A Scalable Method For Producing Retinal Pigment Epithelium (Rpe) Cells

Abstract: ABSTRACT A SCALABLE METHOD FOR PRODUCING RETINAL PIGMENT EPITHELIUM (RPE) CELLS The present disclosure provides a method for obtaining RPE (retinal pigment epithelium) cells from iPSCs (induced pluripotent stem cells) comprising: (a) generating embryoid bodies from a culture of iPSCs, wherein the embryoid bodies are in non-adherent suspension culture, (b) plating the embryoid bodies on a culture dish coated with a suitable extracellular matrix in differentiation induction media (DIM), wherein the DIM comprises at least one WNT pathway inhibitor and at least two SMAD pathway inhibitors, (c) culturing the neuroectoderm lineage in differentiation propagation media (DPM) for rosette formation, (d) culturing the rosettes of step (c) in Retinal Pigment Epithelium Maturation Media (RPEMM) to facilitate retinal progenitor cells formation, and (e) plating the retinal progenitor cells of step (d) on a culture dish coated with suitable extracellular matrix in RPEMM to obtain RPE cells.

Patent Information

Application #

Filing Date

14 October 2022

Publication Number

16/2024

Publication Type

INA

Invention Field

BIOTECHNOLOGY

Status

Email

Parent Application

Applicants

EYESTEM RESEARCH PRIVATE LIMITED

GROUND FLOOR, CENTRE FOR CELLULAR AND MOLECULAR PLATFORMS (C-CAMP), GKVK POST, BENGALURU KARNATAKA 560065, INDIA

Inventors

1. PAL, Rajarshi

G135, Fortuna Center Park, Canara Bank Layout, Kodigehalli, Bengaluru Karnataka 560065, India

2. SURENDRAN, Harshini

P4, Patteshwara Park Apartments, Ramaswamy Layout, Velandipalayam, Coimbatore Tamilnadu 641025, India

3. LALITHA SOUNDARARAJAN

17C, Mary Martin Sadan, 8th Cross, Pampa Extension, Hebbal Kempapura Bangalore Karnataka 560024, India

4. SUBRAMANI, Janavi

No. 24, 6th cross, Saraswathipuram, Nandini Layout, Bangalore Karnataka 560096, India

Specification

DESC:A SCALABLE METHOD FOR PRODUCING RETINAL PIGMENT EPITHELIUM (RPE) CELLS ,CLAIMS:AS ATTACHED

Documents

Application Documents

#	Name	Date
1	202241058893-STATEMENT OF UNDERTAKING (FORM 3) [14-10-2022(online)].pdf	2022-10-14
2	202241058893-PROVISIONAL SPECIFICATION [14-10-2022(online)].pdf	2022-10-14
3	202241058893-FORM FOR STARTUP [14-10-2022(online)].pdf	2022-10-14
4	202241058893-FORM FOR SMALL ENTITY(FORM-28) [14-10-2022(online)].pdf	2022-10-14
5	202241058893-FORM 1 [14-10-2022(online)].pdf	2022-10-14
6	202241058893-EVIDENCE FOR REGISTRATION UNDER SSI(FORM-28) [14-10-2022(online)].pdf	2022-10-14
7	202241058893-DRAWINGS [14-10-2022(online)].pdf	2022-10-14
8	202241058893-Proof of Right [31-10-2022(online)].pdf	2022-10-31
9	202241058893-FORM-26 [31-10-2022(online)].pdf	2022-10-31
10	202241058893-FORM 13 [13-10-2023(online)].pdf	2023-10-13
11	202241058893-DRAWING [13-10-2023(online)].pdf	2023-10-13
12	202241058893-CORRESPONDENCE-OTHERS [13-10-2023(online)].pdf	2023-10-13
13	202241058893-COMPLETE SPECIFICATION [13-10-2023(online)].pdf	2023-10-13
14	202241058893-AMENDED DOCUMENTS [13-10-2023(online)].pdf	2023-10-13
15	202241058893-Request Letter-Correspondence [02-11-2023(online)].pdf	2023-11-02
16	202241058893-Power of Attorney [02-11-2023(online)].pdf	2023-11-02
17	202241058893-FORM28 [02-11-2023(online)].pdf	2023-11-02
18	202241058893-Form 1 (Submitted on date of filing) [02-11-2023(online)].pdf	2023-11-02
19	202241058893-Covering Letter [02-11-2023(online)].pdf	2023-11-02
20	202241058893-CERTIFIED COPIES TRANSMISSION TO IB [02-11-2023(online)].pdf	2023-11-02
21	202241058893-FORM 3 [06-03-2024(online)].pdf	2024-03-06
22	202241058893-RELEVANT DOCUMENTS [05-12-2024(online)].pdf	2024-12-05
23	202241058893-FORM 13 [05-12-2024(online)].pdf	2024-12-05