Description
Circumferential hollow hammering chisel and mallet ( CHH chisel & Mallet)
Introduction
Stem cells are clonogenic cells which is capable of differentiation into any type of cell with varying degree potency and plasticity. Adult stem cells have been isolated from a variety of sources which includes bone marrow, brain, skin, hair follicles, skeletal muscle and especially dental tissues.1 Dental stem cells are considered to be an appealing source for mesenchymal stem cells, since they are non-controversial, readily accessible, have a large donor pool, and pose no risk of discomfort for the donor.2
The presence of stem cells have been identified in various dental tissues such as in the pulp of the Primary(SHEDs)3 and Permanent teeth(DPSCs)4, Periodontal Ligament(PDLSCs)5, Periapical Follicle(DFSCs)6 and Apical Papillae(SCAPs)7.
Efistory
Retrieval of pulp tissue stands as a greater challenge in laboratory as they lack dental set up. Studies have quoted about cutting the tooth using a diamond disc. However Pulp retrieval using a diamond disc was not employed as it can cause heat and severe mechanical stress to the pulpal tissues.8
Simple breaking down of the tooth wrapped inside a sterile glove with a chisel and mallet can cause the tooth to slip and also provide a unsupported force. Other method employed in the literature was by access opening the tooth and retrieval by barbed broach, but this method also causes mechanical stress and damages the normal anatomy of the pulp. Further it is again difficult to remove the pulpal tissues from the broach.

Disadvantages of traditional methods
Physical and mechanical damage to pulpal tissues and overheating of pulp.Objective and advantages of circumferential hollow hammering chisel and mallet methodStem cell retrieval can ideal and more efficien using a circumferential hollow hammering chisel and mallet for isolation and characterization of stem cells With the use of CHH chisel and mallet the tooth which is placed inside a sterile glove is totally covered inside the circumfrential metal cup, it doesn't slip off and a steady supported force can be delivered to the tooth which is centered from within.
Parts of CHH chisel and mallet
Chisel-C
Mallet - M
Hollow cup - O
Dimensions of CHH chisel and mallet
Chisel - C
Thickness - 2mm to 10 mm
width - 5mm to 10 mm
Height - 5mm to 30mm
Shape - circumferential
Parts of Mallet - M
Flat surface - F
Head-H
Body-B

Plat surface - F Thickness - 2mm to 5 mm width - 5mm to 10 mm Height - 5mm to ,15mm Shape - round and conical
Head-H
Thickness - 2mm to 5 mm width - 5mm to 10 mm Height - 5mm to 20mm Shape - round and conical
Body-B
Thickness - 2mm to 5 mm width - 5mm to 10 mm Height - 5mm to 25mm Shape - cylindrical
Hollow cup - O Thickness - 2mm to 5 mm width - 5mm to 15 mm Height - 5mm to 25mm Shape - cylindrical

Experimental support
The samples collected from each individual were separately transferred to 15ml conical base centrifuge tubes (BD Falcon) containing Dulbecco's Phosphate Buffer Saline (DPBS) solution (Invitrogen) as a transport medium and taken to the laboratory. Employing the CHH chisel and mallet method the success rate of tooth positive for stem cell is 77% the result obtained is listed below:
Conclusion
So it has been proved that CttH ciiisfei and mallet method is the most reliable method for isolation of stem cells from the tooth_where pulp can be retrieved as a whole without any degree of trauma to the pulpal tissues arid facilitate further research where successful isolation and characterization of stem cells is guaranteed.

I claim
1. A novel Circumferential hollow hammering chisel and mallet comprising of chisel part, mallet parf-and a hollow part with specific thiickness, height, width and shape.
2. A novel Circumferential hollow hammering chisel and mallet
joys pediatric syringe as claimed in claim 1 wherein-the mallet part has got flat surfacejhead and body portions.
3. A novel Circumferential hollow hammering chisel and mallet as claimed in claim 1 where in the Circumferential hollow hammering chisel and mallet is made of any type of metal.
4. A novel Circumferential hollow hammering chisel and mallet substantially as herein defined and illustrated in the figures of the accompanying drawings.