10%.
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