DESC:FIELD OF THE INVENTION
The present invention relates to a composition containing omega-3 enriched Egg powder and other functional constituents derived from plants. More specifically, said omega-3 enriched egg powder is derived from Nutrient-Enhanced (NE) egg obtained from birds that are fed with fish oil and its derivatives along with their regular feed. Said composition is suitably used for foods, supplements, feedstuffs and pharmaceutical and the likes. The invention further discloses a method of manufacturing said composition.
BACKGROUND OF THE INVENTION
There is demand for Omega-3 rich supplements and food products, especially EPA and DHA rich supplement for kids, adults, and animals. About 250-500mg per day of EPA and DHA is recommended for adult human, but then again if it is for patients with a cardiovascular condition the dosage might go up. The benefit of omega-3 and other vital nutrients are not limited to just humans, there are many conditions in animals that can be prevented, and/or cured by supplementing omega-3 in an effective dose to animals. There is a challenge when it comes to supplementing nutrients to animals, one of them being that such supplements need to be palatable to the animal.
There is a requirement for an omega-3 rich supplement for animal use, especially for pet animals such as large breeds of dogs. For example, dogs like Labrador retriever, rottweiler, German shepherd and the like are more susceptible to osteoarthritis as demonstrated by its greater occurrence in these breeds. Additionally, dogs above the age of about 6 years have a significantly greater occurrence of osteoarthritis. Active dogs, athletic dogs and obese dogs can also be at risk. For the prevention of joint damage from osteoarthritis, regular supplementation rich in Omega-3 can help.
Although sufficient amount of Omega-3 can be consumed through diet, the most effective and easiest way is through dietary supplements. Administration of Omega-3 is a challenge, one issue is that consumers tend to move away from pills and prefer their supplements in form of food or beverages. With fish oil-based Omega-3 the challenge is greater, it is mostly available in the form of a soft gel capsule, it is hard to consume, and it has got bad taste and smell. One preferred alternative form is through eggs, nutrition enriched (NE) eggs and egg products can play a key role in this field.
Egg, whole or just yolk is utilized in the food industry for their functional and nutritional properties. For operational benefits, the eggs are used in dried form, the drying process leads to the removal of water and enhances the microbiological safety of the powders. The egg composition, particularly that of the yolk, depends on the age, breed, and diet of the bird. An egg is packed with vital nutrients like vitamins, proteins, minerals, lipoprotein, and essential fatty acids. Eggs are also rich in nutrients that most people know of yet do not consume enough of, such as Choline - which helps in building cell membrane; Lutein and Zeaxanthin - major benefits for eye health. Dried eggs retain most of the nutritional benefits of a fresh egg and they can be made part of dietary supplements for humans and animals.
Egg yolk nutritional composition can be altered by modifying a hen's diet. E.g., hens are fed flaxseed along with their feed to increase the Omega-3 content of the yolk as shown in US patent No. 5,897,890. In US patent No. 5133963 vacuum deodorized fish oil was added to poultry feed for producing commercially valuable quantities of poultry products. Again, in US patent No.5012761 chickens are fed composition including fish oil to produced modified egg rich in Omega-3. Fish waste is also blended with broiler feed to increase the Omega-3, especially EPA and DHA, content in the chicken meat as shown in US Patent App publication 20150237886. Although, Egg can act as a good carrier of Omega-3, but at the same time excess of a specific feed can affect the sensory properties of the eggs. Fish oil as poultry feed tends to leave a fishy odour and taste to the poultry product. Polyunsaturated fatty acids found in fish accumulate in the yolk, which spoils quickly once exposed to air, light, and heat. Spoilage produces volatile compounds a stronger fishy taste and smell.
OBJECTS OF THE INVENTION
An object of the invention is to provide an Omega-3 enriched composition comprising omega-3 enriched Egg powder which is stable for a long period of time and hence devoid of foul smell and is palatable to humans and animals and other functional nutrients derived plants.
One more objective of the invention is to derive the omega-3 enriched egg powder from Nutrient-Enhanced (NE) egg obtained from birds that are fed with fish oil and its derivatives along with their regular feed.
Another object of the invention is to provide a supplement formulation for improving cognitive skills, immunity, bone development, joint flexibility, eyesight, bone growth, regulate triglyceride levels, cardiovascular health, hormonal balance and fertility among humans and animals. The formulation can also prevent and treat infective diseases, inflammation among humans and animals.
Yet another object of the invention is a method to manufacture functional food comprising omega-3 enriched Egg powder and other functional nutrients derived plants.
SUMMARY
Application discloses an omega-3 enriched composition for using food supplements and medicinal applications. Said composition is comprising of omega-3 enriched Egg powder having eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) in the range of 0.4 to 8% (wt) of the egg powder. Composition further includes functional nutrients derived from plants selected from DAH (“diarylheptanoids” and “diphenylheptanoids” or “Curcumin-analogy) including, in any of their metal complexes or derivative forms (linear or cyclic) and citral in a ratio range of 99:1 to 1:99 (wt) and additives.
Disclosure further provides omega-3 enriched composition wherein omega-3 enriched egg powder is derived from Nutrient-Enhanced (NE) egg obtained from birds that are fed with fish oil and its derivatives along with their regular feed.
The disclosure provides omega-3 enriched composition containing 20% to 90% (wt) egg powder, 5% to 80% (wt) additives and 1% to 20%(wt) citral.
One aspect of the invention is an omega-3 enriched composition which contains 20% to 90% (wt) the egg powder, 5% to 80% (wt) additives, 1% to 20% (wt) DAH or its metal complexes thereof and 1% to 20% (wt) citral.
Another aspect of the invention is an omega-3 enriched composition which contains 20% to 90% (wt) the egg powder, 5% to 80% (wt) additives and 1% to 20% (wt) DAH or its metal complexes.
Disclosure further provides an omega 3 enriched water-soluble formulation containing whole egg 20-90% (wt), modified starch 5 to 30%, (wt) Pectin 1 to 10%(wt), and Aerosil 1 to 10% (wt).
Disclosure further provides an omega 3 enriched water-soluble formulation containing whole egg 20-90% (wt), 1% to 20% (wt) DAH or its metal complexes, modified starch 5 to 30%(wt), Pectin 1 to 10%(wt), and Aerosil 1 to 10% (wt).
Disclosure further provides an omega 3 enriched water-soluble formulation containing whole Egg solids 75±5% (wt), modified starch 14±5% (wt), Pectin 4±2% (wt), curcumin(ii)Ca complex 4±2% (wt) and Aerosil 3±1% (wt).
Disclosure provides an omega 3 enriched composition consisting of 5 to 80 % (wt) of additive selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination.
Disclosure provides an omega 3 enriched composition which has a moisture content of less than 10-5% and a peroxide value of 10-5 meq/Kg.
Disclosure provides an omega 3 enriched composition, said composition is free flowing, with a tapped density of 0.1-1 and has a particle size ranging from 100-0.1 to 1 um.
Disclosure provides an omega 3 enriched composition contain functional nutrients derived plants is a demethoxycurcumin complex of calcium, wherein said demethoxycurcumin complex of calcium is a curcuminoid (ii)Ca.
Another aspect of the disclosure is method of making the omega 3 composition, the method comprising the steps
a) collect the contents of the NE eggs either yolk or white or both in a vessel and stir well to a uniform mass, in a high shear mixer vessel;
b) take the required quantity of this solution corresponding to the specified total solids of the egg powder in the composition and mix it with the solution of modified starch (Hicap) with 28-30% TDS in a stirred vessel.
c) add required quantity of the Emulsifier to the resultant solution with constant stirring, until it is made uniform;
d) add required quantity of other functional nutrients derived from plants selected from diarylheptanoids, diphenylheptanoids or Curcumin-analogy (DAH) to the resultant solution with constant stirring so that a ratio in the range of 99:1 to 1:99 (wt) with the egg powder is maintained;
e) the resultant blended solution is passed through a high-pressure Homogeniser machine at 300 Bars pressure and
f) pasteurize the homogenized solution and Spray dry at 150oC.
Disclosure provides method of preparing an omega-3 enriched composition wherein contents of the nutrient enhanced eggs viz. yolk and white are separated and the yolk is pasteurized and then is spray dried to make into the powdered form, prior to adding the additives.
Disclosure provides method of preparing an omega-3 enriched composition wherein contents of the nutrient enhanced eggs viz. yolk and white are separated and the white is pasteurized and blended with additives 100:1 to 3:5 ratio and spray dried.
Disclosure provides method of preparing an omega-3 enriched composition wherein the contents of the nutrient enhanced eggs are collected in a vessel and stirred well to make a uniform mixture, the mixture is pasteurized and the pasteurized mixture is blended with an additive in 100:1 to 10:1 ratio and spray dried.
Disclosure provides method of preparing an omega-3 enriched composition wherein the nutrient enhanced egg is taken as a whole and the yolk and white is taken out and spray dried to get powdered egg powder.
Disclosure provides method of preparing an omega-3 enriched composition wherein the content of the egg is collected in a vessel and dissolved in an organic solvent to make a solution and the plant extract is added to form a mixture and resultant mixture is dried to obtain a nutrition enriched egg powder.
Disclosure provides method of preparing an omega-3 enriched composition wherein food grade additives that include but not limited to plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, fillers, and a combination thereof and the emulsifier includes but not limited to Lecithin, Beeswax, Candelilla wax, acylpolyols, acylpeptides, glycolipids, Xanthan Gum, Cetyl Alcohol, Stearyl Alcohol, Polyglycerols, agar, carrageenan, alginates, bean gum, gum Arabic, pectin, and guar gum and fillers include Maltodextrin, Pullulan, Pectin, Aerosil, white or brown sugar, coconut sugar, agave, honey, maple syrup, fruit juice concentrates, molasses, corn syrup, polysaccharides, and glycoproteins.
DETAILED DESCRIPTION THE INVENTION
The present invention is directed towards an Omega-3 composition and formulation comprising of avian egg rich in omega-3 having EPA and DHA. The composition further comprises diarylheptanoids, Curcumin-analogy, citral and other plant extracts. The composition is in a solid powdered form that could be made into a water-soluble formulation. The primary ingredient of Omega-3 composition is Nutrient-Enhanced (NE) egg powder. In some embodiment, said egg power is made from egg yolk only. In some embodiment, the egg powder is made of egg white only. In some embodiment, the egg yolk and egg white are separated and made into powder separately and blended in the desired ratio. The desired ratio might be for a specific formulation that requires specific nutrients in a specific ratio. Nutrient-Enhanced (NE) egg are obtained from birds that were fed fish oil and its derivatives along with their regular feed. The NE egg powder has about 10 to 20 times more Omega-3 than standard eggs.
There are three main omega-3 fatty acids, alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Omega-3 fatty acids are found in our foods, such as seafood, nuts, plant oils and fish oil. ALA is found mainly in plant oils such as flaxseed, soybean, chia seeds, walnuts, and canola oils. DHA and EPA are found mostly in fish, seaweed, and other seafood.
The term “DAH” means “diarylheptanoids” and “diphenylheptanoids” or “Curcumin-analogy”. This also includes any metal complex or derivatives forms of diarylheptanoids or diphenylheptanoids (linear or cyclic).
‘Composition’ and ‘Omega-3 composition’ are used interchangeably throughout the specification. If not specified ‘composition’ means ‘Omega-3 composition’.
The present invention is an egg-based Omega-3 composition and a process thereof. The Omega-3 composition is rich in omega-3, protein, fat, carbohydrate, calcium, and nutrients derived from plants and animals. The egg-based omega 3 composition of the present invention has a synergistic effect compared to normal egg. The composition is effective in improving liver function, recuperate hormonal balance, improving immunity, improving metabolism, prevent arthritis, improving skin radiance, enhance hair quality, reduce infection, prevent osteochondrosis and improving gut health.
In a preferred embodiment, the egg powder used in the composition is enriched with Omega-3, especially rich in EPA and DHA. The EPA and DHA range for 0.4 to 8% of the egg powder, more precisely a 100g egg powder has about 0.4g to 8g EPA and DHA. In some embodiment, the egg is also enriched with ALA.
In an embodiment, an Omega-3 composition is characterised with egg powder, and functional nutrients derived from plants and animals. The Omega-3 composition has a moisture content of less than 10-5% and a peroxide value of 10-5 meq/Kg. The composition is free flowing, with a tapped density of 0.1-1 and has a particle size ranging from 100 to 0.1 um. Characterized in the egg powder is Omega-3, especially Omega-3 rich in EPA and DHA. The EPA and DHA range for 0.4 to 8% of the egg powder, more precisely a 100g egg powder has about 0.4g to 8g EPA and DHA. In some embodiment, Omega-3 in the composition also includes ALA.
In an embodiment, an Omega-3 composition is characterised with egg powder, the egg powder is a blend of raw eggs with additives. The raw eggs could be whole egg or just the yolk or just the white. The EPA and DHA range for 0.4 to 8% of the egg powder, more precisely a 100g egg powder has about 0.4g to 8g EPA and DHA. In some embodiment, Omega-3 in the composition also includes ALA. The Omega-3 composition has a moisture content of less than 10-5% and a peroxide value of 10-5 meq/Kg. The composition is free flowing, with a tapped density of 0.1-1 and has a particle size ranging from 100-0.1 to 1 um. The additive used for the composition is selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination thereof, more specifically a combination of modified starches and an emulsifier.
In an embodiment, an Omega-3 composition is characterised with egg powder, the egg powder is a dehydrated raw egg. The raw eggs could be whole egg or just the yolk or just the white. The EPA and DHA range for 0.4 to 8% of the egg powder, more precisely a 100g egg powder has about 0.4g to 8g EPA and DHA. The Omega-3 composition has a moisture content of less than 10-5% and a peroxide value of 10-5 meq/Kg. The composition is free flowing, with a tapped density of 0.1-1 and has a particle size ranging from 100-0.1 to 1 um.
In an embodiment, an Omega-3 composition is characterised with egg powder, and functional nutrients such as DAH. In the composition DAH to Egg is in a 99:1 to 1:99 ratio by weight. The Omega-3 composition has a moisture content of less than 10-5% and a peroxide value of 10-5 meq/Kg. The composition is free flowing, with a tapped density of 0.1-1 and has a particle size ranging from 100-0.1 to 1 um. In a preferred embodiment, the DAH is a demethoxycurcumin complex of calcium (a preferred example is a curcuminoid (ii)Ca).
In yet another embodiment, an Omega-3 composition is characterised with egg powder, and functional nutrients derived from plants and animals. The composition contains egg powder about 20% to 90% by weight of the formulation and an additive about 5% to 80% by weight of the formulation. More preferably the egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder. The additive used for the composition is selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination thereof.
In yet another embodiment, an Omega-3 composition is characterised with egg powder, and functional nutrients such as DAH. The composition contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. More preferably the egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder. DAH to Omega-3 is in 99:1 to 1:99 ratio by weight. In a preferred embodiment, DAH is a metal complex of diarylheptanoids. The additive used for the composition is selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination thereof.
In yet another embodiment, an Omega-3 composition is characterised with egg powder, and functional nutrients such as citral. The composition contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a citral about 1% to 20% by weight of the formulation. More preferably the egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder. In a preferred embodiment, Citral is derived from lemongrass extract. The additive used for the composition is selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination thereof.
In yet another embodiment, an Omega-3 composition is characterised with egg powder, and functional nutrients such as DAH and Citral. The composition contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation DAH about 1% to 20% by weight of the formulation and Citral about 1% to 20% by weight of the formulation. More preferably the egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder. In a preferred embodiment, DAH is a metal complex of diarylheptanoids and Citral derived from lemongrass extract. The additive used for the composition is selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination thereof.
In yet another embodiment, a water-soluble formulation for an Omega-3 composition is disclosed, the formulation contains whole egg about 20-90%, modified starch about 5 to 30%, Pectin about 1 to 10%, and Aerosil about 1 to 10%. In some embodiment, DAH is added about 1 to 20% by weight of the formulation. The preferred DAH for this formulation is a soup of curcuminoid(ii)Ca complex. More preferably the formulation contains whole Egg solids about 75±5%, Hi-cap about 14±5%, Pectin about 4±2%, curcumin(ii)Ca complex about 4±2% and Aerosil about 3±1%.
Another aspect of the present invention is directed to a method to improving the health of humans and animals by administering a Nutrient-Enhanced (NE) egg product obtained from egg-producing birds. The NE egg products are rich in EPA and DHA and may also contain a high level of ALA, protein, calcium, phytochemicals, and other essential nutrients. The NE egg product could be in a powdered, granule, syrup, pellet, or tablet form. The NE egg could be a whole egg, powder egg or powdered egg combined with other nutrients.
In yet another embodiment, a method to help in brain development and cognitive skills of humans and animals, especially babies by administering a Nutrient-Enhanced (NE) egg product rich in EPA and DHA. NE egg product is more palatable than other sources of EPA and DHA, such as fish oil. More specifically administering a powdered formulation of omega-3 rich composition. The composition contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation, more preferably the egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In yet another embodiment, a method to improve gut health and gut microbiota in humans and animals by administrating an effective amount of Nutrient-Enhanced (NE) egg combined with DAH. NE eggs are rich in EPA and DHA. In some embodiment, citral is also combined with NE egg and DAH to make a composition suitable for the treatment and improvement of gut health and gut microbiota in humans and animals. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. In some embodiment citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In yet another embodiment, a method to improve liver function and cellular health in humans and animals is by administrating an effective amount of composition containing Nutrient-Enhanced (NE) egg combined with DAH. The composition further comprises citral. The NE egg products may contain a high level of EPA and/or DHA, protein and other essential nutrients. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. In some embodiment citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In yet another embodiment, a method to reduce the mortality rate of birds in poultry is by administrating a composition containing Nutrient-Enhanced (NE) egg and DAH. The composition can be fed along with poultry feed to improve the general health of the birds. Improvement in health includes but not limited to development and metabolism, growth and productive performance, immune response, and anti-oxidative properties, improving meat quality, bone growth and development, and improving fertility rates and semen quality. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and DAH about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In another embodiment, a method to prevent Necrotic Enteritis disease in poultry birds is by administrating an effective amount of composition containing Nutrient-Enhanced (NE) egg and DAH, said composition may further include citral. A specific amount of said composition can be included in the usual food ration daily, or the same amount can be provided to the animal in a treat or supplement daily. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. In some embodiment, citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In yet another embodiment, a method to prevent Coccidiosis disease in farm animals is by administrating an effective amount of composition containing Nutrient-Enhanced (NE) egg combined with DAH. The dietary composition further comprises citral. A specific amount of said composition can be included in the usual food ration daily, or the same amount can be provided to the animal in a treat or supplement daily. A combination of these or any other dosing means can be employed if an effective quantity of said composition is provided daily. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and DAH about 1% to 20% by weight of the formulation. In some embodiment citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In yet another embodiment, a method to prevent and reduce inflammation in humans and animals, more specifically in animals is by administering an effective amount of composition containing Nutrient-Enhanced (NE) egg combined with DAH. Said composition helps osteoarthritic dogs or dogs that might otherwise develop osteoarthritis, methods involving administration of the said composition to a dog for reducing the severity and frequency of clinical signs of osteoarthritis and the pain associated with this disease without substantial adverse reactions or side effects. This effect is in addition to any anti-inflammatory action of said composition, which may be of less importance in canine osteoarthritis because of limited involvement of inflammation in the osteoarthritis. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. In some embodiment citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
Osteochondrosis is a disturbance in endochondral ossification that is sometimes classified as dyschondroplasia. It may involve the separation of the immature articular cartilage from the underlying epiphyseal bone, which sometimes dissects completely free and floats loose in the synovial cavity and results in accompanying synovitis, or it may result in the retention of pyramidal cores of physeal cartilage projecting into the metaphysis. Often, these two lesions occur simultaneously in the same bone. The disease occurs during maximal growth when the biomechanical stresses are greatest in the immature skeleton (4-8 months in dogs). It is most common in large and giant breeds of dogs and rapidly growing pigs, horses, turkeys, and chickens.
In yet another embodiment, a method of slowing the clinical progression of an Osteochondrosis of an animal, the method is comprising administering an effective amount of composition containing Nutrient-Enhanced (NE) egg combined with DAH. In various embodiments, a method as described herein substantially improves the overall Osteochondrosis condition during the growth of the animal. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. In some embodiment citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In yet another embodiment, a method of slowing the clinical progression of an osteoarthritic condition of an animal, especially dogs, the method is comprising administering an effective amount of composition containing Nutrient-Enhanced (NE) egg combined with DAH. In various embodiments, a method as described herein substantially improves the overall osteoarthritic condition of the pet so that this benefit can be objectively measured through increased weight-bearing in osteoarthritic limbs. Dogs of primary interest include but not limited to German Shepherd, Labrador Retriever, Golden Retriever, Boxer, Briard, Beauceron, Weimaraner) and giant breeds of dogs (dogs over 45 kg, e.g. Great Danes, Saint Bernards, Rottweiler, Leonberger, Newfoundland, Great Pyrenees). More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. In some embodiment citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In yet another embodiment, a method to help in brain development of animal babies, boosting the immune system of animals, boosting the heart and kidney health of animals, improving the skin and coat health, reducing anxiety, depression, and hyperactivity by regularly administering an effective amount of composition containing Nutrient-Enhanced (NE) egg with DAH to animals. A specific amount of said composition can be included in the usual food ration daily, or the same amount can be provided to the animal in a treat or supplement daily. A combination of these or any other dosing means can be employed if an effective quantity of said composition is provided daily. More specifically, administering a powdered formulation of Nutrient-Enhanced (NE) egg. The formulation contains egg powder about 20% to 90% by weight of the formulation, additive about 5% to 80% by weight of the formulation and a DAH about 1% to 20% by weight of the formulation. In some embodiment citral is also added to the formulation about 1% to 20% by weight of the formulation. The egg powder is rich in EPA and DHA with EPA and DHA about 0.4 to 0.96% by weight of the egg powder.
In one embodiment, NE egg is taken, and egg yolk and egg white are separated, and the yolk is pasteurized and the is spray dried to make into powdered form. The white is pasteurized and blended with additives 100:1 to 3:5 ratio and spray dried.
In some embodiment the contents of the NE egg are collected in a vessel and stirred well to make a uniform mixture, the mixture is pasteurized. The pasteurized mixture is blended with an additive in 100:1 to 10:1 ratio and spray dried. In yet another embodiment, the NE egg is taken as a whole, and spray dried to get powdered egg powder. The additive used for the composition is selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination thereof. More specifically a combination of Modified starches and an emulsifier.
In yet another embodiment the content of the egg is collected in a vessel and dissolved in an organic solvent to make a solution. To the solution, plant extracts are added to form a mixture. The mixture is dried to obtain a nutrition enriched egg powder.
Examples of pets include dogs, cats, and rodents, e.g., chinchillas, guinea pigs, degus, mice, gerbils, hamsters, rats, ferrets, and lagomorphs, e.g., rabbits. Animals of all ages are included, e.g., young, adults, animals of medium age and seniors. Also includes farm animals like cow, buffalo, goat, pigs, horses, turkeys, chickens, duck, and quail.
Citral also known as lemonal is a pale-yellow liquid, with a strong lemon odour, that occurs in the essential oils of plants, such as lemongrass. It is insoluble in water but soluble in ethanol (ethyl alcohol), diethyl ether, and mineral oil. It is used in perfumes and flavourings and the manufacture of other chemicals. Chemically, citral is a mixture of two aldehydes that have the same molecular formula but different structures.
The composition is made with the help of additives. The term “additives” mean food grad additives that include but not limited to plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, fillers, and a combination thereof. Emulsifier includes but not limited to Lecithin, Beeswax, Candelilla wax, acylpolyols, acylpeptides, glycolipids, Xanthan Gum, Cetyl Alcohol, Stearyl Alcohol, Polyglycerols, agar, carrageenan, alginates, bean gum, gum Arabic, pectin, and guar gum. Fillers include Maltodextrin, Pullulan, Pectin, Aerosil, white or brown sugar, coconut sugar, agave, honey, maple syrup, fruit juice concentrates, molasses, corn syrup, polysaccharides, and glycoproteins.
The term “modified starch” means natural starch that has been physically or chemically modified, which includes but not limited to Acid-treated starch also called thin boiling starch, dextrin, alkaline-modified starch, bleached starch, oxidized starch, enzyme-treated starch, mono-starch phosphate, distarch phosphate, acetylated starch, hydroxypropylated starch, hydroxyethyl starch, starch sodium octenyl succinate (OSA) starch, starch aluminum octenyl Succinate, cationic starch, carboxymethylated starch, phosphate distarch phosphate, acetylated distarch phosphate, acetylated distarch adipate, hydroxypropyl distarch phosphate, and acetylated oxidized starch.
The biological materials used in the present application are avian eggs obtained from a poultry farm, in North Paravoor, Ernakulam District, Kerala State.
Example 1
Method of preparation of Water-soluble composition of omega 3 egg powder and DAH formulation.
66 Nutrient-Enhanced (NE) eggs were taken and whole contents of the eggs (yolk + white) were collected (3.4Kg) in a vessel and stirred well to a uniform mass, in a high shear mixer vessel. The total dissolved solids (TDS) were analyzed and TDS was found to be 23.5 and total solids were found to be 799g. 150g Hicap was added to the above solution and mixed well in a stirred vessel. To this solution, 43g pectin was added with constant stirring, until uniform. To this 53g, DAH was added with constant stirring. 32g aerosil was added to the above solution and the resultant blended solution was passed through a high-pressure Homogeniser machine at 300 Bars pressure. The homogenised solution was Pasteurised, and Spray dried at 150oC inlet temperature to form water soluble omega 3 enriched egg powder and DAH formulation with 75:5 (simplified ratio 15:1) ratio of omega 3 egg powder and DAH.
Example 2
Effect of omega 3 enriched egg powder formulation for the treatment of Osteoarthritis in Dogs.
5-6 years old Beagle dogs were selected from a group of dogs diagnosed as having Osteoarthritis for the study and are divided into 7 groups with 3 dogs in each group. Different omega 3 formulations/DAH (2.5g mixed with feed) and standard drug were fed to different groups for 2 months and dogs were checked at 15 days intervals. Dogs are classified as given below.
GP 1: Untreated control
GP 2: Dogs fed with standard drug PETJOINT and SHELCAL
GP 3: Dogs fed with omega 3 egg powder and DAH formulation
GP 4: Dogs fed with water-soluble omega 3 egg powder and DAH formulation
GP 5: Dogs fed with omega 3 enriched egg powder alone.
GP 6: Dogs fed with DAH alone.
GP7: Dogs fed with normal egg powder alone
At every 15 days interval, inflammation was checked by pain palpation at joints and X-ray of joints and blood serum calcium (Ca) levels were also checked.

Table 1: Effect of omega 3 enriched egg powder-DAH formulation for the treatment of Osteoarthritis
baseline 15th day 30th day 60th day
Pain X-ray Ca (mg/dL) Pain X-ray Ca
(mg/dL) Pain X-ray Ca
(mg/dL) Pain X-ray Ca
(mg/dL)
Gp1 D1 + + 5 + + 6 + + 5 + + 6
D2 + + 6 + + 5 + + 6 + + 6
D3 + + 6 + + 6 + + 6 + + 5
Gp 2 D1 + + 5 + + 6 + - 8 - - 9
D2 + + 4 + + 5 + - 9 - - 8
D3 + + 4 + + 4 + - 9 - - 8
Gp 3 D1 + + 6 + + 7 - - 8 - - 9
D2 + + 5 - + 6 - - 9 - - 9.5
D3 + + 6 + + 4 - - 9 - - 10
Gp 4 D1 + + 6 + - 6 - - 9 - - 9
D2 + + 6 + + 7 - - 10 - - 10
D3 + + 4 + + 5 - - 9 - - 10
Gp 5 D1 + + 6 + + 6 + + 6 + + 6
D2 + + 4 + + 6 + + 4 + + 6
D3 + + 5 + + 5 + + 6 + + 5
Gp6 D1 + + 5 + + 6 - + 7 - - 8
D2 + + 6 + + 7 - + 8 - - 9
D3 + + 6 + + 6 + + 7 - + 8
Gp 7 D1 + + 5 + + 5 + + 5 + + 6
D2 + + 4 + + 5 + + 5 + + 6
D3 + + 6 + + 6 + + 6 + + 5
*(+) -Pain and inflammation
*(-) No pain & inflammation
From the above table, it can be seen that groups 3 and 4 are not showing pain and inflammation on X-ray analysis of joints and palpation at joints after 2 months of treatment. On further examination of the said group, it is found that Calcium level in blood serum was also increased after 2 months of treatment. So, it is concluded that omega 3 enriched egg powder with DAH formulation is good for Osteoarthritis.
Example 3
Anti-inflammatory activity of omega 3 enriched egg powder formulation.
5-6 years old Beagle dogs were selected from a group of dogs diagnosed inflammation (hemiparaplegia) by testing C-reactive protein (CRP) level and CRP range more than 20mg/L were selected and divided into 7 groups with 3 dogs in each group. Different omega 3 formulations/DAH (2.5g mixed with feed) and standard drug prednisolone (30mg/day) were fed to dogs in different groups for 1 month. Dogs were checked for CRP level on the baseline (0th day) and 30th days. Dogs are classified as given below.
GP1- Untreated control
GP 2- Dogs fed with standard drug prednisolone
GP 3: Dogs fed with omega 3 egg powder and DAH formulation
GP 4: Dogs fed with water-soluble omega 3 egg powder and DAH formulation
GP 5: Dogs fed with omega 3 enriched egg powder alone.
GP 6: Dogs fed with DAH alone.
GP 7: Dogs fed with normal egg powder alone

Table 2: Anti-inflammatory activity of omega 3 enriched egg powder formulation.
GP1 GP 2 GP 3 GP4 GP5 GP6 GP7
CRP (mg/L) CRP (mg/L) CRP (mg/L) CRP (mg/L) CRP (mg/L) CRP (mg/L) CRP (mg/L)
Baseline D1 70 30 50 30 40 70 50
D2 40 50 70 60 30 60 60
D3 50 50 30 70 70 40 40
30th day D1 90 10 10 12 60 21 60
D2 100 9 12 10 40 18 80
D3 110 10 14 9 80 20 100

From the above table it can be seen that in Groups 3 and 4, CRP level was decreased below 20mg/L which indicates that omega 3 enriched egg powder and DAH formulation showed good anti-inflammatory effect. So, it is concluded that omega 3 enriched egg powder with DAH formulation showed good anti-inflammatory effect.
Example 4
Effect of Omega 3 enriched egg powder and DAH formulation on Immunity
Beagle dogs (1-2 years old) were selected from a group that are not given vaccination and divided into 7 groups with 3 dogs in each group. Different omega 3 formulations /DAH (2.5g mixed with feed) and standard drug Immunol were fed to dogs in different groups for 2 months. After 2 months of supplementation, parvovirus was induced in each group of dogs. Neutrophils, platelets and red blood cells (RBC) were checked on the baseline (0th day) and after 65th days of supplementation. Dogs are classified as given below.
GP1- Untreated control
GP 2- Dogs fed with standard drug Immunol
GP3: Dogs fed with omega 3 egg powder and DAH formulation
GP 4: Dogs fed with water-soluble omega 3 egg powder and DAH formulation
GP 5: Dogs fed with omega 3 enriched egg powder alone.
GP 6: Dogs fed with DAH alone.
GP 7: Dogs fed with normal egg powder alone
Table 3: Effect of Omega 3 enriched egg powder and DAH formulation on Immunity
Baseline (0 day) After 65th day
GP1 Neutrophils Normal High
Platelets Normal Low
RBC Normal Low
GP2 Neutrophils Normal Normal
Platelets Normal low
RBC Normal low
GP3 Neutrophils Normal Normal
Platelets Normal Normal
RBC Normal Normal
GP4 Neutrophils Normal Normal
Platelets Normal Normal
RBC Normal Normal
GP5 Neutrophils Normal High
Platelets Normal Low
RBC Normal Low
GP6 Neutrophils Normal High
Platelets Normal Normal
RBC Normal Normal
GP7 Neutrophils Normal High
Platelets Normal Low
RBC Normal Low
From the above table, it can be seen that GP3 and 4 shows strong immunity power after inducing parvovirus to dogs after 2 months supplementation.
We have brought out the novel features of the invention by explaining some of the preferred embodiments under the invention, enabling a person in the art to understand and visualize our invention. It is also to be understood that the invention is not limited in its application to the details set forth in the above description or illustrated in the drawings. Although the invention has been described in considerable detail with reference to certain preferred embodiments thereof, various modifications can be made without departing from the scope of the invention as described herein above and as defined in the appended claims. ,CLAIMS:1. An omega-3 enriched composition for using in food supplements and medicinal application comprising of omega-3 enriched Egg powder having eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) in the range for 0.4 to 8% of the egg powder and other functional nutrients derived from plants selected from DAH (“diarylheptanoids” and “diphenylheptanoids” or “Curcumin-analogy) including in any of their metal complexes or derivative forms (linear or cyclic) and citral in a ratio range of 99:1 to 1:99 by weight and additives.
2. The omega-3 enriched composition as claimed in claim 1, wherein omega-3 enriched egg powder is derived from Nutrient-Enhanced (NE) egg obtained from birds that are fed with fish oil and its derivatives along with their regular feed.
3. The omega-3 enriched composition as claimed in claim 1, wherein said composition contains 20% to 90% by weight the egg powder, 5% to 80% by weight additives and 1% to 20% by weight a citral.
4. The omega-3 enriched composition as claimed in claim 1, wherein said composition contains 20% to 90% by weight the egg powder, 5% to 80% by weight additives, 1% to 20% by weight DAH or its metal complexes thereof and 1% to 20% by weight a citral.
5. The omega-3 enriched composition as claimed in claim 4 and 5, wherein the citral is derived from lemongrass extract.
6. The omega-3 enriched composition as claimed in claim 1, wherein said composition contains 20% to 90% by weight the egg powder, 5% to 80% by weight additives and 1% to 20% by weight DAH or its metal complexes thereof.
7. The omega-3 enriched composition as claimed in claim 1, wherein a water-soluble formulation contains whole egg 20-90% (wt), modified starch 5 to 30%, (wt) Pectin 1 to 10%(wt), and Aerosil 1 to 10% (wt).
8. The omega-3 enriched composition as claimed in claim 1, wherein a water-soluble formulation contains whole egg 20-90% (wt), 1% to 20% by weight DAH or its metal complexes thereof, modified starch 5 to 30%, (wt) Pectin 1 to 10%(wt), and Aerosil 1 to 10% (wt).
9. The omega-3 enriched composition as claimed in claim 1, wherein a water-soluble formulation contains whole Egg solids 75±5% (wt), modified starch 14±5% (wt), Pectin 4±2% (wt), curcumin(ii)Ca complex 4±2% (wt) and Aerosil 3±1% (wt).
10. The omega-3 enriched composition as claimed in claim 1, wherein said composition consists of 5 to 80 % of additive selected from a group of plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, and a combination thereof.
11. The omega-3 enriched composition as claimed in claim 1, wherein said composition has a moisture content of less than 10-5% and a peroxide value of 10-5 meq/Kg.
12. The omega-3 enriched composition as claimed in claim 1, wherein said composition is free flowing, with a tapped density of 0.1-1 and has a particle size ranging from 100-0.1 to 1 um.
13. The omega-3 enriched composition as claimed in claim 1, wherein functional nutrients derived plants is a demethoxycurcumin complex of calcium.
14. The omega-3 enriched composition as claimed in claim 13, wherein said demethoxycurcumin complex of calcium is a curcuminoid (ii)Ca).
15. Method of preparing omega-3 enriched composition as claimed in claim 1 for using food supplements and medicinal application comprising the steps of :
a) collect the contents of the NE eggs either yolk or white or both in a vessel and stir well to a uniform mass, in a high shear mixer vessel;
b) analyze the total dissolved solids (TDS)
c) take the required Quantity of this solution corresponding to specified total sold of the egg powder in the composition and mix with modified starch (Hicap) with 28-30% TDS.
d) mix both these solutions in a stirred vessel;
e) add required quantity of the Emulsifier to the resultant solution with constant stirring, until it is made uniform;
f) add required quantity of other functional nutrients derived plants selected from diarylheptanoids, diphenylheptanoids or Curcumin-analogy (DAH) to the resultant solution with constant stirring so that a ratio in the range of 99:1 to 1:99 by weight with the egg powder is maintained;
g) the resultant blended solution is passed through a high-pressure Homogeniser machine at 300 Bars pressure and
h) pasteurize the homogenised solution and Spray dry at 150oC.
16. The method of preparing omega-3 enriched composition as claimed in claim 15, wherein contents of the NE eggs viz. yolk and white are separated and the yolk is pasteurized and then is spray dried to make into powdered form prior to adding the additives.
17. The method of preparing omega-3 enriched composition as claimed in claim 15, wherein contents of the NE eggs viz. yolk and white are separated and the white is pasteurized and blended with additives 100:1 to 3:5 ratio and spray dried.
18. The method of preparing omega-3 enriched composition as claimed in claim 15, wherein the contents of the NE egg are collected in a vessel and stirred well to make a uniform mixture, the mixture is pasteurized and the pasteurized mixture is blended with an additive in 100:1 to 10:1 ratio and spray dried.
19. The method of preparing omega-3 enriched composition as claimed in claim 15, wherein the NE egg is taken as a whole and the yolk and white is taken out and spray dried to get powdered egg powder.
20. The method of preparing omega-3 enriched composition as claimed in claim 15, wherein the content of the egg is collected in a vessel and dissolved in an organic solvent to make a solution and the plant extract are added to form a mixture and resultant mixture is dried to obtain a nutrition enriched egg powder.
21. The method of preparing omega-3 enriched composition as claimed in claim 15, wherein food grade additives that include but not limited to plant starch, modified starch, polysaccharides, glycoprotein, protein, sucrose, aerosol, emulsifier, fillers, and a combination thereof and the emulsifier includes but not limited to Lecithin, Beeswax, Candelilla wax, acylpolyols, acylpeptides, glycolipids, Xanthan Gum, Cetyl Alcohol, Stearyl Alcohol, Polyglycerols, agar, carrageenan, alginates, bean gum, gum Arabic, pectin, and guar gum and fillers include Maltodextrin, Pullulan, Pectin, Aerosil, white or brown sugar, coconut sugar, agave, honey, maple syrup, fruit juice concentrates, molasses, corn syrup, polysaccharides, and glycoproteins.