The present invention provides a process for isolating fermentable sugars from the acid hydrolyzate of a material containing cellulose, hemicellulose and lignin, particularly a process which is simple, economical and commercially viable.

Please refer the attachment

Please refer the attachment

The present invention provides for a nucleic acid encoding an isomerase and uses of the isomerase for bioconversion of sugar substrates. The invention represents an advancement in the field of enzyme engineering and discloses a modified nucleic acid for achieving optimum expression of a protein having isomerase acti...

The present disclosure provides methods for refolding isomaltulose synthase (ISase) of Pantoea dispersa UQ68J from inclusion bodies. The method comprises solubilizing inclusion bodies expressed in a host cell in a solubilization solution; diluting the solubilized sucrose isomerase to obtain a diluted sample; incubat...

The present invention provides a process for producing a low-calorie sugar composition in a bioreactor. The bioreactor comprises a pre-treatment reactor, a buffered tank, plurality of product storage tanks and columns having immobilized enzymes, wherein the enzyme columns are coupled in such a manner that each enzym...

The present invention provides for a novel whole-cell biocatalyst for the production of isomaltulose. The invention represents an advancement in the field of enzyme engineering and discloses a novel double deletion mutant whose invertase and permease encoding genes have been made inoperative. Further, the mutant hos...

The present invention provides for a novel whole cell biocatalyst for the production of trehalulose. The invention represents an advancement in the field of enzyme engineering and discloses a novel double deletion mutant whose invertase and permease encoding genes have been made inoperative. Further, the mutant host...

METHODS FOR REFOLDING SUCROSE ISOMERASE ABSTRACT The present disclosure provides methods for refolding sucrose isomerase (SIase) of Pseudomonas mesoacidophila MX-45 from inclusion bodies. The method comprises solubilizing inclusion bodies expressed in a host cell in a solubilization solution; diluting the solubili...

The present invention is directed towards genetic modification of native gene encoding for D-tagatose 3-epimerase and rhamnose isomerase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare mono-saccharides such as psicose and allose. Also disclosed in ...

The present invention is directed towards genetic modification of native gene encoding for sucrose isomerase and isomaltulose synthase to substantially increase the expression level of these enzymes and use of said enzymes in a process to produce rare disaccharides such as isomaltulose and trehalulose. Also disclose...

The present invention provides a D-psicose-3-epimerase mutant for efficient and effective production of D-allulose (also known as D-psicose) from D- fructose. The invention represents an advancement in the field of enzyme engineering. The mutant enzyme has increased thermo-stability, operational stability and is fun...

The present disclosure provides a system and a method for increasing concentration of sugar in an aqueous solution. The system and method are described in detailed in the detailed description.

The present disclosure relates to a system and a method for crystallizing allulose from an allulose solution.

The present disclosure relates to a system and method for purifying D-allulose and/or fructose from a binary mixture.

The present disclosure relates to a system and method for separating and/or purifying D-allulose from a ternary mixture.