The present invention relates to a chimeric protein of recombinant human granulocyte colony stimulating factor comprising of hG-CSF fused to an affinity fusion tag. The affinity tag that is employed in the present invention is Maltose binding protein.

The present invention provides method for producing granulocyte colony stimulating factor by constitutive cytoplasmic expression in Pichia pastoris using the promoter from the glyceraldehyde-3-phosphate dehydrogenase gene.

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The present invention provides the use of acid pretreated biomass such as jatropha seed cake, pongamia seed cake and banana stems as renewable feedstock for butanol production by the mutant Clostridium acetobutylicum MTCC 587. Chemical mutagenesis was carried out for improvisation of the strain for better butanol to...

Disclosed herein are methods and compositions related to the production and extraction of oils and biodiesel from oleaginous yeast, such as Cryptococcus curvatus. Also disclosed herein are methods for providing fermentation conditions for the production of yeast in high density using inexpensive raw materials includ...

The present invention provides the morphogenic potential of immature embryo cultures Jatropha sp.. The present invention in particular provides a reproducible, genotype independent protocol for high frequency shoot regeneration from immature embryos of Jatropha sp. 28 1 3 FEB 2009

The present disclosure provides methods useful for screening compounds and/or compositions, for example potential drug candidates. The results of the screening assays correlate to the effects of the compounds on the molecular and/or cellular level of the human body. Also disclosed are screening assays utilizing huma...

A disposable device is provided for culturing and/or packaging and transporting ready-to-use viable cells cultured on membranes, gels or micro porous substrata. The device includes a housing base defining an interior for culturing cells. The membrane to be used for culturing the cells is placed inside the housing ba...

The present invention provides pluripotent corneal stem cells from limbal biopsies, cultured ex vivo, and purified by magnetic affinity cell sorting (MACS). These cells possess multilineage differentitation potential and self-renewing capability. They could be propagated in culture, where they continuously produce c...

The present invention provides pluripotent corneal stem cells from limbal biopsies, cultured ex vivo, and isolated and purified by magnetic affinity cell sorting (MACS). These cells possess multilineage differentiation potential and self-renewing capability. They could be propagated in culture, where they continuous...

The present invention relates to the recombinant fusion protein of interferon. More particularly the present invention relates to the recombinant fusion proteins of interferon with affinity fusion tag.

The present invention relates to the differentiation of cardiomyocytes from a human embryonic stem cell line, for example the ReliCell®hES1 cell line. The present invention in particular relates to the delivery of these cells via non-invasive routes like intravenous routes and its potential applications in cardiac d...

The disclosure provides methods and systems for the production of biodiesel from biological feedstocks such as vegetable oils or animal fats. In particular, the disclosure is directed to the product of biodiesel from seeds such as non-edible oil seeds. Methods for maximizing oil recovery from the feedstocks are pro...

The present invention relates to oral Controlled release preparation of insulin encapsulated hydrogen micro particles and/or micro particles produced by micro encapsulation of insulin using the newly developed copolymers, polymers, blend polymers and its method.

The present invention involves a device for culturing cells on removable membranes/sponges for the purpose of transplantation. The device includes a base plate with a snaps and a ring, which fits into the base plate. The membrane/sponge of choice for culturing cells is placed on the base plate and fixed by the ring,...

The present invention relates to the efficient propagation of Punica granatum wherein the in vitro protocol for mass multiplication comprises nutrient salts, growth regulators and optimal culture conditions. The multiplication rate observed was higher with the protocol derived.

The present invention relates to an efficient process for producing Poly Lactic Acid from fermentation of renewable agricultural feed-stocks not limited to molasses or cane bagasse employed as starting material. The present invention in particular provides a cost effective, and industrially scalable process for prod...

The present invention relates to a process of disinfecting biological materials. In particular, the invention relates to a novel process for removing detergent and/or solvent added to biological material. This present invention provides a safe, efficient, and economical method of removing virucidal agents viz. solve...

This invention provides an efficient process for in vitro propagation of Jatropha through direct regeneration of leaf disc without any intermediary callus phase. This process provides a commercially viable process for producing true-to-type plants of Jatropha on a large scale.

ABSTRACT Purified preparations of human embryonic stem cells with certain population-specific characteristics are disclosed. This preparation is characterized by the positive expression of the following pluripotent cell surface markers: SSEA-1 (-); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); alkaline phosphatase (+), a...

A method of culturing mesenchymal stem cells derived from umbilical cord tissue (UCMSC) comprising a. collecting umbilical cord tissue; b. plating a piece of umbilical cord tissue into a tissue culture flask comprising a culture medium along with cord blood serum for about 3-4 days to produce adhered cell cultu...

The present invention relates to methods of increasing oil content in Jatropha Curcas L. plants. The invention further provides a partial and complete cDNA sequence encoding Jatropha acetyl CoA carboxylase (ACCase) and methods for cloning and expressing the Jatropha ACCase gene to produce transgenic plants with incr...

Novel benzoquinone-derived compounds and polymorphs and prodrugs thereof, geometric or optical isomers thereof, and pharmaceutically acceptable esters, ethers, carbamates, oximes of such compunds, polymorphs, prodrugs and isomers are provided. process for preparation of compounds of the invention and pharmaceutical ...

The  present  invention  relates  to  an  efficient  process  for  preparing  an arylcyclopropanecarbonitnle, which involves the use of sulfolane as a solvent.

The present invention provides an efficient and improved process for the production and purification of high molecular weight Hyaluronic acid(HA). One embodiment relates to providing growth condituions for production of high molecular weight HA in streptococcus zooepidemicus. For example, growth in medium comprising...

The invention provides carbohydrate based molecules, methods of preparation, and composition, useful of modulating signaling through Toll-like receptors. The methods involve contacting a TLR-expressing cell with a carbohydrate based molecules having a core structure comprising of one or more sugar molecules. These c...

The present invention relates to novel synthetic toll like receptor antagonist. The present invention in particular provides compounds, methods and compositions for specifically inhibiting immune stimulation involving TLR ligands, especially TLR-4. The compounds are potentially useful in treatment of inflammation, a...

Disclosed herein are methods and compositions related to the production and extraction of oils and biodiesel from oleaginous yeast, such as a new yeast isolate of the genus Pichia. Also disclosed herein are methods for providing fermentation conditions for the production of yeast in high density using inexpensive ra...

The present invention relates to the tissue system with self-regenerating pluripotent limbal stem cells, comprising of large population of undifferentiated limbal stem cells amounting to more than 70% suitable for restoring ocular surface impairments particularly with severe limbal stem cells deficiency. The improve...

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The present invention relates to the commercially viable for in-vitro mass culture of Chlorophytum borivilianum. The present invention process for in-vitro mass culture of Chlorophytum borivilianum is simple, faster and suitable for production of disease free root tubers of uniform quality. The process of the presen...

The present describes a tissue system with conjunctival cells, including conjunctival stem cells. The conjunctival tissue system is derived from isolated tissue comprising conjunctival cells, and is suitable for restoring ocular surface impairments, particularly those that result from damaged or diseased conjunctiva...

The present invention relates to an interactive wound cover wherein a cultured monolayer of keratinocytes is delivered using a biopolymer. The present invention describes the composition, method of preparation and its properties relating to safety, and efficacy. The wound cover of the present invention is useful in ...

The present invention relates to a commercially viable process for in vitro mass culture of Jatropha curcas. The present invention process for in vitro mass culture of Jatropha curcus is simple, faster, and suitable for production of disease-free root tubers of uniform quality. The process of the present invention f...

The present invention relates to aerobic production of 1,3-Propanediol by Klebsiella pneumoniae ATCC 15380. The process in particular provides an efficient conversion of glycerol to 1,3-Propanediol which is stoichiometrically over 0.9 mol of propanediol/mol of glycerol compared to 0.85 mole/mole theoretical yield un...

The present disclosure relates to pH-sensitive copolymers poly(methacrylic acid-co-N-vinylcaprolactam), designated as P(MAA-co-VCL). The disclosure in particular provides the synthesis of a copolymer of poly(methacrylic acid-co-N-vinylcaprolactam) and its application for pH sensitive oral delivery of active ingred...

The present invention provides methods for 1, 2-asymmetric reduction of other compounds wherein 1,2- trans diol is present. In particular the present invention discloses preparation of 17(3-Estriol and its derivatives having substantially free of 17a-Estriol impurity using an achiral reductant sodium triacetoxy boro...

PROCESS FOR THE PREPARATION OF ESTRADIOL AND ITS DERIVATIVES The present invention provides a process for the preparation of a compound of formula I wherein R|1represents a hydrogen atom, halogen atom, cyano group, hydroxyl group, straight or branched C1-C10 alkyl group, straight or branched C2-C10 alkenyl grou...

This invention relates to the use of short interfering nucleic acid molecules (SIRNA) in modulation of Nuclear Mitotic Apparatus Protein (NuMA) gene expression. The invention in particular related to the compounds, compositions and methods useful for modulation of expression and activity of other genes involved in p...

The present invention is a method for the preparation of a kit for amplifying Hepatitis C Virus (HCV)in which the amplimers are transcripts of a polyprotein gene of HCV. The amplicons are hybridized to a specific oligonucleotide probe, which allows the amplicons to be detected.

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The invention provides an efficient dual mode fermentation process comprising of employing free cell population as well as cells immobilized on an immobilizing means for fermenting the suitable carbohydrate source to produce various metabolites particularly lactic acid with improved efficiency of conversion rate of ...

The present disclosure describes methods for identifying chromosomal abnormalities in cells obtained from follicular fluid to identify,for examlpe,gonadal mosaicism or low-grade gonadal mosaicism.The chromosomal abnormalities are identified using genetic analysis that can chromosomal,DNA or gene expression abnormali...

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The present invention provides an improved process for the preparation of alpha form of imatinib mesylate with (long needle) and a- crystal form (small needle) in a consistent manner and novel alpha crystal forms of imatinib mesylate. The present invention in particular provides a reproducible and efficient process....

The present invention relates to an efficient and improved process for purifying a recombinant protein. The invention relates to the purification of tissue plasminogen activator (tPA), such as truncated human tPA, recombinantly produced in bacteria, for example in E. coll. The present invention provides a proc...

The present invention provides a method for culturing mesenchymal stem cells using cord blood serum, for therapeutic purposes in regenerative medicine; and in particular the present invention provides the use of these cells in the treatment of Parkinson"s disease, and the present invention has provided proliferation...

The present invention provides a novel method for identifying genes in Jatropha plants, such as Jatropha curcas L., that confer tolerance to abiotic stress such as salinity, drought, and ion stresses. Such genes allow the development of Jatropha curcas cultivars that display enhanced salinity and stress tolerance, w...

The present invention relates to an improved process of production of high yield of butanol using Clostridium acetobutylicum ATCC 10132. The present invention in particular reports a strain with enhanced butanol tolerance under the optimized conditions.

In vitro culture of mesenchymal stem cells (MSC) and a process for the preparation thereof for therapeutic use. The present invenion provides a method for culturing mesenchymal stem cells using cord bolld serum, for therapeutic purposes in regenerative medicine. In particular the present invention provides the use...

The present invention provides a process for the preparation of a tetrazine derivative of formula (I), or a pharmaceutically acceptable salt thereof wherein R2 Ri represents a hydrogen atom, a straight or branched Ci-Ce alkyl group, C2-C6 alkenyl group or C2-C6 alkynyl group, which Ci-Cg alkyl group, C2-C6 alke...

The invention relates to the use of short nucleic acid molecules (siNAs, such as siRNAs) that modulate the expression of VEGF-C and/or RhoA involved in neovascular angiogenesis. In the present invention, inhibition of VEGF-C and/or RhoA gene expression lead to decreased expression of VEGF-A, which is required for in...

The present invention relates to the hydrogel compositions and it methods of preparation. The invention in particular provides hydrogel composition of PVA and PEG having enhanced water absorption capacity and mechanical strength.

The present invention relates to Poly(N-vinyl caprolactum co-acrylamide) copolymers prepared by free radical emulsion polymerization from acrylamide (AAm) and TV-vinyl caprolactum (VCL) monomers with N,N-methylene bisacrylamide (NNMBA) as the crosslinking agent. In particular, the present invention provides the appl...

The present invention relates to improved process of producing high yield of Polymyxin B sulphate by fermentation. The present invention m particular provides an optimized fermentation conditions for production of Polymyxin B.. - 11 - 24 NOV2008

The present invention deals with a protein and compositions comprising the same for inhibition of lipases and phospholipases in the body fluids, cells and tissues for the prevention and treatment of metabolic syndrome, cardiovascular disorders, and inflammatory diseases. The protein is either isolated from plant spe...

The present invention relates to the method for synthesis of human recombinant insulin in E.coli through a recombinant plasmid construct having affinity tag. The affinity tag employed in the present invention is T7 tag having 11 amino acid residues. In the present invention with the use of T7 tag CNBr would not be r...

The present invention provides improved process for preparation of recombinant human granulocyte colony stimulating factor (hG-CSF) by constitutive expression in Pichia pastoris. The present invention more particularly provides improved process for preparation of recombinant human granulocyte colony stimulating fact...

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The present disclosure describes the generation of neural cells and neurons from mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. Specifically, the present disclosure describes the generation of neuroprogenitor cells and differentiated dopaminergic ...

The present disclosure describes the generation of neural cells and neurons from mammalian pluripotent embryonic-like stem cells (ELSCs) isolated from corneal limbal tissue, a non-embryonic tissue. Specifically, the present disclosure describes the generation of neuroprogenitor cells and differentiated dopaminergic ...

The present invention provides an improved process for the production of gibberellic acid (GA3), achieving a yield over 225 g/kg of GA3 with solid substrate fermentation, and over 15g/L submerged fermentation. The method also provides novel substrates, including the use of Jatropha seed cake. The present invention h...

The invention in particular relates to the production and expression of Interleukin 1 receptor antagonist (IL-lra) in Pichia Pastoris. The present invention in particular relates to a engineered Pichia strain x-33/pGAPZ/IL-lRa constructed by cloning the gene in pGAPZA and transforming the recombinant vector into Pic...

The present invention relates to a graft wherein cultured autologous melanocytes are delivered using a biopolymer. The present invention describes the composition, method of preparation and its properties relating to safety, and efficacy. The graft of the present invention has a potential use in repigmenting skin ...

Novel compounds, their methods of preparation and use in therapies related to cancer and inflammation are provided. Compounds comprise esters of cinnamic acid, vanillic acid and 4-hyrdoxy cinnamic acid and derivates and salts thereof. Compounds with novel benzofuran lignan structure as a potent antimitotic agent and...

The present invention provides a three-dimensional tissue equivalent for in-vivo and in-vitro uses. The there dimensional tissue equivalent of the present invention is a non-contractile cellular sheet cultured over a porous scaffold by a specially designed process wherein the cell sheet is entirely on one side of th...

This invention relates to the use of short nucleic acid molecules that modulate RhoA -kinase (RhoA) expression. The invention includes compounds, compositions and methods useful for silencing the expression and activity of the RhoA gene involved in the RhoA kinase pathway. In one embodiment, the present invention pr...

The present invention provides a three-dimensional tissue equivalent for in-vivo and in-vitro uses. The three dimensional tissue equivalent of the present invention is non-contractile cellular sheet cultured over a porous scaffold using a macromass culturing technique, for example where the cellular sheet is entirel...

The present invention relates to efficient constitutive expression of rHGH in Pichia pastoris using a GAP promoter, yielding around 10-300 mg/L extracellularly in 24-72 hours of fermentation.

The present invention provides expressed sequence tags (ESTs) and contigs (comprising the sequence of two or more ESTs) of Jatropha curcas and its applications in isolating full length genes, identifying genes involved in different biotic and abiotic stresses, and determining gene function.

The present invention is related to a device which is a three dimensional bio-reactor for expansion of haemopoietic stem cells derived from human Umbilical Cord Blood for therapeutic use,without compromising are self-renewal property of the cells.The process of self -renewal and differentiation are regulated...

. A method for establishing cell lines from the inner cell mass of a human blastocyst, comprising the steps of:(a) isolating a blastocyst; comprises zona pellucida, trophectoderm and inner cell mass.(b) creating an aperture in the blastocyst by laser ablation; and(c) Isolating cells of th...

An efficient method of in-vitro micropreparation of piper longum from lateral bud explant, said method comprising the steps of:-i. cutting the lateral buds from the stem segments under laminar flow hood and dipping them in hydrogen chloride (0.002 - 0.2%) for 3 minutes and rinsing it with autoclaved di...

A method for detecting amplimers of Human Cytomegalovirus Veremia (hCMV)genome with oligonucleotide probe comprising of:i) designing oligonucleotide probe capable of capturing amplimers of hCMV genome and labeled at 5' end;ii) adding biotinylated oligonucleotide probe about lμl to about 10 ...

A method for growing neural precursor cells from the umbilical cord blood fortherapeutic purposes, comprising the steps of:i. Isolating the stem cell and progenitor cell population from mononuclear cellsseparated from umbilical cord blood, using a magnetic cell separator toseparate ou...

A highly efficient in-vitro system of micropropagation of rose scented Geranium, Pelargonium graveolens L. Herit Bourbon type by direct regeneration method toproduce large number of viable true to the type plants maintaining the genotype of■■■> an elite mother plant is provided. The process invol...

ABSTRACT OF THE INVENTION:Solarium viarum is an alkaloid producing plants of the family Solanaceae with varied therapeutic uses. In order to grow the plant in large areas; one needs to have an efficient system of vegetative multiplication, which ensures its genetic uniformity, and true to the type natu...

The present invention relates to the improved mehtod for production of insulin in Pichia pastoris using the promoter from the glyceraldhyde-3-phosphate dehydrogenase gene for constitutive expression. The use of glyceraldehyde-3-phosphate dehydrogenase gene in the present invention facilitates tjhe consittuti...

The present invention provides fusion proteins of Calcitonin with Interleukin 2 that have enhanced biological activity compared to Calcitonin. In particular, the present invention provides the process of preparing and using compositions of the fusion protein of Calcitonin with Interleukin 2. Also provided ar...

This invention relates to the use of short nucleic acid molecules that modulate Aurora-B kinase (AurkB) expression. The invention includes compounds, compositions and methods useful for the modulation of expression and activity of genes involved in the AurkB pathway. In one embodiment, the present invention ...

The present invention provides a stable immunogenic composition and a process for preparation thereof. The immunogenic composition comprises recombinant proteins suitable for SARS-CoV-2. More specifically, the present invention provides a composition comprising a receptor binding domain protein and a nucleocapsid pr...