Promoter Variants

Abstract: An isolated and/or artificial pG1 x promoter which is a functional variant of the carbon source regulatable pG1 promoter of Pichia pastoris identified by SEQ ID 1 which pG1 x promoter consists of or comprises at least a part of SEQ ID 1 with a length of at least 293 bp characterized by the following promoter regions: a) at least one core regulatory region comprising the nucleotide sequences SEQ ID 2 and SEQ ID 3; and b) a non core regulatory region which is any region within the pG1 x promoter sequence other than the core regulatory region; wherein the pG1 x promoter comprises at least one mutation in any of the promoter regions and a sequence identity of at least 80% in SEQ ID 2 and SEQ ID 3 and a sequence identity of at least 50% in any region other than SEQ ID 2 or SEQ ID 3; and further wherein the pG1 x promoter is characterized by the same or an increased promoter strength and induction ratio as compared to the pG1 promoter wherein  the promoter strength is at least 1.1 fold increased in the induced state as compared to the pG1 promoter and/or  the induction ratio is at least 1.1 fold increased as compared to the pG1 promoter.

Patent Information

Applicants

Inventors

Specification

Documents

Application Documents

Search Strategy